Marina Gertsenstein (member of ISTT) and her colleagues, from the Samuel Lunenfeld Research Institute, Mount Sinai Hospital and the Toronto Centre for Phenogenomics, in Toronto (ON, Canada), have recently published a scientific article in PLoS ONE describing the efficient generation of mouse chimeras with C57BL/6N ES cells by aggregation with standard albino outbred ICR morulas. As detailed in this work, the use of chemically-defined ES cell culture medium (2i) appears to be crucial for the success of the experiment. This is an interesting development that can help the biomedical research community for the easy and rapid generation of C57BL/6N -derived chimeras at a reduced cost. It also complements the toolbox by which chimeras can be obtained with the popular C57BL/6N ES cells, used in the large-scale international consortiums (i.e. IKMC) aiming to generate systematic knock-outs of all genes in the mouse genome.
Archive for July, 2010
B6N ES cells can be aggregated with albino outbred ICR morulas for the efficient production of chimerasFriday, July 16th, 2010
The International Society for Transgenic Technologies (ISTT) has decided to co-sponsor the QTRN’s 6th Annual Symposium: Focus on new transgenic models and their phenotyping, organized by the Quebec Transgenic Research Network (QTRN), that will be held in Montreal, Quebec, Canada, on November 13, 2010.
This symposium will be co-organized by Jacinthe Sirois. ISTT already co-sponsored the most successful QTRN’s 5th Annual Symposium in 2009, devoted to Transgenesis: new developments and applications. Additional information will be provided in the symposium web site at due time.