Archive for September, 2011

The tg-list will be transferred to the ISTT web server

Friday, September 30th, 2011
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The tg-list will be transferred to the ISTT web server

The tg-list will be transferred to the ISTT web server

The International Society for Transgenic Technologies (ISTT) is pleased to announce that the transgenic-list (tg-l) owner and administrator, Dr. Peter Sobieszczuk and the ISTT have just signed an agreement to transfer the tg-l from its current location, at the web server of the Imperial College (London, UK), to the ISTT web server.

The tg-l was created by Peter Sobieszczuk in July, 1996, to serve the global research community specializing in genetic modifications of experimental laboratory animals. Since then, the tg-l has been hosted by three academic institutions: the IGBMC in Strasbourg, the University of Manchester, UK, and the Imperial College in London, UK where it is currently hosted. The ISTT would like to acknowledge the excellent work of Peter and his assistants in maintaining the list since its inception for the benefit of everyone who has been a part of this list. The tg-l has proven to be a valuable source of knowledge and advice, helping many newcomers to rapidly familiarize themselves with the field of animal transgenesis, and facilitating the exchange of protocols and experiences among all of its members.

The ISTT, in accordance with one of its main aims, as expressed in the ISTT bylaws (Art. 3a), is most proud to host the tg-l for the benefit of the entire community of scientists, technicians, students and anyone else, in general, interested in animal transgenesis.

At the ISTT server, the tg-l will continue being public (anyone may subscribe, including ISTT members and non-members alike), unmoderated (messages will not be altered by the list administrator) and closed (only subscribers may post messages). Subscription to the tg-l will also continue being free, automatic and very simple, from a suitable page that will be installed within the public area of the ISTT web server.

The actual transfer of tg-l from the Imperial College to the ISTT web server will not occur until current agreements in place will be fulfilled. This is expected to occur by December 31, 2011 or, earlier, whenever possible. The actual transfer will be duly informed in subsequent messages that will be posted through the ISTT_list and through the tg-l.

best regards,

Lluis Montoliu
President of the ISTT

Dr. Ralph L. Brinster receives US National Medal of Science

Wednesday, September 28th, 2011
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Dr. Ralph L. Brinster receives US National Medal of Science

Dr. Ralph L. Brinster receives US National Medal of Science

US President Obama named today Dr. Ralph L. Brinster (University of Pennsylvania) as recipient of the US National Medal of ScienceFor his fundamental contributions to the development and use of transgenic mice.  His research has provided experimental foundations and inspiration for progress in germline genetic modification in a range of species, which has generated a revolution in biology, medicine, and agriculture“.  The ISTT wishes to congratulate Dr. Ralph L. Brinster for this prestigious award. Here you can read the University of Pennsylvania Press release.

Dr. Ralph L. Brinster receives this US National Medal of Science along with six other eminent researchers: Jacqueline K. Barton (California Institute of Technology), Shu Chien (University of California, San Diego), Rudolf Jaenisch (Whitehead Institute for Biomedical Research and Massachusetts Institute of Technology), Peter J. Stang (University of Utah), Richard A. Tapia (Rice University) and Srinivasa S.R. Varadhan (New York University).

Dr. Ralph L. Brinster has received numerous awards in his career. The International Society for Transgenic Technologies (ISTT) will be awarding him the ISTT Prize “for his outstanding contributions to the field of transgenic technologies” during the forthcoming 10th Transgenic Technology (TT2011) meeting, to be held in St Pete Beach, Florida n October 24-26, 2011.

ISTT is a new AAALAC’s Member Organization

Tuesday, September 27th, 2011
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ISTT is a new AAALAC's Member Organization

ISTT is a new AAALAC's Member Organization

The International Society for Transgenic Technologies (ISTT) is pleased to announce that has been approved as a new AAALAC’s Member Organization after our application we registered in July, with the massive support of ISTT members. AAALAC stands for the “Association for Assessment and Accreditation of Laboratory Animal Care”. AAALAC International is a private, nonprofit organization that promotes the humane treatment of animals in science through voluntary accreditation and assessment programs. AAALAC’s Member Organizations are a select group of prestigious scientific, professional and educational groups with an interest in advancing biomedical research and animal well-being in science. Each Member Organization appoints a representative to serve on the AAALAC International Board of Trustees.

Genome sequencing of 17 mouse inbred strains

Friday, September 16th, 2011
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Mouse genomic variation and its effect on phenotypes and gene regulation. Keane et al. Nature 2011, 289–294,  (15 September 2011), doi:10.1038/nature10413

Mouse genomic variation and its effect on phenotypes and gene regulation. Keane et al. Nature 2011, 289–294, (15 September 2011), doi:10.1038/nature10413

Most of our current understanding and genome research in mice has been based, so far, on the genome of the mouse inbred strain C57BL/6J, published in 2002 and accessible through a number of genome browsers, such as Ensembl. Yesterday, Keane et al. published in Nature the results of their sequencing efforts and the release of the genome sequences from 17 additional mouse inbred strains: C3H/HeJ, CBA/J, A/J, AKR/J, DBA/2J, LP/J, BALB/cJ, NZO/HlLtJ, NOD/ShiLtJ, 129S5SvEv<Brd>, 129P2/OlaHsd, 129S1/SvImJ, C57BL/6NJ, and the four wild-derived inbred strains CAST/EiJ, PWK/PhJ, WSB/EiJ and SPRET/EiJ. This is an enormous amount of information, obtained using next-generation sequencing, that these researchers from The Wellcome Trust Sanger Institute and other collaborating institutions now release to the public scientific community. Most of these inbred strains are very relevant in biomedical research and they are regularly used for the generation of genetically-modified mice. Notably, among them the C57BL/6NJ inbred strain, selected by the International KnockOut Mouse Consortium (IKMC) to produce the collection of systematic gene knockouts covering the entire mouse genome. This publication and the subsequent analyses derived from this study should greatly contribute to our understanding of the genetic diversity among different mouse strains and the different phenotypes that are often observed across inbred strains, associated with identical genetic modifications.

I International Scientific Meeting for the Optimization of Murine Models in Research, Valdivia, Chile, 24-26 January 2012

Wednesday, September 14th, 2011
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I International Scientific Meeting for the Optimization of Murine Models in Research, Valdivia, Chile, 24-26 January 2012

I International Scientific Meeting for the Optimization of Murine Models in Research, Valdivia, Chile, 24-26 January 2012

The International Society for Transgenic Technologies (ISTT), is pleased to announce the co-sponsorship of the I International Scientific Meeting for the Optimization of Murine Models in Research, that will be held at the Centro de Estudios Cientificos (CECs), in Valdivia, Chile, on 24-26 January 2012. This meeting is organized by Juan Manuel Baamonde (CECs, Chair), and the ISTT Members Fernando Benavides (MD Anderson CC, Smithville, TX, USA) and Jorge Sztein (NIH/NIAID, USA), along other CECs scientists. The meeting will be followed by the III Practical Course CECs: Mouse sperm freezing according to JAX method, coordinated by Dr. Jorge Sztein, on 27 January 2012. Talks will be given in Spanish, Portughese or English. ISTT members are entitled to a reduced registration fee, both for the meeting and the course. The practical course is limited to 15 participants. Early bird registration deadline is October 15, 2011.

Advanced Protocols for Animal Transgenesis. An ISTT Manual

Friday, September 9th, 2011
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Advanced Protocols for Animal Transgenesis. An ISTT Manual

Advanced Protocols for Animal Transgenesis. An ISTT Manual

The International Society for Transgenic Technologies (ISTT) is most pleased to announce the publication of its first book, entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thom Saunders, and published by Springer (2011), in collaboration with the ISTT. This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists, most of them members of the ISTT, who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement. This book comprises 26 chapters,  109 illlustrations and 669 pages.

Table of contents, chapters of this ISTT Manual:

  • 1 Patent and Licensing Issues in Transgenic Technology. Karen S. Canady
  • 2 Global Resources: Including Gene Trapped ES Cell. Clones – Is Your Gene Already Knocked Out?. Lluis Montoliu
  • 3 Designing Transgenes for Optimal Expression. Eduardo Moltó, Cristina Vicente-García, and Lluis Montoliu
  • 4 Gene Targeting Vector Design for Embryonic Stem Cell Modifications. Thomas L. Saunders
  • 5 Transgenic Production Benchmarks. Thomas J. Fielder and Lluis Montoliu
  • 6 Generation of Transgenic Mice by Pronuclear Microinjection. Katja Becker and Boris Jerchow
  • 7 Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, and Ignacio Anegon
  • 8 Generation of Transgenic Animals by Use of YACs. Almudena Fernández, Diego Muñoz, and Lluis Montoliu
  • 9 BAC Transgenes, DNA Purification, and Transgenic Mouse Production. Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
  • 10 Generation of Transgenic Animals with Lentiviral Vectors. Carlos Lois
  • 11 Vertebrate Transgenesis by Transposition. Aron Geurts, Darius Balciunas, and Lajos Mates
  • 12 Rat Spermatogonial Stem Cell-Mediated Gene Transfer. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, and F. Kent Hamra
  • 13 Mouse Cloning by Nuclear Transfer. Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
  • 14 Gene Targeting in Embryonic Stem Cells. Elizabeth D. Hughes and Thomas L. Saunders
  • 15 The Importance of Mouse ES Cell Line Selection. Wojtek Auerbach and Anna B. Auerbach
  • 16 Tetraploid Complementation Assay. Marina Gertsenstein
  • 17 Combining ES Cells with Embryos. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, and Marina Gertsenstein
  • 18 Derivation of Murine ES Cell Lines. Kristina Nagy and Jennifer Nichols
  • 19 Rat Embryonic Stem Cell Derivation and Propagation. Ping Li, Eric N. Schulze, Chang Tong, and Qi-Long Ying
  • 20 Induced Pluripotency: Generation of iPS Cells from Mouse Embryonic Fibroblasts. Han Li, Katerina Strati, Verónica Dom?ínguez, Javier Martín, María Blasco, Manuel Serrano, and Sagrario Ortega
  • 21 The Preparation and Analysis of DNA for Use in Transgenic Technology. Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
  • 22 Colony Management. Karen Brennan
  • 23 Cryopreservation. Belén Pintado and Juan de Dios Hourcade
  • 24 Shipment of Mice and Embryos. Shirley Pease
  • 25 Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy. Jorge M. Sztein, R.J. Kastenmayer, and K.A. Perdue
  • 26 Refinement, Reduction, and Replacement. Jan Parker-Thornburg

ISTT Members are entitled to purchase this ISTT Manual with a 33% discount, through the members-only area of the web site.

TT2011 meeting: early bird registration up to September 15

Thursday, September 1st, 2011
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TT2011 meeting, 24-26 October 2011, Florida, USA

TT2011 meeting, 24-26 October 2011, Florida, USA

Have you not yet registered for the TT2011 meeting? Registration at Early Bird reduced fees are still accepted up to September 15th. Moreover, if you are not a member of the ISTT this is also a perfect time to become a member, since ISTT members will further benefit of a reduced registration fee until October 9th. Please tell your colleagues within our field and inform them about this meeting, in case they are not aware of it. Don’t miss this opportunity to discuss the latest advances in animal transgenesis technologies with colleagues and friends from all over the world. The list of speakers invited for the TT2011 meeting is very attractive and ensures high level and most interesting discussions for the topics considered at this conference.  The TT2011 meeting will be held at TradeWinds Island Grand Resort, St. Pete Beach, Florida, USA, on October 24-26, 2011. All information about this meeting is available in its dedicated web site: www.tt2011.org and questions, comments and queries can be sent to its dedicated email address: tt2011@transtechsociety.org

See you all in Florida!


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