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7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

Tuesday, February 12th, 2013
7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the 7th Workshop on Innovative Mouse Models (IMM2013), which will be held at the Leiden University Medical Center (LUMC), in Leiden, The Netherlands, on 13-14 June 2013.  This will be the third consecutive edition of this meeting series that is granted with the co-sponsorship of the ISTT, after the IMM2011 and the IMM2009 editions, due to its interest and relevance for the community of researchers using genetically-modified mice. The IMM2013 workshop is organized by: Jos Jonkers (NKI-AVL, Amsterdam, The Netherlands), Paul Krimpenfort (NKI-AVL, Amsterdam, The Netherlands), Werner Mueller (University of Manchester, Manchester, United Kingdom), Hein te Riele (NKI-AVL, Amsterdam, The Netherlands), Els Robanus-Maandag (LUMC, Leiden, The Netherlands), Marian van Roon (VU, Amsterdam, The Netherlands and ISTT Member) and Sjef Verbeek (LUMC, Leiden, The Netherlands and ISTT Member).

According to the IMM2013 workshop web page: The primary goal of this two-day workshop is to bring together a diverse group of scientists interested in advanced genome alteration approaches in the mouse, including key developers of emerging technologies as well as researchers who wish to apply and assess these new approaches. The IMM2013 workshop will encourage an in-depth and unvarnished discussion of these technologies and novel developments. This 2-day workshop will have a mixture of invited speakers and selected presentations. Keynote lectures will be given by:

  • Anton Wutz (UK) haploid ESC
  • Haoyi Wang (US) iPS / TALENs
  • Bill Skarnes (UK) high throughput TALENs
  • Ben Davis (UK) docking site/ new recombinases
  • Yann Herault (France) Cre transgenic mice
  • Kevin Brindle (UK) MRI
  • Mathijs Verhagen (NL) large scale phenotyping
  • B Kappes (US) TALENs
  • Zoltan Ivics (Germany) Transposons
Registration will open soon. ISTT members will be entitled to a reduced registration fee.

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John B. Gurdon and Shinya Yamanaka awarded the 2012 Nobel Prize in Physiology or Medicine

Monday, October 8th, 2012
John B. Gurdon (left) and Shinya Yamanaka (right) awarded the 2012 Nobel Prize in Physiology or Medicine

John B. Gurdon (left) and Shinya Yamanaka (right) awarded the 2012 Nobel Prize in Physiology or Medicine

The Nobel Prize in Physiology or Medicine 2012 was awarded today, October 8, 2012, jointly to Sir John B. Gurdon (Gurdon Institute, Cambridge, United Kingdom) and Shinya Yamanaka ( Kyoto University, Kyoto, Japan, and Gladstone Institute, San Francisco, CA, USA) “for the discovery that mature cells can be reprogrammed to become pluripotent“. The Nobel Assembly at Karolinska Institutet (Stockholm, Sweden) decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to John B. Gurdon and Shinya Yamanaka. According to the published Press Release: “The Nobel Prize recognizes two scientists who discovered that mature, specialised cells can be reprogrammed to become immature cells capable of developing into all tissues of the body. Their findings have revolutionised our understanding of how cells and organisms develop“. The International Society for Transgenic Technologies (ISTT) wishes to congratulate both excellent scientists for their outstanding achievements and the very much deserved Nobel Prize they have just been awarded.

John B. Gurdon demonstrated in 1962 that adult frogs could be obtained by the transplantation of nuclei from endoderm cells of Xenopus laevis donors ranging from late blastulae to swimming tadpoles.  In 1966, John B. Gurdon continued his studies on cellular reprogramming in frogs and reported that fertile adult male and female frogs, genetically marked as of solely donor origin, had been obtained from the transplantation of nuclei from intestinal epithelial cells of Xenopus laevis feeding larvae. In 1975, John B. Gurdon reported that tadpoles could be obtained from nuclei transplanted from keratinized skin cells of adult frogs. However, no adult frogs were obtained from nuclear transfer experiments involving adult somatic cells. As concluded by Gurdon and Byrne in 2003, these first series of results obtained by John B. Gurdon, “established the general principle that the process of cell differentiation does not necessarily require any stable change to the genetic constitution of a cell. Thus, cell differentiation depends on changes in the expression not content of the genome“. Similar principles had been envisaged and proposed, but could not be proved, in 1938 by the German embryologist Hans Spemann (awarded the 1935 Nobel Prize in Physiology or Medicine).

Forty years later, after the outstanding results obtained by John B. Gurdon, Shinya Yamanaka reported in 2006 the identification of  four transcription factors (Oct3/4, Sox2, c-Myc, and Klf4) capable of reprogramming any somatic cell into a cell with properties of a pluripotent stem cell, similar to embryonic stem (ES) cells. Those cells were named as inducible pluripotent stem cell (or iPS cells) and greatly revolutionized the regenerative medicine field and cellular reprogramming studies since then. Yamanaka’s experiment provided a totally innovative method to obtain human pluripotent stem cells, with great regenerative potential, without requiring the use of human embryos, an achievement that has been greatly acknowledged by members of the society who did not accept, according to their private beliefs, the previous use of human embryos to obtain pluripotent stem cells. John B. Gurdon and Shinya Yamanaka had already been awarded jointly the 2009 Albert Lasker Basic Medical Research Prize “for discoveries concerning nuclear reprogramming, the process that instructs specialized adult cells to form early stem cells — creating the potential to become any type of mature cell for experimental or therapeutic purposes“.

The history of nuclear transplantation and cellular reprogramming has been silently advancing since the beginning of last century, and progressed through a series of phenomenal milestones that were regularly achieved. Starting with the pioneer experiments and the vision of Hans Spemann (1938), then the first nuclear transfer success in frogs by Briggs and King (1952), of course the experiments carried out by John B. Gurdon in the 60′s and early 70′s, already mentioned, and also the studies by Marie DiBerardino (1967) on the effect of cell cycle in the nuclear transfer success, and other, more recent, describing the role of single transcription factor being able to change the fate of a cell (Harold Weintraub, 1987) or the direct reprogramming experiments, betweeen different cellular haematopoietic types, pioneered by Thomas Graf since 1990. However, in my opinion, the one single study that, for the first time, demonstrated that a nucleus from an adult terminally and fully differentiated somatic cell (i.e. a cell derived from mammary gland tissue) could give rise to a fertile normal adult, closing the circle of life, was the birth of Dolly, the sheep, obtained by Ian Wilmut and his collaborators from the Roslin Institute in 1996 and reported in a famous paper in Nature in February 1997.

Ian Wilmut and Dolly the sheep in 1997

Ian Wilmut and Dolly the sheep in 1997

The impact of Wilmut’s study on Biology, Biomedicine, Biotechnology and on the entire Society was phenomenal, tremendous (unfortunately not always positive, independent of him, with intense and never ending debates beyond science) and triggered many subsequent studies, including the use of human pluripotent stem cells, isolated by Thomson and Gearhart teams in 1998, and their potential in regenerative medicine, and eventually the work by Yamanaka and many other. The Nobel Assembly has limited this Nobel award to Gurdon and Yamanaka, who fully deserved the Nobel Prize, but, unfortunately, they did not include other scientists that were instrumental for the progress in reprogramming studies, particularly Ian Wilmut. Dolly’s experiment  is only referred briefly in the advance information provided to interested readers. After discussing this issue the whole day with many colleagues, I believe I speak on behalf of many by stating that a Nobel Prize awarded to Gurdon, Wilmut and Yamanaka, could have been a much better and balanced choice. Gurdon himself stated that he would have liked to share this Prize with Ian Wilmut.  But this is just wishful thinking and the reality is, as usual, different. Having said that, I want to congratulate once again John B. Gurdon and Shinya Yamanaka for this Nobel Prize.

On a final sad note, after all the excitement associated with this Nobel Award to reprogramming techniques, we have been informed of the sudden death of Keith H. Campbell, biologist (1954 – 2012), who passed away last Saturday, October 6, 2012. Keith H. Campbell was one of the main co-authors, together with Ian Wilmut, of Dolly’s Nature paper in 1997 (Viable offspring derived from fetal and adult mammalian cells. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH, Nature 1997 Feb 27;385(6619):810-3). Keith H. Campbell was currently working at the School of Biosciences, University of Nottingham, UK.

 

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The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

Sunday, September 23rd, 2012
The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The International Society for Transgenic Technologies (ISTT) was founded shortly after the Transgenic Technology (TT) meeting in Barcelona (TT2005). Since then, the ISTT family has been fortunate to visit several countries, every ~18 months. In 2007 we went to Brisbane (TT2007).  In 2008, we visited Toronto (TT2008). In 2010 we returned to Europe and held the TT2010 meeting in Berlin. For 2011 we visited the USA for the first time, and organized the TT2011 meeting in St Pete Beach (Florida). And, now, the next TT meeting (TT2013) is planned for China, in Guangzhou. This has been one of our aims and challenges, since the foundation of the ISTT, namely, holding a TT meeting in Asia, in China. Now it has become a reality. The 11th Transgenic Technology meeting (TT2013) will be held in Guangzhou (China), on 25-27 February 2013, organized by Prof. Ming Zhao (Chair) (Southern Medical University, Guangzhou) and his Organizing and Advisory Committees, immediately after celebrating the Chinese New Year of the Snake. More than 30 speakers have confirmed their participation, to discuss about ES cells, iPS cells, targeted nucleases (ZFNs and TALENs), cryopreservation and reproduction techniques, running a transgenic facility, mouse genetics, epigenetics, ethics and animal welfare, transgenesis in other vertebrates, animal models of disease, etc… among many other interesting topics. At the TT2013 meeting, the ISTT will award the 9th ISTT Prize for outstanding contributions to transgenic technologies to Prof. Allan Bradley,  Director Emeritus of the Wellcome Trust Sanger Institute (WTSI), in Hinxton (UK), and leader of the Mouse Genomics Team at WTSI.

In addition, a 3-day hands-on practical workshop (28 February-2 March 2013) will be offered in Guangzhou after the TT2013 meeting, addressing basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management and other interesting topics. This workshop is organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair,  Ming Zhao (Southern Medical University, Guangzhou, China), Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China).

Don’t miss this opportunity to hear the latest advances in animal transgenic by world experts! Time is going fast and first deadlines (15 October 2012), for registering at reduced fees, for submitting abstracts, for applying for ISTT registration awards and for nominating candidates for ISTT Young Investigator awards are rapidly approaching.

See you all in China!

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Registration for the TT2013 meeting in China is OPEN

Thursday, June 7th, 2012
Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

On behalf of the Organizing Committee for the 11th Transgenic Technology (TT2013) meeting and of the International Society for Transgenic Technologies (ISTT) it is my great pleasure to announce the launching of the official TT2013 meeting web site and, at the same time, inform that registration for the TT2013 meeting is now OPEN. After a very successful TT2011 meeting in Florida, the ISTT family travels to far-East, to China, to hold the 11th Transgenic Technology meeting in the city of Guangzhou, the largest city of the Guandong province in Southern China, on February 25-27, 2013. The TT2013 meeting is locally organized by the Southern Medical University, in Guangzhou, and the Chair of the Organizing Committee is Prof. Ming Zhao.

TT2013 meeting web site: http://www.tt2013.org

TT2013 meeting official email address: tt2013@transtechsociety.org

We welcome all attendees who are in the process of generating genetically-modified animals or who perform the experiments that follow to determine the corresponding phenotypes of such transgenic animals. The program should be of interest to scientists, group leaders, postdoctoral researchers, facility managers, technicians and PhD students working directly or indirectly with genetically-modified animals. We invite you to participate and contribute to this conference where we will discuss the latest technology developments, the newest applications and strategies in biology, biomedicine and biotechnology using transgenic animals. Companies and institutions with an interest in this field are kindly invited to participate and share their latest technologies. We encourage you to submit abstracts to be presented at the TT2013 meeting.

We have confirmed a wonderful list of excellent speakers and interesting topics for the animal transgenesis community. The list of invited speakers confirmed to attend the TT2013 meeting includes:

  • Fernando Benavides (MD Anderson Cancer Center, Smithville, TX, USA)
  • Allan Bradley (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK) ISTT Prize
  • James Bussell (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK)
  • Shannon Byers (The Jackson Laboratory, Bar Harbor, ME, USA)
  • Toni Cathomen (University Medical Center Freiburg, Germany)
  • Alan Colman (Institute of Medical Biology, Singapore) Closing Talk
  • Michael Dobbie (Australian Phenomics Facility, The Australian National University, Canberra, Australia)
  • Scott Fahrenkrug (Recombinetics, Minneapolis, MN, USA)
  • Malcolm France (Sydney University, Sydney, Australia)
  • Xiang Gao (Model Animal Research Center of Nanjing University, Nanjing, PR China)
  • Alfonso Gutiérrez-Adán (Dep. Animal Reproduction, INIA, Madrid, Spain)
  • Yann Herault (Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France)
  • Benoît Kanzler (Max-Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany)
  • Dietmar Kappes (Fox Chase Cancer Center, Philadelphia, PA, USA)
  • Takashi Kohda (Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan)
  • Thomas Kolbe (Biomodels Austria and Institute for Biotechnology in Animal Production, IFA-Tulln, Austria)
  • Takashi Kuramoto (Institute of Laboratory Animals, Kyoto University, Kyoto, Japan)
  • Liangxue Lai (Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, PR China)
  • Jinsong Li (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Ning Li (State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing, PR China)
  • Kent Lloyd (University of California, Davis, CA, USA)
  • Shoukhrat Mitalipov (Oregon National Primate Research Center, OHSU, Beaverton, OR, USA)
  • Naomi Nakagata (Center for Animal Resources and Development, Kumamoto University, Japan)
  • Catheryn O’Brien (The Walter and Eliza Hall Institute, Melbourne, Australia)
  • Masaru Okabe (Genome Information Research Center Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan)
  • Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA)
  • Xin-an Pu (The Ohio State University, Comprehensive Cancer Center, Columbus, OH, USA)
  • Ling Sun (Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, PR China)
  • Davor Solter (Institute of Medical Biology, Singapore) Opening Talk
  • Zhu-Gang Wang (Shanghai Research Center for Model Organisms, Shanghai, PR China)
  • Guoliang Xu (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Bo Zhang (College of Life Sciences, Peking University, Beijing, PR China)
  • Qi Zhou (The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)

The list of topics that will be covered at the TT2013 meeting includes the following themes:

  • Androgenetic haploid embryonic stem cells
  • Sperm Cryopreservation and IVF
  • KOMP ES cell clones performance
  • Australian Phenomics Network Initiatives
  • Epigenetics and Transgenesis
  • Effects of in vitro culture on mammalian embryos
  • Epigenetic alterations during mouse transgenesis and cloning
  • Epigenetic reprogramming by oocytes
  • Targeted nucleases, ZFNs and TALENs, in transgenesis
  • Livestock Genome Editing with TALENS
  • Transgenic mice and ZFNs
  • KO pigs and ZFNs
  • Zebrafish and TALENs
  • Transgenic livestock
  • Ethics, animal welfare and regulations
  • Health monitoring protocols and transgenic facilities
  • Animal welfare and international mouse consortia
  • Impact of genetic background in mouse and rat models
  • Assisted reproduction strategies in mice
  • Non-rodent transgenesis
  • Primate chimeras and ES cells
  • Rat functional genomic initiatives
  • National BioResource Project for the rat
  • Animal models of human diseases
  • ES and iPS cells
  • transposon (PiggyBac)-mediated mutagenesis
  • Round-table discussion: Running a transgenic facility
  • Running a transgenic facility: The business aspect of running a transgenic unit
  • Running a transgenic facility: managing errors, difficulties
  • Running a transgenic facility: Staying on the cutting edge of technology while still maintaining basic services
  • Rat/mouse chimeras and iPS/ES cells
  • Overview of cre-transgenic mouse lines resources
  • China/Asia/Oceania in the International Mouse Functional Genomics Consortia
  • The Shanghai Research Center for Model Organisms

The TT2013 meeting will be held at the Baiyun International Convention Center-Oriental International Convention Hotel in Guangzhou, the largest comprehensive five-star hotel in Southern China, with an established record of hosting successful meetings and perfect supporting facilities. Located on the Baiyun Mountain, in the Baiyun New Town and overlooking the Baiyun Mountain Scenic Area and the Oriental Resort, this Convention Center is a fully equipped, self-contained meeting resort. More than 40 international air routes serve the new Baiyun International Airport, a 20 minute drive from the Baiyun International Convention Center.

After the TT2013 meeting, there will be a 3-days hands-on practical workshop on transgenic techniques, organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair; Ming Zhao (Southern Medical University, Guangzhou, China); Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China). This workshop is limited to 30 participants. Instructors for the practical course will include: Wenhao Xu (USA), Xin-an Pu (USA), Anna Auerbach (USA), Lily Reed (USA), Xin Rairdan (USA), Barbara Stone (USA), Steven Xing (USA), Margaret Landis (USA), Katja Becker (Germany), Ronald Naumann (Germany). Techniques that will be discussed and demonstrated at this course include: basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management,… This practical course will be co-sponsored by Hamilton-Thorne, ParaTechs, Sutter/Primetech. Registration for this 3-days practical workshop must be done through tt2013@transtechsociety.org and the TT2013 meeting registration web page. Workshop cost: 400$ (USD).

Registration fee to attend the TT2013 meeting begins at 400$(USD) and extends up to 630$(USD). ISTT members and technician/student participants are entitled to reduced registration fees.

Accommodation is NOT included in the registration fee and lodging must be reserved independently at the Guangzhou Baiyun International Convention Center, where a limited number of rooms have been prebooked for participants attending the TT2013 meeting at convenient reduced prices (610 RMB-single/680 RMB-double room). Note: RMB are China Yuan Renminbi, 100 RMB ~ 16 USD/12 Euros. Please check for most updated currency exchange.

The International Society for Transgenic Technologies (ISTT) will be sponsoring several Registration Awards for ISTT members willing to attend the TT2013 meeting. Applications for ISTT Registration Awards to attend the TT2013 meeting will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Registration Awards.

The International Society for Transgenic Technologies (ISTT), in collaboration with inGenious Targeting Laboratory (iTL), has establised the ISTT YOUNG INVESTIGATOR AWARDthat will be given at the Transgenic Technology (TT) Meetings. Nominations for the 2nd ISTT Young Investigator Award, for the TT2013 meeting, will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Young Investigator Awards.

Sponsoring companies willing to support the TT2013 meeting and to exhibit at the TT2013 meeting venue should contact TT2013 organizers through the TT2013 meeting email address (tt2013@transtechsociety.org). TT2013 meeting sponsorship opportunities are available at the meeting web site.

TT2013 meeting deadlines

Abstract submission deadline:  October 15, 2012
Application for ISTT registration awards:  October 15, 2012
Awards to be communicated by October 30, 2012
Early bird registration deadline: October 15, 2012
Standard fee registration deadline: January 31, 2013
Late registration fees & on-site registration: February 01, 2013

See you all in Guangzhou, at the TT2013 meeting!

Lluis Montoliu, PhD; President of the ISTT

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Posted in animal biotechnology, animal experimentation, co-sponsorhip, course, embryos, ES cells, general, information, iPS cells, ISTT, ISTT booth, ISTT Members, ISTT Prize, knock-outs, knockout, meetings, practical course, registration awards, rodent genetics, sperm, transgenic research, travel awards, TT meeting, web site, workshop, Young Investigator Award | No Comments »

The TT2013 meeting in China: progress and updated information

Friday, April 27th, 2012
The TT2013 meeting in China: progress and updated information

The TT2013 meeting in China: progress and updated information

The organization of the 11th Transgenic Technology meeting (the TT2013 meeting), to be held in Guangzhou, PR China, on February 25-27, 2013,  is progressing well. Prof. Ming Zhao (Southern Medical University in Guangzhou), Chair of the TT2013 Organization, and all his colleagues in the various advisory and supporting committees, are doing a great job and, hence, from the International Society for Transgenic Technologies (ISTT) we are sure that the TT2013 meeting is going to be yet another great transgenic conference for all of us to learn, discuss and enjoy.  The preliminary TT2013 meeting web page already informs about the confirmed topics and speakers that will be in Guangzhou. These will be regularly updated as we receive the confirmations from all invited speakers. Registration is expected to be open in June. The registration scheme and registration fees will be similar to the previous TT2011 meeting. The TT2013 meeting will be followed by a 3-day practical hands-on course on basic techniques in transgenesis, coordinated by Dr. Wenhao Xu (University of Virginia, Charlottesville, VA, USA).

Currently, the confirmed topics and speakers attending the TT2013 meeting include:

Meeting Topics

  •  Sperm Cryopreservation and IVF
  • KOMP ES cell clones performance
  • Epigenetics and Transgenesis
  • Effects of in vitro culture on mammalian embryos
  • Targeted nucleases, ZFNs and TALENs, in transgenesis
  • Transgenic pigs and TALENs
  • Transgenic mice and ZFNs
  • KO pigs and ZFNs
  • Zebrafish and TALENs
  • Ethics, animal welfare and regulations
  • Health monitoring protocols and transgenic facilities
  • Non-rodent transgenesis
  • Primate chimeras and ES cells
  • Rat functional genomic initiatives
  • Animal models of human diseases
  • ES and iPS cells
  • transposon (PiggyBac)-mediated mutagenesis
  • Round-table discussion: Running a transgenic facility
  • Running a transgenic facility: The business aspect of running a transgenic unit
  • Running a transgenic facility: managing errors, difficulties
  • Running a transgenic facility: Staying on the cutting edge of technology while still maintaining basic services
  • Rat/mouse chimeras and iPS/ES cells
  • Overview of cre-transgenic mouse lines resources
  • China/Asia/Oceania in the International Mouse Functional Genomics Consortia
  •  3-days hands-on practical workshop on transgenesis procedures after the TT2013 meeting
  • ..

Confirmed Speakers

  • Allan Bradley (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK)
  • Alan Colman (Institute of Medical Biology, Singapore)
  • Scott Fahrenkrug (Recombinetics, Minneapolis, MN, USA)
  • Malcolm France (Sydney University, Sydney, Australia)
  • Xiang Gao (Model Animal Research Center of Nanjing University, Nanjing, PR China)
  • Alfonso Gutiérrez-Adán (Dep. Animal Reproduction, INIA, Madrid, Spain)
  • Yann Herault (Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France)
  • Dietmar Kappes (Fox Chase Cancer Center, Philadelphia, PA, USA)
  • Liangxue Lai (Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, PR China)
  • Kent Lloyd (University of California, Davis, CA, USA)
  • Shoukhrat Mitalipov (Oregon National Primate Research Center, OHSU, Beaverton, OR, USA)
  • Naomi Nakagata (Center for Animal Resources and Development, Kumamoto University, Japan)
  • Catheryn O’Brien (The Walter and Eliza Hall Institute, Melbourne, Australia)
  • Masaru Okabe (Genome Information Research Center Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan)
  • Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA)
  • Ling Sun (Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, PR China)
  • Bo Zhang (College of Life Sciences, Peking University, Beijing, PR China)
  • Qi Zhou (The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)

From the ISTT, as in previous TT meetings, we will also promote and support the participation of ISTT members by sponsoring several registration awards for ISTT Members. Deadline for submitting applications for these ISTT Registration Awards is October 15, 2012. Instructions to apply can be found at the corresponding web page of the ISTT web site.

Please, write down the dates of the TT2013 meeting: February 25-27, 2013, and make sure you don’t miss this conference!. See you all in China next year!

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Posted in animal experimentation, animal welfare, bioethics, course, cryopreservation, embryos, ES cells, facility, genes, iPS cells, ISTT, ISTT Members, ISTT Prize, meetings, practical course, registration awards, transgenic, transgenic research, TT meeting, workshop | No Comments »

Life in the Fast Lane: an excellent overview

Wednesday, April 18th, 2012

Recently published in Cell, the paper titled “Life in the Fast Lane: Mammalian Disease Models in the Genomics Era” by Lukas Dow and Scott Lowe (vol 148, issue 6, 16 March 2012, p. 1099-1109) provides an excellent overview of the various methods and techniques that are available to develop rodent models of disease, as diagrammed in the picture here from the same paper.  It’s a worthwhile read for anyone in our field.

Posted in iPS cells, knock-outs, mouse strains, transgenic research | No Comments »

The transgenic-list (tg-l) has been moved to ISTT

Wednesday, December 21st, 2011
The transgenic-list (tg-l) has been moved to ISTT

The transgenic-list (tg-l) has been moved to ISTT

Today, December 21st, 2011, the historical transgenic-list (tg-l), created by Peter Sobieszczuk more than 15 years ago, in July, 1996, has been moved and successfully transferred to its new home at the ISTT web server, following the agreement signed on September 30th, 2011, between Peter and the ISTT. All current subscribers have been transferred automatically and they will be able to continue using and enjoying this useful resource within the field of transgenic animals. This is the new tg-l home web page, where additional information about this transfer and the new features of the tg-l at the ISTT can be browsed.  At the ISTT, the tg-l will continue being public (anyone may subscribe, including ISTT members and non-members alike, but subject to email address verification), unmoderated (messages will not be altered by the list administrator) and closed (only subscribers may post messages). All questions, comments, requests, doubts should be addressed to the new tg-l administrators at: tglist_admin@transtechsociety.org.

 

 

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Posted in embryos, ES cells, facility, gene trap, general, genes, information, iPS cells, ISTT, knock-outs, knockout, Manual, meetings, phenotyping, pigmentation, rodent genetics, rules, service, sperm, tg-list, transgenic, transgenic research, web site | No Comments »

Advanced Protocols for Animal Transgenesis. An ISTT Manual

Friday, September 9th, 2011
Advanced Protocols for Animal Transgenesis. An ISTT Manual

Advanced Protocols for Animal Transgenesis. An ISTT Manual

The International Society for Transgenic Technologies (ISTT) is most pleased to announce the publication of its first book, entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thom Saunders, and published by Springer (2011), in collaboration with the ISTT. This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists, most of them members of the ISTT, who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement. This book comprises 26 chapters,  109 illlustrations and 669 pages.

Table of contents, chapters of this ISTT Manual:

  • 1 Patent and Licensing Issues in Transgenic Technology. Karen S. Canady
  • 2 Global Resources: Including Gene Trapped ES Cell. Clones – Is Your Gene Already Knocked Out?. Lluis Montoliu
  • 3 Designing Transgenes for Optimal Expression. Eduardo Moltó, Cristina Vicente-García, and Lluis Montoliu
  • 4 Gene Targeting Vector Design for Embryonic Stem Cell Modifications. Thomas L. Saunders
  • 5 Transgenic Production Benchmarks. Thomas J. Fielder and Lluis Montoliu
  • 6 Generation of Transgenic Mice by Pronuclear Microinjection. Katja Becker and Boris Jerchow
  • 7 Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, and Ignacio Anegon
  • 8 Generation of Transgenic Animals by Use of YACs. Almudena Fernández, Diego Muñoz, and Lluis Montoliu
  • 9 BAC Transgenes, DNA Purification, and Transgenic Mouse Production. Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
  • 10 Generation of Transgenic Animals with Lentiviral Vectors. Carlos Lois
  • 11 Vertebrate Transgenesis by Transposition. Aron Geurts, Darius Balciunas, and Lajos Mates
  • 12 Rat Spermatogonial Stem Cell-Mediated Gene Transfer. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, and F. Kent Hamra
  • 13 Mouse Cloning by Nuclear Transfer. Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
  • 14 Gene Targeting in Embryonic Stem Cells. Elizabeth D. Hughes and Thomas L. Saunders
  • 15 The Importance of Mouse ES Cell Line Selection. Wojtek Auerbach and Anna B. Auerbach
  • 16 Tetraploid Complementation Assay. Marina Gertsenstein
  • 17 Combining ES Cells with Embryos. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, and Marina Gertsenstein
  • 18 Derivation of Murine ES Cell Lines. Kristina Nagy and Jennifer Nichols
  • 19 Rat Embryonic Stem Cell Derivation and Propagation. Ping Li, Eric N. Schulze, Chang Tong, and Qi-Long Ying
  • 20 Induced Pluripotency: Generation of iPS Cells from Mouse Embryonic Fibroblasts. Han Li, Katerina Strati, Verónica Dom?ínguez, Javier Martín, María Blasco, Manuel Serrano, and Sagrario Ortega
  • 21 The Preparation and Analysis of DNA for Use in Transgenic Technology. Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
  • 22 Colony Management. Karen Brennan
  • 23 Cryopreservation. Belén Pintado and Juan de Dios Hourcade
  • 24 Shipment of Mice and Embryos. Shirley Pease
  • 25 Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy. Jorge M. Sztein, R.J. Kastenmayer, and K.A. Perdue
  • 26 Refinement, Reduction, and Replacement. Jan Parker-Thornburg

ISTT Members are entitled to purchase this ISTT Manual with a 33% discount, through the members-only area of the web site.

Posted in animal biotechnology, animal experimentation, animal welfare, coat color, embryos, ES cells, facility, gene trap, general, information, iPS cells, ISTT, ISTT Members, Manual, publication, Springer, transgenic, transgenic research, web site | No Comments »

8th Transgenic Animal Research Conference: new strategies applied to livestock transgenesis

Thursday, August 11th, 2011
James D Murray, Organizer of the 8th UC Davis Transgenic Animal Conference

James D Murray, Organizer of the 8th UC Davis Transgenic Animal Conference

James D Murray (Department of Animal Science, UC Davis, and ISTT member) must be praised once again for having organized another successful edition (8th) of the highly interesting and stimulating Transgenic Animal Research Conference in Tahoe, a spectacular and most scenic high-mountain area in Northern California. Over 100 scientists, PhD students, biotechnologists and members of regulatory agencies have discussed, during three and a half days (August 7-11), the most recent advances in the generation, use and applications derived from geneticall modified animals, with a focus in non-rodent large animal models. The International Society for Transgenic Technologies (ISTT) is proud to have co-sponsored this conference, highly relevant for those working with transgenic pigs, sheep, cows, goats, chicken and fish, among other species. Several ISTT members have participated in this meeting, as invited speakers, session chairs or delegates. The abstracts of this meeting as well as a scientific report will be published in Transgenic Research, the scientific journal associated with the ISTT, freely available online to all ISTT Members.

ISTT supporting the 8th Transgenic Animal Research Conference

ISTT supporting the 8th Transgenic Animal Research Conference

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Posted in animal biotechnology, animal experimentation, embryos, ES cells, general, information, iPS cells, ISTT, ISTT booth, legislation, Meeting report, meetings, membership, transgenic research, web site | No Comments »

1st ISTT Young Investigator Award goes to Xiao-Yang Zhao

Thursday, August 4th, 2011
Prof. Xiao-Yang Zhao, 1st ISTT Young Investigator Award - 2011

Prof. Xiao-Yang Zhao, 1st ISTT Young Investigator Award - 2011

The International Society for Trangenic Technologies (ISTT) is pleased to announce the first ISTT Young Investigator Award, generously sponsored by inGenious Targeting Laboratory. From the four excellent candidates nominated by ISTT Members, the Award Committee chose Prof. Xiao-Yang Zhao, from the State Key Lab of Reproductive Biology (SKLRB), Institute of Zoology (IOZ), Chinese Academy of Sciences (CAS), Beijing, PR China, [nominated by Prof. Qi Zhou (ISTT Prize award 2004)]. The Award Committee felt his research was exciting and has great potential to impact the field of animal transgenesis, through his impressive work and publications on induced-pluripotent stem cells (iPS).

Dr. Xiao-Yang Zhao developed a system to generate induced pluripotent stem (iPS) cells  that showed 100 fold improvement in efficiency over the control method.  iPS cells show great promise in applications to develop regenerative medicine applications and generation of experimental animal models. Dr. Zhao’s breakthrough research proved that iPS cells could be used to generate viable mice by tetraploid complementation. This demonstration of complete iPS cell pluripotency was published in Nature in 2009 and received much attention from stem cell biologists and the popular media. Time Magazine named this research as the fifth most important medical breakthrough 2009 in a list of the top ten medical advances.

Dr. Xiao-Yang Zhao was born in 1980, in Southwest China. He received his PhD degree in Developmental Biology from Institute of Zoology, Chinese Academic of Sciences in 2010. After that, he was recommended and awarded to be the Young Principal of Investigator of IOZ, CAS. Dr. Xiao-Yang Zhao and colleagues found that some iPS cells failed to generate iPS mice by tetraploid complementation. However, when their nuclei were transferred to enucleated oocytes, cloned animals could be generated. This combination of using iPS cells from transgenic animals and nuclear transfer technologies will accelerate studies of transgenesis in non-rodent animals, specifically in agriculturally important species.

Award committee:

Dr. Thom Saunders, Univ. Michigan, Ann Arbor, MI, USA, Vice-President of ISTT, Chair
Prof. Francis Stewart, Biotechnologisches Zentrum, TU Dresden, Germany, ISTT Prize award 2010
Prof. Brigid Hogan, Duke University, Durham, NC, USA, ISTT Prize award 2008
Dr. Lisa Aronov, inGenious Targeting Laboratory, ISTT Young Investigator Award Sponsor

Dr. Xiao-Yang Zhao will attend the 10th Transgenic Technology (TT2011) meeting where he will deliver a short talk summarizing his scientific achievements that led him to receive this 1st ISTT Young Investigator Award, sponsored by inGenious Targeting Laboratory.

ISTT Young Investigator Award

ISTT Young Investigator Award

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Posted in general, iPS cells, publication, TT meeting, Young Investigator Award | No Comments »

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