Posts Tagged ‘genetic background’

Genetic differences among C57BL/6 mouse strains available from the Mouse Phenome Database

Monday, April 9th, 2012
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Genetic differences among C57BL/6 mouse strains
Genetic differences among C57BL/6 mouse strains

The Mouse Phenome Database (MPD), at the Jackson laboratory web site, has recently updated their list of SNP polymorphisms for closely related strains across the entire mouse genome. The premade lists, genome-wide, ready-to-use, now include three NEW comparisons containing all currently known genetic differences (SNPs) among C57BL/6 strains, particularly between C57BL/6J and C57BL/6N mouse strains, and their related substrains (entire genome occurrences).

  1. (coding and UTR regions only, 4341 SNPs)

  2. C57BL/6J versus C57BL/6NJ (all available SNPs that are polymorphic, 152001 SNPs)
  3. 13 C57BL/6 mouse related strains, J and N, including: C57BL/6J, C57BL/6ByJ, C57BL/6JArc, C57BL/6JBomTac, C57BL/6JCrl, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRccHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NNIH, C57BL/6NTac

The entire community of researchers in biomedicine using mouse genetics greatly appreciates the most useful service and helpful web resources provided by the MPD team. Thanks a lot colleagues!

Genome sequencing of 17 mouse inbred strains

Friday, September 16th, 2011
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Mouse genomic variation and its effect on phenotypes and gene regulation. Keane et al. Nature 2011, 289–294,  (15 September 2011), doi:10.1038/nature10413

Mouse genomic variation and its effect on phenotypes and gene regulation. Keane et al. Nature 2011, 289–294, (15 September 2011), doi:10.1038/nature10413

Most of our current understanding and genome research in mice has been based, so far, on the genome of the mouse inbred strain C57BL/6J, published in 2002 and accessible through a number of genome browsers, such as Ensembl. Yesterday, Keane et al. published in Nature the results of their sequencing efforts and the release of the genome sequences from 17 additional mouse inbred strains: C3H/HeJ, CBA/J, A/J, AKR/J, DBA/2J, LP/J, BALB/cJ, NZO/HlLtJ, NOD/ShiLtJ, 129S5SvEv<Brd>, 129P2/OlaHsd, 129S1/SvImJ, C57BL/6NJ, and the four wild-derived inbred strains CAST/EiJ, PWK/PhJ, WSB/EiJ and SPRET/EiJ. This is an enormous amount of information, obtained using next-generation sequencing, that these researchers from The Wellcome Trust Sanger Institute and other collaborating institutions now release to the public scientific community. Most of these inbred strains are very relevant in biomedical research and they are regularly used for the generation of genetically-modified mice. Notably, among them the C57BL/6NJ inbred strain, selected by the International KnockOut Mouse Consortium (IKMC) to produce the collection of systematic gene knockouts covering the entire mouse genome. This publication and the subsequent analyses derived from this study should greatly contribute to our understanding of the genetic diversity among different mouse strains and the different phenotypes that are often observed across inbred strains, associated with identical genetic modifications.

Course and Symposium on Cryopreservation at CEMIB, Campinas, Brazil

Friday, November 19th, 2010
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Course and Symposium on Cryopreservation at CEMIB, Campinas, Brazil

Course and Symposium on Cryopreservation at CEMIB, Campinas, Brazil

The Course on Cryopreservation of Mouse Germoplasm and the associated Symposium on Animal Models and Cryopreservation will be held next week, starting Monday November 22, until Friday, November 26, at the Multidisciplinary Center for Biological Investigation on Laboratory Animal Science (CEMIB), University of Campinas, Campinas, Brazil. This course and symposium are co-sponsored by the International Society for Transgenic Technologies (ISTT).

The Organizing Committe of these initiatives include several ISTT Members: Delma Pegolo Alves (CEMIB, Director), Marcus Corat (CEMIB), Jorge Sztein (NEI-NIH, Bethesda, MD, USA) and Martina Crispo (Institut Pasteur Montevideo, Uruguay). In addition to these persons, the scientific program  also include additional ISTT Members as invited speakers : Fernando Benavides (MD Anderson Cancer Center, Smithville, TX, USA) and Lluís Montoliu (CNB-CSIC, Madrid, Spain). The list of faculty members teaching this course is completed with the contributions from: Humberto Rangel (CEMIB founder), Jean Louis Guénet (Inst. Pasteur, Paris, France), Luiz Augusto Corrêa Passos (CEMIB) and Flaminio de Oliveira Rangel (CEMIB).

Genetic polymorphisms among C57BL/6 mouse inbred strains

Monday, May 31st, 2010
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Genetic polymorphisms among C57BL/6 mouse inbred strains

Genetic polymorphisms among C57BL/6 mouse inbred strains

A manuscript entitled “Genetic polymorphisms among C57BL/6 mouse inbred strains” has been just published by Esther Zurita, Mónica Chagoyen, Marta Cantero, Rosario Alonso, Anna González-Neira, Alejandro López-Jiménez, José Antonio López-Moreno, Carlisle P. Landel, Javier Benítez, Florencio Pazos and Lluís Montoliu in Transgenic Research. The Illumina® Mouse Medium Density Linkage Mapping panel, with 1,449 single nucleotide polymorphisms (SNPs), was used to genotype individuals from ten C57BL/6-related strains: C57BL/6JArc, C57BL/6J from The Jackson Lab, C57BL/6J from Crl, C57BL6/JRccHsd, C57BL/6JOlaHsd, C57BL/6JBomTac, B6(Cg)-Tyr (<c-2j>)/J, C57BL/6NCrl, C57BL/6NHsd and C57BL/6NTac. Twelve SNPs were found informative to discriminate between the “/N” and “/J”  C57BL/6 mouse substrains considered. These results will be instrumental for the correct genetic monitoring and appropriate mouse colony handling of different transgenic and knockout mice produced in distinct C57BL/6 inbred substrains. The SNP raw data are also available from the Mouse Phenome Database at Jackson Laboratory.


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