Archive for the ‘ES cells’ Category

Mouse Genetics. Methods and Protocols (2014)

Saturday, August 9th, 2014
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Mouse Genetics. Methods and Protocols (2014)

Mouse Genetics. Methods and Protocols (2014)

This is yet another interesting book in our field that has been published this year, 2014. This manual, entitled “Mouse Genetics. Methods and Protocols“, edited by Shree Ram Singh and Vinzenzo Coppola, in association with the Publisher, Humana Press/Springer, contains a collection of useful protocols covering most of the methods that can be currently applied for the genetic modification of the mouse genome. According to its presentation at the Springer web page, this book “provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. (…) Each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.

The table of contents of this book illustrates the variety of highly sophysticated methods, beyond standard techniques, that are discussed here in detail, around mouse genetics, including: pronuclear injection-based targeted transgenesis through Cre-loxP specific recombination, the use of recombinase-mediated cassette exchange (RMCE) strategies, several approaches for preparing and analyzing conditional mutant alleles using tamoxifen-dependent Cre recombinases, the use of ICSI for the generation of transgenic mice, the use of BACs, mosaic analysis with double markers (MADM) in mice, transposon-mediated transgenesis, overexpression of microRNAs using Rosa26-mediated recombination, the isolation of various somatic and pluripotent cells, the generation of transgenic mice through spermatogonial stem cells in vivo, and, several illustrative examples of how different mouse engineered animal models are best suited to study a variety of human diseases. Hence, this book is also complementary to other recently published manuals, since it contains a careful detailed description of new methods that are not been covered in other similar titles in the field.

The editors of this book, Shree Ram Singh and Vinzenzo Coppola, have counted with the generous expertise shared and provided by a very large list of co-authors, including some ISTT members: Masato Ohtsuka, Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy, Kay-Uwe Wagner, Petra Kraus, V. Sivakamasundari, Xing Xing, Thomas Lufkin, Anton J.M. Roebroek, Bart Van Gool, Kun-Hsiung Lee, Susanne Feil, Jana Krauss, Martin Thunemann, Robert Feil, Pedro N. Moreira, Lluis Montoliu, Jane Beil, Thorsten Buch, Sheng Ding, Tian Xu, Xiaohui Wu, Hui Zong, Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, Olga Simmons, Esther M. Bolanis, Jian Wang, Simon J. Conway, Kanika Jain, Paul J. Verma, Jun Liu, Pollyanna Agnes Goh, Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, Mugdha V. Joglekar, Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, Margaret H. Baron, Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, Yang Du, Lalit Sehgal, Abul Usmani, Sorab N. Dalal, Subeer S. Majumdar, Spencer W. Luebben, Naoko Shima, Tsuyoshi Kawabata, Robert M. Hoffman, Viive M. Howell, Emily K. Colvin, Vishalakshi Chavali, Shyam Sundar Nandi, Paras Kumar Mishra, Julia Lorenz, Susanne Grässel, Ganesan Ramesh, Punithavathi Ranganathan, Santhakumar Manicassamy, Indumathi Manoharan, Deepak P. Patil, Holly D. Kristensen and Yogesh Shouche.

This new book will be added to the collection of Springer books published on animal transgenesis and animal genetics for which ISTT members are entitled to a 33% discount, as one of the many benefits associated with the ISTT membership.

 

 

Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Sunday, July 27th, 2014
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Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Twenty years after the publication of the first edition and twelve years after the release of the second edition of this book, Carl A. Pinkert (Auburn University, College of Veterinary Medicine, Auburn, AL, USA) in association with Elsevier, releases now the third edition of his famous transgenic manual: “Transgenic Animal Technology. A Laboratory Handbook. 3rd edition, 2014“. As it will be familiar to readers of the two previous editions of this useful and unique handbook, this is not only a manual to understand how to make a transgenic mouse. This handbook looks beyond mice and it contains protocols to prepare a wide variety of genetically-modified animals, including: rats, rabbits, poultry, fish, pigs, ruminants and non-human primates. In addition, this compilation of helpful methods includes a number of chapters devoted to understand and improve all steps of transgenesis, from vector design, analysis of transgene integration and the evaluation of transgene expression. Finally, the book also includes cryopreservation methods, an embryo culture section, a review of standard nomenclature and a selection of databases and internet resources currently available in the field.

This handbook is a worth addition to any library, laboratory or transgenic facility, complementary to other available manuals on the subject, but unique in the sense that it exquisitely illustrates current transgenic methods that can be applied to a wide variety of animal species, beyond mice.

Carl A. Pinkert has been helped in his outstanding Editorial task by a large group of co-authors, experts in their subjects, including some ISTT members: Satoshi Akagi, Anna V. Anagnostopoulos, Benjamin P. Beaton, Cory F. Brayton, Steve Brown, Anthony W.S. Chan, Tom Doetschman, Rex A. Dunham, David A. Dunn, Janan T. Eppig, Almudena Fernandez, Tatiana Flisikowska, Vasiliy Galat, Robert A. Godke, Philip Iannaccone, Michael H. Irwin, Larry W. Johnson, Yoko Kato, Teoan Kim, Alexander Kind, Bon Chul Koo, Mo Sun Kwon, Daniel J. Ledbetter, Michael J. Martin, Kazutsugu Matsukawa, Colin McKerlie, Lluis Montoliu, Paul E. Mozdziak, Akira Onishi, Paul A. Overbeek, James N. Petitte, L. Philip Sanford, Jorge A. Piedrahita, Wendy K. Pogozelski, H. Greg Polites, Edmund B. Rucker III, Marina Sansinena, Angelika Schnieke, Kumiko Takeda, James A. Thomson, Ian A. Trounce, Yukio Tsunoda, Cristina Vicente-Garcia, Kevin D. Wells, Richard N. Winn and Curtis R. Youngs.

Manipulating the Mouse Embryo. A Laboratory Manual (4th edition, 2014)

Tuesday, July 22nd, 2014
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Manipulating the Mouse Embryo. A Laboratory Manual (4th edition, 2014)

Manipulating the Mouse Embryo. A Laboratory Manual (4th edition, 2014)

A bit more than 10 years later, from the last edition (3rd, 2003) published, the classical manual, the “Bible” in our field has been renewed, updated, upgraded and recently published in its fourth edition. The new “Manipulating the Mouse Embryo. A Laboratory Manual. 4th edition, 2014” has just been released, published by Cold Spring Harbor Laboratory Press and edited by Richard Behringer, Marina Gertsenstein, Kristina Vintersten Nagy and Andras Nagy. The editors, the same team that produced the 3rd edition of this useful manual, should be praised once again for a brilliant work done, accommodating the latest techniques in mouse embryo manipulation, while not forgetting the traditional procedures that must be learnt properly by anyone joining this field of animal transgenesis for the first time.  The editors counted with the help of many additional collaborators, who provided materials and protocols, including many ISTT members, as Marina and Andras are, such as:  Wojtek Auerbach, Ralph Brinster, Jorge Cabezas, Tracy Caroll, Lluis Montoliu, Naomi Nakagata, Jan Parker-Thornburg, Shirley Pease and Thomas Saunders, just to name a few of the many colleagues that shared their expertise and contributed to this 4th edition of the CSHLP manual.

This fourth edition is dedicated to Anne McLaren (1927-2007), one of the brave and gifted pioneer in mouse genetics and embriology, and whom do we all owe a number of standard procedures that we routinely apply in a transgenic laboratory, such as mouse embryo culturing, pseudopregnancy and embryo transfer.

The structure of this 4th edition manual follows that of the 3rd edition, in the sense that chapters and methods are grouped logically and functionally, from a very good summary on mouse genetics and mouse embryo development, to mouse colony management, and followed by a variety of main sections dealing with in vitro and in vivo work, surgical procedures, the production of transgenic mice by pronuclear microinjection, derivation of ES cell lines, using ES cells to generate germline-transmitting chimeras, genotyping protocols, parthenogenesis and nuclear transfer, assisted reproduction techniques, cryopreservation methods, techniques for visualizing gene products and finishing by a chapter devoted to setting up a micromanipulation laboratory and the usual and convenient appendix with receipts for the common buffers and solutions.

Besides this excellent updated version of the CSHLP Mouse Manual, there are several books published on this subject, including the ISTT manual (Springer, 2011) edited by Shirley Pease and Thomas Saunders, and all are worth reading and having them around for consultation, since each one brings their own contribution, their view and their solutions for the adequate learning of how to best manipulate animal embryos, and not all methods and protocols are covered in a single book, hence they are largely complementary. I will be soon reporting in this ISTT blog about additional books recently published in this field.

 

TT2014 abstracts: submission deadline is approaching (30 June)

Tuesday, June 17th, 2014
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TT2014 abstracts: submission deadline is approaching (30 June)

TT2014 abstracts: submission deadline is approaching (30 June)

From the International Society for Transgenic Technologies (ISTT) we warmly invite and encourage you all to submit your most recent and exciting results and developments in animal transgenesis to be presented at the forthcoming 12th Transgenic Technology (TT2014) meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014. Deadline for submitting abstracts for the TT2014 meeting is June 30.
To submit an abstract please visit this TT2014 meeting web page.

All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements:

  • Title (max. 25 words)
  • Name authors and affiliations (first author is the presenting author).
  • Text of the communication (max. 400 words).
  • Abstracts should be submitted no later than June 30, 2014.

Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards.

Best Poster Awards
All posters presented at the TT2014 meeting will be eligible for one of the ISTT Best Poster Awards, generously sponsored by Charles River, Inc.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

We are looking forward to receiving your exciting works to discuss the latest development on animal transgenesis!. See you all in Edinburgh!

Highlights of the TT2014 meeting in Edinburgh: a conference you can’t miss!

Thursday, June 5th, 2014
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The TT2014 meeting in Edinburgh (6-8 October 2014)

The TT2014 meeting in Edinburgh (6-8 October 2014)

This year’s ISTT main activity is the 12th Transgenic Technology conference, the TT2014 meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014, followed by a 2-day hands-on workshop on basic zebrafish transgenesis techniques. The ISTT promotes the TT meetings every 18 months, these being the most important activity of our Society. This year, the local Organizers and advisory committees, commanded by Douglas Strathdee, need to be praised for preparing a most appealing and interesting program, addressing hot topics, current and most up-to-date issues actively discussed nowadays by the transgenic animal community. Talks that will be presented by the most active and prestigious scientists in our field.

Why you shouldn’t miss the TT2014 meeting?

  • If you are interested in the new transgenic methods associated to nucleases (ZFNs, TALENs and CRISPRs-Cas9) there will be plenty of  interesting talks where these new fantastic tools will be presented and discussed, directly by the key players in this rapidly-evolving field, including: Rudolf Jaenisch, William Skarnes, Angelika Schnieke, Kai Schönig, Ignacion Anegon, Pawel Pelczar, Francis Stewart, Keith Joung and Feng Zhang. And, most likely, these techniques will be referred and cited in many additional talks too, including the round table discussion about the future of transgenic core facilities, chaired by James Bussell.
  • If you are interested in ES cell biology and in innovative uses of ES cells and associated technologies there will be unique talks delivered by Jos Jonkers, Austin Smith, Ian Chambers, Janet Rossant and Alex Joyner
  • If you are interested in phenotyping your mouse animal models there will be fantastic talks delivered by  Jacqueline White, Stephen Murray, David Adams, Daniel MurphyAnna-Katerina Hadjatonakis and Vasilis Ntziachristos
  • If you are interested in non-rodent, large mammals and birds, animal models there will be great talks by James Murray, Angelika Schnieke, Mike McGrew and Adrian Shermann
  • If you are interested in rats there will be compelling talks by Kai Schönig and Ignacio Anegon
  • If you are interested in zebrafish animal models there will be fascinating talks by Stephen Ekker, Koichi KawakamiKeith Joung and Elizabeth Patton
  • If you are interested in animal welfare and 3Rs, in the best use of our laboratory animals, there will be captivating talks by Peter Hohenstein, Sara Wells and Jan-Bas Prins.

Therefore, there will be really engaging talks interesting to everyone in our field. This is why you shouldn’t miss this great and unique opportunity!.

Register now for the TT2014 meeting. Submission of abstracts will be accepted up to June 30. Early-Bird registration at reduced fees will be promoted up to July 31. ISTT members are entitled to reduced fee registration.

See you all in Edinburgh in October!

 

Meeting report: Promoting the international exchange of mouse mutant resources

Monday, May 12th, 2014
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Infrafrontier-IMPC workshop: Promoting the international exchange of mouse mutant resources, Munich, Germany, 8-9 May 2014

Infrafrontier-IMPC workshop: Promoting the international exchange of mouse mutant resources, Munich, Germany, 8-9 May 2014

This is a brief meeting report on the INFRAFRONTIER /IMPC workshop: Promoting the international exchange of mouse mutant resources, which was held in Munich, Germany, on 08-09 May 2014.
As indicated in the corresponding Infrafrontier web page: “The main objectives of the workshop were to discuss how to simplify the international exchange of mouse mutant resources and to define the procedural changes to achieve it, to review the key issues facing the mouse community and mouse repositories as well as focus on IP issues and to present best practices in sharing research tools. The workshop was targeted at the directors of major mouse repositories, IP and technology transfer experts, representatives of scientific journals and funders and attracted the attention of 70 participants.” Delegates from major mouse repositories (JAX, MMRRC, EMMA, CMMR, RIKEN BRC, CARD, MARC), mouse international projects and consortia (EUCOMM, EUCOMMTOOLS, KOMP, KOMP2, IKMC, IMPC, KMPC), other related consortia (SGC), scientific journals (Nature, PLOS), funding agencies (NIH), companies (BioDoc, Charles River, AddGene), associations (AMMRA, AMPC, FELASA, EARA), TTOs and lawyers from numerous institutions and end-users gathered to discuss about how to best promote the international exchange of mouse mutant resources.

This workshop was funded by the EC FP7 InfraCoMP project. InfraCoMP’s main objective is to coordinate the collaborative efforts between the Infrafrontier Research Infrastructure and the International Mouse Phenotyping Consortium (IMPC). The scope of this Infrafrontier-IMPC workshop in Munich included various major topics, such as:

  • to discuss about simplified procedures to effectively exchange mouse mutant resources among repositories and between repositories and end-users/customers, trying to review and fix all restrictions preventing from adequately sharing major mouse mutant resources.
  • to review the key issues currently faced by the mouse community and mouse repositories, including emerging new genome editing technologies (ZFNs, TALENs, CRISPRs) and the role of mouse archives in the international exchange of mouse mutant resources
  • to discuss on IP issues and the administrative paperwork usually associated with any transactional international negotiation involving licenses and MTAs
  • to showcase best practices, examples of successful sharing research tools that could be applied on sharing mouse mutant resources

This workshop represented a continuation towards the eventual application of the agreements included in the so-called Rome Agenda, published in 2009 (Schofield et al. 2009, Nature) where the major headlines, best practices and recommendations concerning the deposit and sharing of biological resources, including mice, ES cells and germplasm, under the least restrictive terms possible, had been already discussed and identified but, unfortunately, not sufficiently widespread nor systematically followed, in spite of new initiatives adopted by some funding agencies, enforcing public-access policies for materials associated with projects funded by the NIH or the Wellcome Trust in order to receive the allocated funds.

The impact of the new genome editing technologies on current mouse consortia and mouse archives was discussed at length and in depth, from various angles and by different speakers. It is obvious that a new logic has emerged, the updated mouse genetics toolbox and its widespread among scientists enables them to generate their mouse mutants of interest through alternative, often faster approaches. Instead of considering the new endonuclease-mediated mutations solely a threat for traditional approaches, based on ES cell clones (however using higher genetic and quality-controlled standards), it was finally interpreted as an opportunity for mouse consortia and repositories. For example, the easier and faster generation of new mouse mutations could help finishing the functional annotations of the mouse genome, for all these loci that could not be targeted or, if targeted, did not result in the corresponding mouse strain through IKMC-IMPC current approaches.

The description of innovative shipment methods, for refrigerated biological materials, or using dry-ice, as compared to the standard but more complex liquid-nitrogen dry shippers was also discussed in order to make the distribution of mouse mutant resources cheaper and easier. The new set of sperm and oocyte cryopreservation methods and the optimized associated IVF procedures, as reported by CARD, Kumamoto University, in Japan, have also greatly contributed to promote the international exchange of mouse mutant resources, avoiding the always difficult and expensive shipment of live research laboratory animals.

The legal agreements, such as Material Transfer Agreements (MTAs), governing the access to mouse mutant resources were also discussed extensively. The complexity of some of these MTAs and the often long administrative process involved for executing them, unnecessarily extends the time required to access to a given mouse mutant strain deposited in a major repository for academic use. Interesting analyses of common practices observed within the international mouse community and applied by mouse consortia were presented (Bubela et al. 2012Mishra and Bubela, 2014). The overall recommendation was, whenever possible, avoid using specific MTAs and favor the unrestrictive distribution of mouse resources through simpler “conditions of use”, as regularly applied by The Jackson Laboratory (JAX) to all their mouse strains, and by EMMA-INFRAFRONTIER, for mouse lines non-associated to specific MTAs, in order also to reduce the administrative time to the minimum. In case MTAs should be included, for academic non-commercial use, the recommendations discussed were to simplify, and unify, the document as much as possible, ideally without requesting to disclose the field of use, without imposing reach through on modifications of the received materials and clearly defining third-party use after permission has been obtained. Attribution should also be clearly encouraged. Examples of simplified MTAs, also including useful institutional versions of these agreements, can be found at KOMP. The model deployed by AddGene, a non-profit organization dedicated to efficiently distribute plasmids among the scientific community, using also simple MTA procedures, was also presented as an example of successful solution.

Overall, this intense 2-day Infrafrontier-IMPC workshop fulfilled its aims and expectations. All stakeholders in the field could openly express their opinions, fears, opportunities, problems and solutions. The Organizers should be praised for their selection of speakers, topics and participants. Now it will be the time for the most difficult part: converting the agreements and recommendations into realities, while ensuring that researchers in academia, using mouse mutant resources, have an easier, simpler and faster access to mice and/or their associated products, for the benefit of science, and knowledge advance.

Infrafrontier-IMPC workshop: Promoting the international exchange of mouse mutant resources, Munich, Germany, 8-9 May 2014

Infrafrontier-IMPC workshop: Promoting the international exchange of mouse mutant resources, Munich, Germany, 8-9 May 2014

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

Sunday, May 11th, 2014
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Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

The submission of abstracts for the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh (Scotland, UK), on 6-8 October, is OPEN. All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements: Title (max. 25 words), Name authors and affiliations (first author is the presenting author), and, Text of the communication (max. 400 words). Abstracts should be submitted no later than June 30, 2014. Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards. All presented Posters at the TT2014 meeting will be entitled to the Best Poster Awards, generously sponsored by Charles River.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

Advertising the TT2014 meeting from your institutions: put one of these Posters!

Friday, February 28th, 2014
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12th Transgenic Technology (TT2014) meeting, Edinburgh, Scotland, UK, 6-8 October 2014

12th Transgenic Technology (TT2014) meeting, Edinburgh, Scotland, UK, 6-8 October 2014

The next ISTT meeting will be held in Europe this year. The 12th Transgenic Technology (TT2014) meeting, will take place in Edinburgh, Scotland, UK, on 6-8 October 2014, organized by ISTT members Douglas Strathdee (chair), Peter Hohenstein and Bruce Whitelaw, and hosted by three Scottish research institutes and the University of Edinburgh: the Roslin Institute; the Institute of Genetics and Molecular Medicine and the Beatson Institute for Cancer Research. The TT2014 meeting will be followed by the 2-day hands-on workshop “An Introduction to Zebrafish Transgenesis“, on 8-10 October 2014.

An outstanding group of invited speakers have confirmed their participation at the TT2014 meeting. Abstract submissions and application for the ISTT registration awards (for ISTT members) deadlines merge on 30 June 2014. Early bird registration deadline at reduced fees is 31 July 2014. A number of submitted abstracts will be selected for oral presentation on topics including:

  • new technologies in transgenesis
  • pluripotential stem cells
  • targeted nucleases and genome editing
  • models of human disease
  • animal ethics and welfare
  • large-scale phenotyping initiatives
  • animal biotechnology
  • in vivo imaging
  • zebrafish models and transgenesis

Douglas Strathdee and his colleagues have prepared the following collection of eight Posters to advertise the TT2014 meeting, illustrated with beautiful Edinburgh pictures. Please, help us announcing and disseminating the TT2014 meeting by putting one or several of these Posters at your centres, institutions, facilities, departments, universities. The TT meeting is a unique forum occurring every 18 months where to discuss the latest technical developments and applications on animal transgenesis. This is a conference that can’t be missed by anyone interested in this subject! Thanks for helping us advertise the TT2014 meeting!

TT2014 Poster version 1

TT2014 Poster version 1

TT2014 Poster version 1 (A4 format)
TT2014 Poster version 1 (A3 format)

TT2014 Poster version 2

TT2014 Poster version 2

TT2014 Poster version 2 (A4 format)
TT2014 Poster version 2 (A3 format)

TT2014 Poster version 3

TT2014 Poster version 3

TT2014 Poster version 3 (A4 format)
TT2014 Poster version 3 (A3 format)

TT2014 Poster version 4

TT2014 Poster version 4

TT2014 Poster version 4 (A4 format)
TT2014 Poster version 4 (A3 format)

TT2014 Poster version 5

TT2014 Poster version 5

TT2014 Poster version 5 (A4 format)
TT2014 Poster version 5 (A3 format)

TT2014 Poster version 6

TT2014 Poster version 6

TT2014 Poster version 6 (A4 format)
TT2014 Poster version 6 (A3 format)

TT2014 Poster version 7

TT2014 Poster version 7

TT2014 Poster version 7 (A4 format)
TT2014 Poster version 7 (A3 format)

TT2014 Poster version 8

TT2014 Poster version 8

TT2014 Poster version 8 (A4 format)
TT2014 Poster version 8 (A3 format)

A report on the 7th European Short Course on Laboratory Animal Science in Strasbourg, organized by Charles River

Friday, February 14th, 2014
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A report on the 7th European Short Course on Laboratory Animal Science in Strasbourg, organized by Charles River

A report on the 7th European Short Course on Laboratory Animal Science in Strasbourg, organized by Charles River

The 7th European Short Course on Laboratory Animal Science, organized by Charles River, just closed in Strasbourg, France, after three days of interesting presentations and discussions at the intersection between animal welfare, animal experimentation, current guidelines and legislation, biomedical research from academia and industry and society perception on these topics. The Organizers should be praised for the selection and variety of topics, as well as for the smooth and pleasant running of the entire course, which included an enjoyable visit to an old typical cellar from the Alsace region along with a wine-testing Gala dinner.

Several ISTT members participated in this event, including organizers (Cyril Desvignes, Jean Cozzi), members of the steering committee (Johannes Wilbertz), invited speakers (Belén Pintado, Yann Herault, Ignacio Anegon, Lluís Montoliu), and participants (Marcello Raspa, Ferenc Erdelyi, Gabor Szabo,…) among other.

During this course, the recent EU Directive 2010/63 on the protection of animals used in research and its implication on the use of animals in biomedical research and policies throughout Europe was discussed, from different angles, by Magda Chlebus, Gill Fleetwood, Thierry Decelle, Patri Vergara and Belén Pintado. Topics covered included the new training courses and competencies to work with experimentation animals in Europe, the animal-welfare bodies and the current understanding of the 3R’s paradigm. Javier Guillén compared, side by side, the new EU Directive with the current Guide in the US and highlighted their many coincidences, suggesting that a combined use of both documents would be ideal for the adoption of successful animal care and use programs. Jan-Bas Prins, current president of FELASA, presented his view of the field of laboratory animal sciences, before the implementation of this new EU Directive, as an opportunity and a positive challenge to interface and exchange knowledge with many other players involved.

Health monitoring programs, rodent microbiologic surveillance, methods employed to detect all these pathogens robustly in laboratory animal facilities and the updated recommendations from FELASA, recently published in Laboratory Animals, were presented by William Shek, Guy Mulder, Stéphanie Durand and Axel Kornerup Hansen. Operational and technical aspects of animal facilities were discussed by Alberto Gobbi and Peter Dockx, whereas the issues related with occupational health and safety program evaluations were presented by Jann Hau.

Examples of the use of rodent animal models in biomedical research, in academia, by James Di Santo and Andrea Bertotti, as well as in the industry, by Joyce L. Young, were discussed. The importance of genetic quality in mouse research as well as the complexity of mouse genome and the impact of the genetic background on phenotypes was presented by Charles Miller and Lluís Montoliu, respectively. The procedures conducted at the International Mouse Phenotyping Consortium (IMPC) as well as the challenges they encountered during the deployment of this impressively large enterprise were presented and discussed by Sara Wells and, by the local representative, Yann Herault, Director of the French Mouse Clinic, ICS, in Strasbourg, who delivered the closing talk.

The newest technologies in stem cell biology and animal transgenesis were also present at this 7th Short Course. Hongkui Deng summarized the most innovative approach he devised to prepare induced-pluripotent cells from somatic cells, using a cocktail of four chemicals, four molecules that mimicked the induction signals described by Shinya Yamanaka. The new logics for the production of targeted genetic modifications, using editing or engineered nucleases (Meganuclease, ZFNs, TALENs, CRISPRs) in mice and rats was presented by Ralf Kuehn and Ignacio Anegon, respectively.

The choice of rodent anaestesia protocols was discussed by Aurelie Thomas, whereas the various methods for euthanasia in rodents were presented by Huw Golledge. On the last day, Aurora Bronstad summarized the work done at the AALAS-FELASA joint working group on harm-benefit analysis, whereas Katrina Gore highlighted the need for more robust analytical procedures in research protocols involving animal experimentation, in order to optimize the rate of success of pre-clinical drugs.

In summary, the 7th Edition of this biennial Charles River Short Course on Laboratory Animal Science in Europe, attended by some 120 participants, was an excellent opportunity to update information related to animal welfare, EU legislation and transposition difficulties in various countries, newest technologies, mouse genomics and genetics, large mouse consortia and numerous important topics that are relevant for animal facility managers, researchers, veterinarians and anyone else interested in the best use of animals in experiments, according to current laws and recommendations.

More than 27,000 messages on animal transgenesis available to ISTT members through ISTT_list and tg-l archives

Sunday, February 9th, 2014
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More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

One of the most important assets of the International Society for Transgenic Technologies (ISTT), is the amount of information on animal transgenesis accummulated through the archives of the ISTT_list and tg-l email lists. Currently, more than 27,000 messages are fully available to ISTT members, conveniently organized in searchable and dynamic archives. The traditional transgenic-list (tg-l), operative since 1996 and offered from the ISTT web server since the end of 2011, has distributed over 22,000 messages since then, whereas the ISTT_list, associated and born with our Society in 2006, has disseminated some 5,000 messages, discussing both lists on almost each and every topic, issue or situation related directly or indirectly with animal transgenesis. All this endless information resource is fully available to ISTT members, through powerful search engines. Non-ISTT members subscribing to tg-l have access only to the most recent messages distributed through the tg-l, using the simple search engine, which allows simple searches and outputs the 50 most recent messages discussed on the subject of interest. In contrast, ISTT members have access to more sophysticated searching engines and the output always contains all messages archived on the matter investigated.

Obtaining granted access to these rich sources of information is very easy and cheap. Simply apply for ISTT membership! Submit now your application to become a member of the ISTT and you will get immediate and full access to all these messages.


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