Posts Tagged ‘EMMA’

Laboratory Course on Cryopreservation of Mouse Germplasm, Monterotondo, Italy, 20-24 October 2014

Friday, April 11th, 2014
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Laboratory Course on Cryopreservation of Mouse Germplasm, Monterotondo, Italy, 20-24 October 2014

Laboratory Course on Cryopreservation of Mouse Germplasm, Monterotondo, Italy, 20-24 October 2014

The Consiglio Nazionale delle Ricerche (CNR), EMMA-Monterotondo Campus International Development and The Jackson Laboratory (JAX) offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. This Course is financially supported by the European Union FP7 Capacities Specific Program, through the Infrafrontier-I3. The 2014 Edition of this traditional annual cryopreservation EMMA-JAX course is planned for 20-24 October 2014, organized by ISTT Member Marcello Raspa and his collaborators at CNR-EMMA Campus and with the participation of various faculty members, including Robert Taft and Jane Farley, from JAX; Ferdinando Scavizzi and Raffaele Matteoni, from CNR; Susan Marschall, from Munich; Martin Fray, from Harwell; Lluis Montoliu, from Madrid; and Kent Lloyd, from UC Davis, USA.

The 2014 edition of this course will be organized in memory of Stanley P. Leibo – Course leader 1999-2013.

The course is offered to teach methods in cryopreservation for banking of various strains of mice used in research, including inbred, transgenic and knock-out strains. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a “hands-on” laboratory program in which participants learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries. Techniques include: embryo “two-step” equilibrium freezing in plastic straws, embryo non-equilibrium “ultra-rapid” cooling or “vitrification” in straws and sperm freezing and recovery of frozen sperm by in vitro fertilization. In addition, general principles of cryobiology, development of inventory databases for individual programs, and adaptation of long-term storage systems and cryogenic equipment for different situations will be presented and discussed.

Enrollment is limited to 10 participants and early application is advised. This Course is financially supported by the EU FP7 Capacities Specific Program, through the Infrafrontier-I3.

Course Fees (Fees do not include participant’s travel and lodging):

European Union’s and Associated Countries’ nationals:

  • Academic or Non-Profit Institutions: Euro 400.00
  • Other Institutions: Euro 950.00

Other Countries’ nationals:

  • Academic or Non-Profit Institutions: Euro 900.00
  • Other Institutions: Euro 1,200.00

Deadline for applications: September 10, 2014
Download here the Course application form

Course information:
Marcello Raspa, Ferdinando Scavizzi, Raffaele Matteoni
Consiglio Nazionale delle Ricerche
EMMA-Monterotondo Campus International Development
INFRAFRONTIER I3
e-mail: mraspa@emma.cnr.it

Course secretariat:
Giuliana Boera, Teresa Cuccurullo
Consiglio Nazionale delle Ricerche
EMMA-Monterotondo Campus International Development
INFRAFRONTIER I3
e-mail: cuccurullo@ibc.cnr.it

In memoriam: Stanley Leibo

Tuesday, March 25th, 2014
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Stanley Leibo in Rome, October 2013, at the official dinner of the annual EMMA-JAX cryopreservation course in Monterotondo, Italy (Photograph kindly provided by Jane Farley)

Stanley Leibo in Rome, October 2013, at the official dinner of the annual EMMA-JAX cryopreservation course in Monterotondo, Italy (Photograph kindly provided by Jane Farley)

It is with great sadness that we note the passing of Stanley Leibo, who died this morning, March 25, in the USA. Stanley Leibo was Professor at the Department of Biological Sciences, University of New Orleans, LA, USA. On behalf of the ISTT, we wish to offer our most sincere condolences to his family and friends.

Stanley Leibo was a renowned authority in cryobiology and one of the pioneers in the cryopreservation of mammalian germplasm. His seminal publication in Science in 1972, in collaboration with Peter Mazur and David Whittingham, described the first, successful cryopreservation of mammalian embryos, demonstrating the recovery of live pups from mouse embryos that had been stored in liquid nitrogen and liquid helium. His scientific work laid the foundation for the current widespread use of cryopreservation by human fertility clinics, agriculture, scientific resource repositories, and conservation efforts preserving endangered species. In addition to his outstanding scientific contributions, Stanley Leibo will be remembered for the joy and passion he brought to teaching. He was a fixture from the beginning in the Jackson Laboratory’s Cryopreservation Course, offered both in Bar Harbor and later also in partnership with EMMA in Monterotondo, Italy, where over the years hundreds of students, including many ISTT members,  had the pleasure of hearing his enthusiastic lectures on the history, theory and practice of cryopreserving mammalian cells and germplasm.

Stanley Leibo was a mentor for many of us, a colleague, and most of all, also a friend. We will not forget him. May he rest in peace.

ISTT council

P.S. Additional information from Stanley Leibo’s career and achievements can be obtained from this document, published in Reproduction, Fertiliy and Development, when he received the 2009 IETS Pioneer Award

Stanley Leibo with Marcello Raspa, in Monterotondo, November 2009, at the 10th anniversary of the EMMA-JAX annual cryopreservation course (Photograph by Lluis Montoliu).

Stanley Leibo with Marcello Raspa, in Monterotondo, November 2009, at the 10th anniversary of the EMMA-JAX annual cryopreservation course (Photograph by Lluis Montoliu).

Stanley Leibo with Martin Fray, at the 10th annual EMMA-JAX cryopreservation course in Monterotondo, Rome, Italy, in November 2009 (Photograph by Lluis Montoliu)

Stanley Leibo with Martin Fray, at the 10th annual EMMA-JAX cryopreservation course in Monterotondo, Rome, Italy, in November 2009 (Photograph by Lluis Montoliu)

Stanley Leibo's handwriting and drawings illustrating his passion for teaching. These were made on the paper tablecloth by himself, while explaining several concepts in cryobiology to other colleagues, during the official dinner of the last annual EMMA-JAX cryopreservation course, held in October 2013, in a restaurant in Rome (Photograph by Lluis Montoliu)

Stanley Leibo’s handwriting and drawings illustrating his passion for teaching. These were made on the paper tablecloth by himself, while explaining several concepts in cryobiology to other colleagues, during the official dinner of the last annual EMMA-JAX cryopreservation course, held in October 2013, in a restaurant in Rome (Photograph by Lluis Montoliu).

Stanley Leibo's lecture at the 50th anniversary of the Society for Cryobiology's Annual Meeting, in Washington, DC, USA, in July 2013. http://www.societyforcryobiology.org/cryo-2013

Stanley Leibo’s lecture at the 50th anniversary of the Society for Cryobiology’s Annual Meeting, in Washington, DC, USA, in July 2013. http://www.societyforcryobiology.org/cryo-2013

EMMA Cryopreservation Workshop in Madrid: a meeting report

Friday, May 11th, 2012
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EMMA Cryopreservation Workshop: a meeting report

EMMA Cryopreservation Workshop: a meeting report

The EMMA (European Mouse Mutant Archive) Cryopreservation Workshop took place earlier this week in Madrid, May 7-8, at the main campus of CSIC, with an excellent success, organized by EMMA, supported by the EC-7th Framework Programme and co-sponsored by the International Society for Transgenic Technologies (ISTT). Sixty participants from many countries around the world gathered to present and discuss, in depth, the latest approaches, methodologies and techniques available for the efficient cryopreservation of mouse strains, through embryo, sperm and ovary cryopreservation. In addition to invited speakers and invited participants, the workshop was attended by delegates from EMMA nodes and 12 ISTT members. As many as 21 talks were delivered, by selected invited speakers, representing the different major archiving iniatives currently existing (EMMA, MMRRC, The Jackson Laboratory, RIKEN, APN, CMMR, AMMRA) and the research and development initiatives, as well as state-of-art protocols in the field. Presentations, abstracts and pictures from this EMMA Cryopreservation Workshop are freely available to anyone interested, from the meeting web site, and can be also accessed from ISTT and EMMA web sites. The remaining presentations will be progressively added upon receiving the approval from the corresponding guest speakers.

At EMMA, we envisaged this cryopreservation workshop as a forum to brainstorm and discuss in depth the latest technological advances in cryopreservation, including sperm and embryo cryopreservation, updated IVF methods and related techniques as ovary cryopreservation, laser-assisted and piezo-driven ICSI, transportation of frozen material and other technical and logistic challenges relevant to the operation of current mouse embryo/sperm archives. We believed we entirely fulfilled the expectations and all participants went back home, to their research institutions, loaded with new ideas, updated solutions and suggested improvements that can be explored and applied for a most efficient management of a mouse embryo/sperm cryopreservation bank. All participants agreed to continue organizing this type of focused workshops in the near future. The ISTT will be always there, ready to support these very interesting initiatives.

Lluis Montoliu, EMMA Spanish node

EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

Thursday, February 16th, 2012
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EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

The International Society for Transgenic Technologies (ISTT) co-sponsors the EMMA Cryopreservation Workshop, organized by EMMA (the European Mouse Mutant Archive) and CSIC (the Spanish Research Council) and to be held at the CSIC Main Campus, in Madrid (Spain), on 7-8 May 2012. The Organizers of this workshop are Martin Fray (Mammalian Genetics Unit, MRC, Harwell, UK; Biological Resources Manager at the EMMA node in MRC-MGU-Harwell and ISTT Member), Michael Hagn (Institute of Experimental Genetics, HMGU, Munich, Germany; EMMA Project Manager) and Lluis Montoliu (National Center of Biotechnology, CNB-CSIC, Madrid, Spain; Spanish EMMA node co-ordinator and ISTT Member).  The Organizers have selected a number of speakers in the field and hot topics in cryopreservation with a main focus on technology. The main aim of this workshop is to discuss openly all topics at length and in depth, from past and present initiatives, undertaken by the various archiving initiatives world-wide, invited to this workshop, to the current and future challenges all bio-repositories have to face and address adequately. Topics that will be discussed include: embryo and organ cryopreservation, sperm cryopreservation and IVF, ICSI, mouse production, morula/ES aggregation method, transportation issues with frozen and unfrozen biological material and various continental efforts towards cryopreservation. Additional information can be obtained from workshop web site.

Confirmed invited participants include:

  • Sue Bath (Melbourne, Australia)
  • Martina Crispo (Institut Pasteur, Montevideo, Uruguay)
  • Xiang Gao (Model Animal Research Center, Nanjing University, Nanjing, P.R. China)
  • Marina Gertsenstein (Toronto Centre for Phenogenomics, Toronto, ON, Canada)
  • Alan Hart (MRC-Human Genetics Unit at MRC/IGMM, University of Edinburgh, Edinburgh, UK)
  • Jean Jaubert (Institut Pasteur, Paris, France)
  • Carlisle Landel (Thomas Jefferson University, Kimmel Cancer Center, Philadelphia, PA, USA)
  • Kent Lloyd (Mouse Biology Program, University of California, Davis, CA, USA)
  • Peter Mazur (The University of Tennessee, Knoxville, TN, USA)
  • Keiji Mochida (RIKEN Bioresource Center, Tsukuba-shi, Ibaraki, Japan)
  • Pedro Moreira (European Molecular Biology Laboratory, Monterotondo/Rome, Italy)
  • Naomi Nakagata (Center for Animal Resources & Development-CARD, Kumamoto University, Kumamoto, Japan)
  • Lauryl Nutter (Toronto Centre for Phenogenomics, Toronto, ON, Canada)
  • Atsuo Ogura (RIKEN Bioresource Center, Tsukuba-shi, Ibaraki, Japan)
  • Sagrario Ortega (National Cancer Research Center, CNIO-ISCIII, Madrid, Spain)
  • Belén Pintado (National Center of Biotechnology, CNB-CSIC, Madrid, Spain)
  • Marcello Raspa (EMMA-CNR, Monterotondo/Rome, Italy)
  • Stuart Read (The Australian National University, The Australian Phenomics Facility, Canberra, Australia)
  • Jorge Sztein (CMB Cryopreservation and Assisted Reproduction, NIAID-NIH, Rockville, MD, USA)
  • Rob Taft (The Jackson Laboratory, Bar Arbor, ME, USA)
  • Toru Takeo (Center for Animal Resources & Development-CARD, Kumamoto University, Kumamoto, Japan)
  • Xavier Warot (Center of PhenoGenomics, School of Life Sciences, EPFL, Lausanne, Switzerland)
  • Michael Wiles (The Jackson Laboratory, Bar Arbor, ME, USA)

In addition, the EMMA Cryopreservation workshop will be attended by delegates from EMMA nodes and up to ten (10) current* ISTT members selected among applicants with expertise in cryopreservation, on behalf of the EMMA-ISTT cooperation agreement, for mutual promotion and collaboration, currently in place, and thanks to the specific co-sponsorship of the ISTT agreed by the ISTT council for this EMMA workshop. This is a highly specialized workshop with about 60 participants, all attending by invitation. However, up to ten ISTT members with expertise in cryopreservation will be able to benefit and attend. ISTT members interested and willing to attend this meeting can submit their applications to Lluis Montoliu (montoliu@cnb.csic.es), including a CV and a letter describing how the applicant will benefit from attending the workshop, but also how the applicant will be able to contribute to discussions. Applications should be sent by email to Lluis Montoliu by February 29th, 2012. Thereafter, Organizers will review all received applications and select up to ten ISTT Members with expertise in cryopreservation. All invited participants will be encouraged to actively take part in all discussions throughout the meeting. There is no registration fee for this workshop. Selected ISTT members will be entitled to one night accommodation (7 to 8 May 2012) on behalf of the Organization of this workshop. All participants will be invited to the official Workshop dinner on May 7.

Also, in an effort to disseminate the outcome of this workshop among interested colleagues, the Organizers will make the workshop abstracts, submitted by invited speakers, and some/all of speakers’ slides available on the public EMMA and ISTT web sites, after obtaining the specific permission to share material from each invited speaker.

(*) current ISTT members are those registered/renewed in 2012.

New videos on cryopreservation procedures added to ISTT collection

Wednesday, May 4th, 2011
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New videos on cryopreservation procedures added to ISTT collection

New videos on cryopreservation procedures added to ISTT collection

Three new videos on basic cryopreservation techniques have been added to the ISTT collection, available to ISTT members, within the members-only area of the ISTT site. These videos, prepared by Julia Fernández, María Jesús del Hierro and Lluís Montoliu, from the CNB-CSIC, Spanish EMMA node, in Madrid, illustrate the correct access to manually- and automatically-filled liquid nitrogen tanks, depicting standard safety measures to be taken into account when operating in a room with liquid nitrogen tanks.

The safety measures illustrated in these videos include: double checking the low-oxygen content red-light alarm (oxygen monitors) before entering the room with the liquid nitrogen tanks, never working alone (at least two people are always visiting the liquid nitrogen containers), protecting your face and eyes with an appropriate plastic shield or glasses, protecting your hands with adequate gloves and, in addition, working fast and without interruptions, thus avoiding to hold the racks or cylinders with cryopreserved samples for an unnecessary amount of time outside the liquid nitrogen.

Be always very careful when working with liquid nitrogen and always pay attention to safety rules. Liquid nitrogen can cause severe frostbite and cold burns. Always handle liquid nitrogen in well-ventilated areas to prevent excessive concentrations of gas that will rapidly displace oxygen and kill people by asphyxiation.

The following list contains some web sites with additional information on SAFETY MEASURES FOR HANDLING LIQUID NITROGEN:

2011 Cryopreservation Course EMMA-JAX, Monterotondo, Italy, October 17-21

Thursday, March 24th, 2011
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2011 Cryopreservation Course EMMA-JAX, Monterotondo, Italy, October 17-21

2011 Cryopreservation Course EMMA-JAX, Monterotondo, Italy, October 17-21

The Consiglio Nazionale delle Ricerche – “A. Buzzati-Traverso” Campus and The Jackson Laboratory offer the 2011 edition of a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. This Cryopreservation Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA). The Course will be held in Monterotondo, Italy, on October 17-21, 2011.

The course is offered to teach methods in cryopreservation for banking of various strains of mice used in research, including inbred, transgenic and knock-out strains. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a “hands-on” laboratory program in which participants learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries.

Course faculties include: Stanley P. Leibo, Robert Taft, Jane Farley, Sian Clements, Marcello Raspa, Ferdinando Scavizzi, Raffaele Matteoni, Susan Marschall, Martin Fray and K.C. Kent Lloyd. Enrollment is limited to 10 participants and early application is advised. Deadline for applications: September 20, 2011.

Flyer with information about the 2011 EMMA-JAX Cryopreservation Course

Application form

Course Information email address: cryocourse@ibc.cnr.it

EMMA-JAX cryocourse 2010

Wednesday, May 5th, 2010
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EMMA-JAX cryopreservation course 2010

EMMA-JAX cryopreservation course 2010

The Istituto di Biologia Cellulare of Consiglio Nazionale delle Ricerche-”A. Buzzati-Traverso” Campus and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. This Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA). The course will be held at the “A. Buzzatti-Traverso” Campus, in Monterotondo, Rome, Italy on October 25-29, 2010.

The course is offered to teach methods in cryopreservation for banking of various strains of mice used in research, including inbred, transgenic and knock-out strains. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a “hands-on” laboratory program in which participants learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries.

Additional information about this EMMA-JAX Cryocourse 2010

Application form

Deadline for submitting applications: September 25, 2010

10th Anniversary EMMA-JAX cryopreservation course

Thursday, November 26th, 2009
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10th anniversary EMMA-JAX cryopreservation course (Marcello Raspa, left, and Stanley Leybo)

10th anniversary EMMA-JAX cryopreservation course (Marcello Raspa, left, and Stanley Leybo)

The 10th anniversary EMMA-JAX cryopreservation course has been held in Monterotondo-Rome (Italy), at the IBC-CNR “A. Buzzati-Traverso” Campus, on November 23-26, 2009. ISTT has supported this course, according to the cooperation agreement signed with EMMA, and hence, the Society was presented to all participants and teachers. Ten participants were selected among all applications received and could learn about mouse embryo and sperm cryopreservation, among other related topics. They practised state-of-the-art techniques about how to cryopreserve mouse sperm, how to do an in vitro fertilization, how to obtain and cryopreserve 2-cell IVF-derived mouse embryos and how to do vitrification. In these ten editions since 1999, more than hundred participants have attended these annual cryopreservation courses, thus contributing to the successful spreading of these techniques among many laboratories from many countries around the world.

IBC-CNR, EMMA and JAX logos

IBC-CNR, EMMA and JAX logos

 The faculties for this course were Stanley Leibo (Univ. New Orleans, LA, USA), as Course Director; Robert Taft (The Jackson Laboratory, Bar Harbor, ME, USA); Jorge Sztein (NIAID-NIH, Rockwille, MD, USA), Kent Lloyd (Univ. California, Davis, CA, USA), Martin Fray (MRC-MGU, Harwell, Didcot, UK), Susan Marschall (IEG, Munich, Germany); Lluis Montoliu (CNB-CSIC, Madrid, Spain); and Raffaele Matteoni, Ferdinando Scavizzi and Marcello Raspa, from IBC-CNR-EMMA, Monterotondo, Italy. The instructors included Jane Farley and Sian Clemmens, from The Jackson Laboratory, and Arsenio Armagno, Daniele Iannilli, Tiziana La Penna, Ilaria Losso and the rest of cryopreservation support personnel from IBC-CNR-EMMA, Monterotondo, Italy.

10th anniversary EMMA-JAX cryopreservation course

10th anniversary EMMA-JAX cryopreservation course

EMMA Laboratory Course on Cryopreservation of Mouse Germplasm

Tuesday, July 21st, 2009
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EMMA Monterotondo Cryopreservation Course 2009

EMMA Monterotondo Cryopreservation Course 2009

 The Istituto di Biologia Cellulare of Consiglio Nazionale delle Ricerche-”A. Buzzati-Traverso” Campus and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries that will be held in Monterotondo (Italy), on November 23-26, 2009. This Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA).

Deadline for submitting applications: October 15, 2009

Additional information about this cryopreservation course: INFORMATION

Course application form: APPLICATION FORM

Enrollment is limited to 10 participants and early application is advised.


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