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7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

Tuesday, February 12th, 2013
7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

7th Workshop on Innovative Mouse Models (IMM2013), Leiden, The Netherlands, 13-14 June 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the 7th Workshop on Innovative Mouse Models (IMM2013), which will be held at the Leiden University Medical Center (LUMC), in Leiden, The Netherlands, on 13-14 June 2013.  This will be the third consecutive edition of this meeting series that is granted with the co-sponsorship of the ISTT, after the IMM2011 and the IMM2009 editions, due to its interest and relevance for the community of researchers using genetically-modified mice. The IMM2013 workshop is organized by: Jos Jonkers (NKI-AVL, Amsterdam, The Netherlands), Paul Krimpenfort (NKI-AVL, Amsterdam, The Netherlands), Werner Mueller (University of Manchester, Manchester, United Kingdom), Hein te Riele (NKI-AVL, Amsterdam, The Netherlands), Els Robanus-Maandag (LUMC, Leiden, The Netherlands), Marian van Roon (VU, Amsterdam, The Netherlands and ISTT Member) and Sjef Verbeek (LUMC, Leiden, The Netherlands and ISTT Member).

According to the IMM2013 workshop web page: The primary goal of this two-day workshop is to bring together a diverse group of scientists interested in advanced genome alteration approaches in the mouse, including key developers of emerging technologies as well as researchers who wish to apply and assess these new approaches. The IMM2013 workshop will encourage an in-depth and unvarnished discussion of these technologies and novel developments. This 2-day workshop will have a mixture of invited speakers and selected presentations. Keynote lectures will be given by:

  • Anton Wutz (UK) haploid ESC
  • Haoyi Wang (US) iPS / TALENs
  • Bill Skarnes (UK) high throughput TALENs
  • Ben Davis (UK) docking site/ new recombinases
  • Yann Herault (France) Cre transgenic mice
  • Kevin Brindle (UK) MRI
  • Mathijs Verhagen (NL) large scale phenotyping
  • B Kappes (US) TALENs
  • Zoltan Ivics (Germany) Transposons
Registration will open soon. ISTT members will be entitled to a reduced registration fee.

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Posted in co-sponsorhip, ES cells, iPS cells, ISTT, ISTT Members, knockout, mouse strains, TALENs, targeted nucleases, transgenic research, web site, ZFNs | No Comments »

John B. Gurdon and Shinya Yamanaka awarded the 2012 Nobel Prize in Physiology or Medicine

Monday, October 8th, 2012
John B. Gurdon (left) and Shinya Yamanaka (right) awarded the 2012 Nobel Prize in Physiology or Medicine

John B. Gurdon (left) and Shinya Yamanaka (right) awarded the 2012 Nobel Prize in Physiology or Medicine

The Nobel Prize in Physiology or Medicine 2012 was awarded today, October 8, 2012, jointly to Sir John B. Gurdon (Gurdon Institute, Cambridge, United Kingdom) and Shinya Yamanaka ( Kyoto University, Kyoto, Japan, and Gladstone Institute, San Francisco, CA, USA) “for the discovery that mature cells can be reprogrammed to become pluripotent“. The Nobel Assembly at Karolinska Institutet (Stockholm, Sweden) decided to award The Nobel Prize in Physiology or Medicine 2012 jointly to John B. Gurdon and Shinya Yamanaka. According to the published Press Release: “The Nobel Prize recognizes two scientists who discovered that mature, specialised cells can be reprogrammed to become immature cells capable of developing into all tissues of the body. Their findings have revolutionised our understanding of how cells and organisms develop“. The International Society for Transgenic Technologies (ISTT) wishes to congratulate both excellent scientists for their outstanding achievements and the very much deserved Nobel Prize they have just been awarded.

John B. Gurdon demonstrated in 1962 that adult frogs could be obtained by the transplantation of nuclei from endoderm cells of Xenopus laevis donors ranging from late blastulae to swimming tadpoles.  In 1966, John B. Gurdon continued his studies on cellular reprogramming in frogs and reported that fertile adult male and female frogs, genetically marked as of solely donor origin, had been obtained from the transplantation of nuclei from intestinal epithelial cells of Xenopus laevis feeding larvae. In 1975, John B. Gurdon reported that tadpoles could be obtained from nuclei transplanted from keratinized skin cells of adult frogs. However, no adult frogs were obtained from nuclear transfer experiments involving adult somatic cells. As concluded by Gurdon and Byrne in 2003, these first series of results obtained by John B. Gurdon, “established the general principle that the process of cell differentiation does not necessarily require any stable change to the genetic constitution of a cell. Thus, cell differentiation depends on changes in the expression not content of the genome“. Similar principles had been envisaged and proposed, but could not be proved, in 1938 by the German embryologist Hans Spemann (awarded the 1935 Nobel Prize in Physiology or Medicine).

Forty years later, after the outstanding results obtained by John B. Gurdon, Shinya Yamanaka reported in 2006 the identification of  four transcription factors (Oct3/4, Sox2, c-Myc, and Klf4) capable of reprogramming any somatic cell into a cell with properties of a pluripotent stem cell, similar to embryonic stem (ES) cells. Those cells were named as inducible pluripotent stem cell (or iPS cells) and greatly revolutionized the regenerative medicine field and cellular reprogramming studies since then. Yamanaka’s experiment provided a totally innovative method to obtain human pluripotent stem cells, with great regenerative potential, without requiring the use of human embryos, an achievement that has been greatly acknowledged by members of the society who did not accept, according to their private beliefs, the previous use of human embryos to obtain pluripotent stem cells. John B. Gurdon and Shinya Yamanaka had already been awarded jointly the 2009 Albert Lasker Basic Medical Research Prize “for discoveries concerning nuclear reprogramming, the process that instructs specialized adult cells to form early stem cells — creating the potential to become any type of mature cell for experimental or therapeutic purposes“.

The history of nuclear transplantation and cellular reprogramming has been silently advancing since the beginning of last century, and progressed through a series of phenomenal milestones that were regularly achieved. Starting with the pioneer experiments and the vision of Hans Spemann (1938), then the first nuclear transfer success in frogs by Briggs and King (1952), of course the experiments carried out by John B. Gurdon in the 60′s and early 70′s, already mentioned, and also the studies by Marie DiBerardino (1967) on the effect of cell cycle in the nuclear transfer success, and other, more recent, describing the role of single transcription factor being able to change the fate of a cell (Harold Weintraub, 1987) or the direct reprogramming experiments, betweeen different cellular haematopoietic types, pioneered by Thomas Graf since 1990. However, in my opinion, the one single study that, for the first time, demonstrated that a nucleus from an adult terminally and fully differentiated somatic cell (i.e. a cell derived from mammary gland tissue) could give rise to a fertile normal adult, closing the circle of life, was the birth of Dolly, the sheep, obtained by Ian Wilmut and his collaborators from the Roslin Institute in 1996 and reported in a famous paper in Nature in February 1997.

Ian Wilmut and Dolly the sheep in 1997

Ian Wilmut and Dolly the sheep in 1997

The impact of Wilmut’s study on Biology, Biomedicine, Biotechnology and on the entire Society was phenomenal, tremendous (unfortunately not always positive, independent of him, with intense and never ending debates beyond science) and triggered many subsequent studies, including the use of human pluripotent stem cells, isolated by Thomson and Gearhart teams in 1998, and their potential in regenerative medicine, and eventually the work by Yamanaka and many other. The Nobel Assembly has limited this Nobel award to Gurdon and Yamanaka, who fully deserved the Nobel Prize, but, unfortunately, they did not include other scientists that were instrumental for the progress in reprogramming studies, particularly Ian Wilmut. Dolly’s experiment  is only referred briefly in the advance information provided to interested readers. After discussing this issue the whole day with many colleagues, I believe I speak on behalf of many by stating that a Nobel Prize awarded to Gurdon, Wilmut and Yamanaka, could have been a much better and balanced choice. Gurdon himself stated that he would have liked to share this Prize with Ian Wilmut.  But this is just wishful thinking and the reality is, as usual, different. Having said that, I want to congratulate once again John B. Gurdon and Shinya Yamanaka for this Nobel Prize.

On a final sad note, after all the excitement associated with this Nobel Award to reprogramming techniques, we have been informed of the sudden death of Keith H. Campbell, biologist (1954 – 2012), who passed away last Saturday, October 6, 2012. Keith H. Campbell was one of the main co-authors, together with Ian Wilmut, of Dolly’s Nature paper in 1997 (Viable offspring derived from fetal and adult mammalian cells. Wilmut I, Schnieke AE, McWhir J, Kind AJ, Campbell KH, Nature 1997 Feb 27;385(6619):810-3). Keith H. Campbell was currently working at the School of Biosciences, University of Nottingham, UK.

 

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Posted in animal biotechnology, bioethics, ES cells, iPS cells, Lasker Awards, livestock, Nobel Prize, transgenic research, web site | No Comments »

Transgenic mice obtained from androgenetic haploid embryonic stem cells

Wednesday, October 3rd, 2012
Transgenic mice obtained from androgenetic haploid embryonic stem cells (W Li et al. Nature 000, 1-5 (2012) doi:10.1038/nature11435)

Transgenic mice obtained from androgenetic haploid embryonic stem cells. Image adapted by permission from Macmillan Publishers Ltd:NATURE (W Li et al. Nature 000, 1-5 (2012) doi:10.1038/nature11435), copyright (2012).

Prof. Xiao-Yang Zhao (ISTT Ordinary member and awarded the first ISTT Young Investigator Award in Florida (USA), at the TT2011 meeting) and Prof. Qi Zhou (ISTT Honorary member and awarded the third ISTT Prize in Uppsala (Sweden), at the TT2004 meeting), and his colleagues from the State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, in Beijing (China), as well as other collaborating institutions, just published a letter in Nature describing how live transgenic mice can be obtained from androgenetic haploid embryonic stem cells (Li et al. Nature 2012).

Androgenetic haploid embryonic stem cells produce live transgenic mice
Wei Li, Ling Shuai, Haifeng Wan, Mingzhu Dong, Meng Wang, Lisi Sang, Chunjing Feng, Guan-Zheng Luo, Tianda Li, Xin Li, Libin Wang, Qin-Yuan Zheng, Chao Sheng, Hua-Jun Wu, Zhonghua Liu, Lei Liu, Liu Wang, Xiu-Jie Wang, Xiao-Yang Zhao & Qi Zhou
Nature (2012) doi:10.1038/nature11435.

Mouse androgenetic haploid embryonic stem (ahES) cells can be established by transferring sperm into an enucleated oocyte. These ahES cells maintain haploidy and are stable in culture. In addition, these ahES cells can contribute to the germline of chimeric mice when microinjected into blastocysts. Furthermore, these ahES cells can be delivered by intracytoplasmic injection into mature oocytes, eventually resulting into viable fertile mice that will inherit any genetic modification previously transferred to ahES cells while in culture. As stated by the authors, this work “provides a new approach for genetic manipulation in animal models without available germline-competent ES cells, including non-human primates, as modifications in such haploid stem cells could be transmitted to offspring through intracytoplasmic injection into mature oocytes, which may serve as a more efficient and simple strategy for gene-targeting studies“.

Earlier this year, in the April 27 issue of Cell, an independent study, developed in parallel, from Guo-Liang Xu’s (State Key Laboratory of Molecular Biology) and Jinsong Li‘s (State Key Laboratory of Cell Biology) laboratories from the Institute of Biochemistry and Cell Biology, Shanghai Institutes of Biological Sciences, Chinese Academy of Sciences in Shanghai (China) and their collaborators, including Xiang Gao (Model Animal Research Center, Nanjing University, Nanjing, China), reported similar extraordinary findings.

Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells.
Yang H, Shi L, Wang BA, Liang D, Zhong C, Liu W, Nie Y, Liu J, Zhao J, Gao X, Li D, Xu GL, Li J.
Cell. 2012 Apr 27;149(3):605-17.

These three scientists, Qi Zhou (Beijing, China), Jinsong Li (Shanghai, China) and Xiang Gao (Nanjing, China) will participate as invited speakers in the next 11th Transgenic Technology meeting (TT2013) that will be held in Guangzhou (China), on 25-27 February 2013. In particular, both Qi Zhou and Jinsong Li will be presenting their most recent and excellent works on the isolation of androgenetic haploid embryonic stem cells and their use to produce genetically-modified mice.

Once again, the International Society for Transgenic Technologies (ISTT) is pleased to present the latest advances in animal transgenic technology at the TT meetings, next one (TT2013) to be held in Guangzhou (China), in February 2013. Anyone interested in these transgenic techniques and their applications should not miss this great opportunity to learn all these new developments, directly presented by their inventors.

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Posted in animal biotechnology, ES cells, information, ISTT Prize, knock-outs, publication, transgenic research, TT meeting, web site, Young Investigator Award | No Comments »

The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

Sunday, September 23rd, 2012
The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The International Society for Transgenic Technologies (ISTT) was founded shortly after the Transgenic Technology (TT) meeting in Barcelona (TT2005). Since then, the ISTT family has been fortunate to visit several countries, every ~18 months. In 2007 we went to Brisbane (TT2007).  In 2008, we visited Toronto (TT2008). In 2010 we returned to Europe and held the TT2010 meeting in Berlin. For 2011 we visited the USA for the first time, and organized the TT2011 meeting in St Pete Beach (Florida). And, now, the next TT meeting (TT2013) is planned for China, in Guangzhou. This has been one of our aims and challenges, since the foundation of the ISTT, namely, holding a TT meeting in Asia, in China. Now it has become a reality. The 11th Transgenic Technology meeting (TT2013) will be held in Guangzhou (China), on 25-27 February 2013, organized by Prof. Ming Zhao (Chair) (Southern Medical University, Guangzhou) and his Organizing and Advisory Committees, immediately after celebrating the Chinese New Year of the Snake. More than 30 speakers have confirmed their participation, to discuss about ES cells, iPS cells, targeted nucleases (ZFNs and TALENs), cryopreservation and reproduction techniques, running a transgenic facility, mouse genetics, epigenetics, ethics and animal welfare, transgenesis in other vertebrates, animal models of disease, etc… among many other interesting topics. At the TT2013 meeting, the ISTT will award the 9th ISTT Prize for outstanding contributions to transgenic technologies to Prof. Allan Bradley,  Director Emeritus of the Wellcome Trust Sanger Institute (WTSI), in Hinxton (UK), and leader of the Mouse Genomics Team at WTSI.

In addition, a 3-day hands-on practical workshop (28 February-2 March 2013) will be offered in Guangzhou after the TT2013 meeting, addressing basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management and other interesting topics. This workshop is organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair,  Ming Zhao (Southern Medical University, Guangzhou, China), Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China).

Don’t miss this opportunity to hear the latest advances in animal transgenic by world experts! Time is going fast and first deadlines (15 October 2012), for registering at reduced fees, for submitting abstracts, for applying for ISTT registration awards and for nominating candidates for ISTT Young Investigator awards are rapidly approaching.

See you all in China!

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Posted in animal welfare, bioethics, course, cryopreservation, embryos, ES cells, facility, iPS cells, ISTT, ISTT Members, ISTT Prize, knock-outs, meetings, practical course, registration awards, rodent genetics, sperm, transgenic research, travel awards, TT meeting, web site, workshop, Young Investigator Award | No Comments »

Structural and Functional Concepts in Current Mouse Phenotyping and Archiving Facilities

Tuesday, September 18th, 2012
Structural and Functional Concepts in Current Mouse Phenotyping and Archiving Facilities

Structural and Functional Concepts in Current Mouse Phenotyping and Archiving Facilities

Anyone interested in building, modifying or renewing an animal (mouse) facility, with the aim of using it eventually for archiving (cryopreservation) and/or phenotyping purposes might find interesting reading the following article, which we prepared through the execution of the European Project INFRAFRONTIER.

Structural and Functional Concepts in Current Mouse Phenotyping and Archiving Facilities. Kollmus, Heike; Post, Rainer; Brielmeier, Markus; Fernández, Julia; Fuchs, Helmut; McKerlie, Colin; Montoliu, Lluis; Otaegui, Pedro J.; Rebelo, Manuel; Riedesel, Hermann; Ruberte, Jesús; Sedlacek, Radislav; de Angelis, Martin Hrabé; Schughart, Klaus . Journal of the American Association for Laboratory Animal Science, Volume 51, Number 4, July 2012 , pp. 418-435(18).

Abstract from JAALAS journal web page:

Collecting and analyzing available information on the building plans, concepts, and workflow from existing animal facilities is an essential prerequisite for most centers that are planning and designing the construction of a new animal experimental research unit. Here, we have collected and analyzed such information in the context of the European project Infrafrontier, which aims to develop a common European infrastructure for high-throughput systemic phenotyping, archiving, and dissemination of mouse models. A team of experts visited 9 research facilities and 3 commercial breeders in Europe, Canada, the United States, and Singapore. During the visits, detailed data of each facility were collected and subsequently represented in standardized floor plans and descriptive tables. These data showed that because the local needs of scientists and their projects, property issues, and national and regional laws require very specific solutions, a common strategy for the construction of such facilities does not exist. However, several basic concepts were apparent that can be described by standardized floor plans showing the principle functional units and their interconnection. Here, we provide detailed information of how individual facilities addressed their specific needs by using different concepts of connecting the principle units. Our analysis likely will be valuable to research centers that are planning to design new mouse phenotyping and archiving facilities.

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Posted in animal experimentation, cryopreservation, database, embryos, ES cells, facility, information, phenotyping, publication, web site | No Comments »

What are we talking about in the transgenic-list?

Friday, June 22nd, 2012
What are we talking about in the transgenic-list?

What are we talking about in the transgenic-list?

After 16 years and more than 20,000 messages posted one could ask: what are we talking about in the transgenic-list? What are the topics mostly discussed in this very popular list among people interested in animal transgenesis? One way to address this question is by using Wordle, a program that analyzes a text and extract the words that are mostly used and draws a graphic where the most frequent words appear more prominently.

Download a WORDLE for the transgenic-list (tg-l): small, big, huge.

What you see above is the result of providing Wordle with the subjects from these more than 20,000 messages posted through the tg-l over the past 16 years. Watching the resulting graphic provides a rather clear answer: we appear to be mostly talking about ES cells, mice, embryos, transgenic, injection, IVF, DNA, etc…  but clearly, ES cells is the most popular topic at the tg-l. Intreresting. A simple but very informative manner to present the usage of words in subjects of messages posted through the transgenic-list.

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Posted in embryos, ES cells, information, ISTT, tg-list, transgenic, transgenic research, web site | No Comments »

Registration for the TT2013 meeting in China is OPEN

Thursday, June 7th, 2012
Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

On behalf of the Organizing Committee for the 11th Transgenic Technology (TT2013) meeting and of the International Society for Transgenic Technologies (ISTT) it is my great pleasure to announce the launching of the official TT2013 meeting web site and, at the same time, inform that registration for the TT2013 meeting is now OPEN. After a very successful TT2011 meeting in Florida, the ISTT family travels to far-East, to China, to hold the 11th Transgenic Technology meeting in the city of Guangzhou, the largest city of the Guandong province in Southern China, on February 25-27, 2013. The TT2013 meeting is locally organized by the Southern Medical University, in Guangzhou, and the Chair of the Organizing Committee is Prof. Ming Zhao.

TT2013 meeting web site: http://www.tt2013.org

TT2013 meeting official email address: tt2013@transtechsociety.org

We welcome all attendees who are in the process of generating genetically-modified animals or who perform the experiments that follow to determine the corresponding phenotypes of such transgenic animals. The program should be of interest to scientists, group leaders, postdoctoral researchers, facility managers, technicians and PhD students working directly or indirectly with genetically-modified animals. We invite you to participate and contribute to this conference where we will discuss the latest technology developments, the newest applications and strategies in biology, biomedicine and biotechnology using transgenic animals. Companies and institutions with an interest in this field are kindly invited to participate and share their latest technologies. We encourage you to submit abstracts to be presented at the TT2013 meeting.

We have confirmed a wonderful list of excellent speakers and interesting topics for the animal transgenesis community. The list of invited speakers confirmed to attend the TT2013 meeting includes:

  • Fernando Benavides (MD Anderson Cancer Center, Smithville, TX, USA)
  • Allan Bradley (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK) ISTT Prize
  • James Bussell (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK)
  • Shannon Byers (The Jackson Laboratory, Bar Harbor, ME, USA)
  • Toni Cathomen (University Medical Center Freiburg, Germany)
  • Alan Colman (Institute of Medical Biology, Singapore) Closing Talk
  • Michael Dobbie (Australian Phenomics Facility, The Australian National University, Canberra, Australia)
  • Scott Fahrenkrug (Recombinetics, Minneapolis, MN, USA)
  • Malcolm France (Sydney University, Sydney, Australia)
  • Xiang Gao (Model Animal Research Center of Nanjing University, Nanjing, PR China)
  • Alfonso Gutiérrez-Adán (Dep. Animal Reproduction, INIA, Madrid, Spain)
  • Yann Herault (Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France)
  • Benoît Kanzler (Max-Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany)
  • Dietmar Kappes (Fox Chase Cancer Center, Philadelphia, PA, USA)
  • Takashi Kohda (Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan)
  • Thomas Kolbe (Biomodels Austria and Institute for Biotechnology in Animal Production, IFA-Tulln, Austria)
  • Takashi Kuramoto (Institute of Laboratory Animals, Kyoto University, Kyoto, Japan)
  • Liangxue Lai (Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, PR China)
  • Jinsong Li (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Ning Li (State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing, PR China)
  • Kent Lloyd (University of California, Davis, CA, USA)
  • Shoukhrat Mitalipov (Oregon National Primate Research Center, OHSU, Beaverton, OR, USA)
  • Naomi Nakagata (Center for Animal Resources and Development, Kumamoto University, Japan)
  • Catheryn O’Brien (The Walter and Eliza Hall Institute, Melbourne, Australia)
  • Masaru Okabe (Genome Information Research Center Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan)
  • Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA)
  • Xin-an Pu (The Ohio State University, Comprehensive Cancer Center, Columbus, OH, USA)
  • Ling Sun (Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, PR China)
  • Davor Solter (Institute of Medical Biology, Singapore) Opening Talk
  • Zhu-Gang Wang (Shanghai Research Center for Model Organisms, Shanghai, PR China)
  • Guoliang Xu (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Bo Zhang (College of Life Sciences, Peking University, Beijing, PR China)
  • Qi Zhou (The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)

The list of topics that will be covered at the TT2013 meeting includes the following themes:

  • Androgenetic haploid embryonic stem cells
  • Sperm Cryopreservation and IVF
  • KOMP ES cell clones performance
  • Australian Phenomics Network Initiatives
  • Epigenetics and Transgenesis
  • Effects of in vitro culture on mammalian embryos
  • Epigenetic alterations during mouse transgenesis and cloning
  • Epigenetic reprogramming by oocytes
  • Targeted nucleases, ZFNs and TALENs, in transgenesis
  • Livestock Genome Editing with TALENS
  • Transgenic mice and ZFNs
  • KO pigs and ZFNs
  • Zebrafish and TALENs
  • Transgenic livestock
  • Ethics, animal welfare and regulations
  • Health monitoring protocols and transgenic facilities
  • Animal welfare and international mouse consortia
  • Impact of genetic background in mouse and rat models
  • Assisted reproduction strategies in mice
  • Non-rodent transgenesis
  • Primate chimeras and ES cells
  • Rat functional genomic initiatives
  • National BioResource Project for the rat
  • Animal models of human diseases
  • ES and iPS cells
  • transposon (PiggyBac)-mediated mutagenesis
  • Round-table discussion: Running a transgenic facility
  • Running a transgenic facility: The business aspect of running a transgenic unit
  • Running a transgenic facility: managing errors, difficulties
  • Running a transgenic facility: Staying on the cutting edge of technology while still maintaining basic services
  • Rat/mouse chimeras and iPS/ES cells
  • Overview of cre-transgenic mouse lines resources
  • China/Asia/Oceania in the International Mouse Functional Genomics Consortia
  • The Shanghai Research Center for Model Organisms

The TT2013 meeting will be held at the Baiyun International Convention Center-Oriental International Convention Hotel in Guangzhou, the largest comprehensive five-star hotel in Southern China, with an established record of hosting successful meetings and perfect supporting facilities. Located on the Baiyun Mountain, in the Baiyun New Town and overlooking the Baiyun Mountain Scenic Area and the Oriental Resort, this Convention Center is a fully equipped, self-contained meeting resort. More than 40 international air routes serve the new Baiyun International Airport, a 20 minute drive from the Baiyun International Convention Center.

After the TT2013 meeting, there will be a 3-days hands-on practical workshop on transgenic techniques, organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair; Ming Zhao (Southern Medical University, Guangzhou, China); Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China). This workshop is limited to 30 participants. Instructors for the practical course will include: Wenhao Xu (USA), Xin-an Pu (USA), Anna Auerbach (USA), Lily Reed (USA), Xin Rairdan (USA), Barbara Stone (USA), Steven Xing (USA), Margaret Landis (USA), Katja Becker (Germany), Ronald Naumann (Germany). Techniques that will be discussed and demonstrated at this course include: basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management,… This practical course will be co-sponsored by Hamilton-Thorne, ParaTechs, Sutter/Primetech. Registration for this 3-days practical workshop must be done through tt2013@transtechsociety.org and the TT2013 meeting registration web page. Workshop cost: 400$ (USD).

Registration fee to attend the TT2013 meeting begins at 400$(USD) and extends up to 630$(USD). ISTT members and technician/student participants are entitled to reduced registration fees.

Accommodation is NOT included in the registration fee and lodging must be reserved independently at the Guangzhou Baiyun International Convention Center, where a limited number of rooms have been prebooked for participants attending the TT2013 meeting at convenient reduced prices (610 RMB-single/680 RMB-double room). Note: RMB are China Yuan Renminbi, 100 RMB ~ 16 USD/12 Euros. Please check for most updated currency exchange.

The International Society for Transgenic Technologies (ISTT) will be sponsoring several Registration Awards for ISTT members willing to attend the TT2013 meeting. Applications for ISTT Registration Awards to attend the TT2013 meeting will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Registration Awards.

The International Society for Transgenic Technologies (ISTT), in collaboration with inGenious Targeting Laboratory (iTL), has establised the ISTT YOUNG INVESTIGATOR AWARDthat will be given at the Transgenic Technology (TT) Meetings. Nominations for the 2nd ISTT Young Investigator Award, for the TT2013 meeting, will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Young Investigator Awards.

Sponsoring companies willing to support the TT2013 meeting and to exhibit at the TT2013 meeting venue should contact TT2013 organizers through the TT2013 meeting email address (tt2013@transtechsociety.org). TT2013 meeting sponsorship opportunities are available at the meeting web site.

TT2013 meeting deadlines

Abstract submission deadline:  October 15, 2012
Application for ISTT registration awards:  October 15, 2012
Awards to be communicated by October 30, 2012
Early bird registration deadline: October 15, 2012
Standard fee registration deadline: January 31, 2013
Late registration fees & on-site registration: February 01, 2013

See you all in Guangzhou, at the TT2013 meeting!

Lluis Montoliu, PhD; President of the ISTT

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Posted in animal biotechnology, animal experimentation, co-sponsorhip, course, embryos, ES cells, general, information, iPS cells, ISTT, ISTT booth, ISTT Members, ISTT Prize, knock-outs, knockout, meetings, practical course, registration awards, rodent genetics, sperm, transgenic research, travel awards, TT meeting, web site, workshop, Young Investigator Award | No Comments »

EMMA Cryopreservation Workshop in Madrid: a meeting report

Friday, May 11th, 2012
EMMA Cryopreservation Workshop: a meeting report

EMMA Cryopreservation Workshop: a meeting report

The EMMA (European Mouse Mutant Archive) Cryopreservation Workshop took place earlier this week in Madrid, May 7-8, at the main campus of CSIC, with an excellent success, organized by EMMA, supported by the EC-7th Framework Programme and co-sponsored by the International Society for Transgenic Technologies (ISTT). Sixty participants from many countries around the world gathered to present and discuss, in depth, the latest approaches, methodologies and techniques available for the efficient cryopreservation of mouse strains, through embryo, sperm and ovary cryopreservation. In addition to invited speakers and invited participants, the workshop was attended by delegates from EMMA nodes and 12 ISTT members. As many as 21 talks were delivered, by selected invited speakers, representing the different major archiving iniatives currently existing (EMMA, MMRRC, The Jackson Laboratory, RIKEN, APN, CMMR, AMMRA) and the research and development initiatives, as well as state-of-art protocols in the field. Presentations, abstracts and pictures from this EMMA Cryopreservation Workshop are freely available to anyone interested, from the meeting web site, and can be also accessed from ISTT and EMMA web sites. The remaining presentations will be progressively added upon receiving the approval from the corresponding guest speakers.

At EMMA, we envisaged this cryopreservation workshop as a forum to brainstorm and discuss in depth the latest technological advances in cryopreservation, including sperm and embryo cryopreservation, updated IVF methods and related techniques as ovary cryopreservation, laser-assisted and piezo-driven ICSI, transportation of frozen material and other technical and logistic challenges relevant to the operation of current mouse embryo/sperm archives. We believed we entirely fulfilled the expectations and all participants went back home, to their research institutions, loaded with new ideas, updated solutions and suggested improvements that can be explored and applied for a most efficient management of a mouse embryo/sperm cryopreservation bank. All participants agreed to continue organizing this type of focused workshops in the near future. The ISTT will be always there, ready to support these very interesting initiatives.

Lluis Montoliu, EMMA Spanish node

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Posted in co-sponsorhip, cryopreservation, database, embryos, ES cells, facility, ISTT, knock-outs, knockout, Meeting report, rodent genetics, service, sperm, transgenic research, web site, workshop | No Comments »

The TT2013 meeting in China: progress and updated information

Friday, April 27th, 2012
The TT2013 meeting in China: progress and updated information

The TT2013 meeting in China: progress and updated information

The organization of the 11th Transgenic Technology meeting (the TT2013 meeting), to be held in Guangzhou, PR China, on February 25-27, 2013,  is progressing well. Prof. Ming Zhao (Southern Medical University in Guangzhou), Chair of the TT2013 Organization, and all his colleagues in the various advisory and supporting committees, are doing a great job and, hence, from the International Society for Transgenic Technologies (ISTT) we are sure that the TT2013 meeting is going to be yet another great transgenic conference for all of us to learn, discuss and enjoy.  The preliminary TT2013 meeting web page already informs about the confirmed topics and speakers that will be in Guangzhou. These will be regularly updated as we receive the confirmations from all invited speakers. Registration is expected to be open in June. The registration scheme and registration fees will be similar to the previous TT2011 meeting. The TT2013 meeting will be followed by a 3-day practical hands-on course on basic techniques in transgenesis, coordinated by Dr. Wenhao Xu (University of Virginia, Charlottesville, VA, USA).

Currently, the confirmed topics and speakers attending the TT2013 meeting include:

Meeting Topics

  •  Sperm Cryopreservation and IVF
  • KOMP ES cell clones performance
  • Epigenetics and Transgenesis
  • Effects of in vitro culture on mammalian embryos
  • Targeted nucleases, ZFNs and TALENs, in transgenesis
  • Transgenic pigs and TALENs
  • Transgenic mice and ZFNs
  • KO pigs and ZFNs
  • Zebrafish and TALENs
  • Ethics, animal welfare and regulations
  • Health monitoring protocols and transgenic facilities
  • Non-rodent transgenesis
  • Primate chimeras and ES cells
  • Rat functional genomic initiatives
  • Animal models of human diseases
  • ES and iPS cells
  • transposon (PiggyBac)-mediated mutagenesis
  • Round-table discussion: Running a transgenic facility
  • Running a transgenic facility: The business aspect of running a transgenic unit
  • Running a transgenic facility: managing errors, difficulties
  • Running a transgenic facility: Staying on the cutting edge of technology while still maintaining basic services
  • Rat/mouse chimeras and iPS/ES cells
  • Overview of cre-transgenic mouse lines resources
  • China/Asia/Oceania in the International Mouse Functional Genomics Consortia
  •  3-days hands-on practical workshop on transgenesis procedures after the TT2013 meeting
  • ..

Confirmed Speakers

  • Allan Bradley (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK)
  • Alan Colman (Institute of Medical Biology, Singapore)
  • Scott Fahrenkrug (Recombinetics, Minneapolis, MN, USA)
  • Malcolm France (Sydney University, Sydney, Australia)
  • Xiang Gao (Model Animal Research Center of Nanjing University, Nanjing, PR China)
  • Alfonso Gutiérrez-Adán (Dep. Animal Reproduction, INIA, Madrid, Spain)
  • Yann Herault (Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France)
  • Dietmar Kappes (Fox Chase Cancer Center, Philadelphia, PA, USA)
  • Liangxue Lai (Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, PR China)
  • Kent Lloyd (University of California, Davis, CA, USA)
  • Shoukhrat Mitalipov (Oregon National Primate Research Center, OHSU, Beaverton, OR, USA)
  • Naomi Nakagata (Center for Animal Resources and Development, Kumamoto University, Japan)
  • Catheryn O’Brien (The Walter and Eliza Hall Institute, Melbourne, Australia)
  • Masaru Okabe (Genome Information Research Center Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan)
  • Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA)
  • Ling Sun (Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, PR China)
  • Bo Zhang (College of Life Sciences, Peking University, Beijing, PR China)
  • Qi Zhou (The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)

From the ISTT, as in previous TT meetings, we will also promote and support the participation of ISTT members by sponsoring several registration awards for ISTT Members. Deadline for submitting applications for these ISTT Registration Awards is October 15, 2012. Instructions to apply can be found at the corresponding web page of the ISTT web site.

Please, write down the dates of the TT2013 meeting: February 25-27, 2013, and make sure you don’t miss this conference!. See you all in China next year!

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Posted in animal experimentation, animal welfare, bioethics, course, cryopreservation, embryos, ES cells, facility, genes, iPS cells, ISTT, ISTT Members, ISTT Prize, meetings, practical course, registration awards, transgenic, transgenic research, TT meeting, workshop | No Comments »

World Map of Transgenic Core Facilities

Saturday, April 7th, 2012
World Map of Transgenic Core Facilities

World Map of Transgenic Core Facilities

At the International Society for Transgenic Technologies (ISTT) web site, according to the aims of our Society. we care to provide the entire scientific community with as much information as possible regarding how and where to generate genetically modified animals, particularly transgenic and knockout mice, as useful animal models for research projects in biology, biotechnology and biomedicine. One of these resources of information is the World Map of Transgenic Core Facilities, currently holding more than 125 links to web sites of transgenic core facilities located in 27 countries, world wide. The transgenic core facilities can be easily found in a list, arranged per country, or using a useful Google Maps built-in feature depicting the geographic location of each transgenic facility.

Is your transgenic core facility not yet listed in the World Map of Transgenic Core Facilities offered from the ISTT web site? No problem. Whether public or private, whether based on an academic environment or associated with a company, all transgenic core facilities, all initiatives meant to produce transgenic animals (mice, rats, other mammals, other vertebrates,…) on demand, for research purposes, are welcome and we, at the ISTT, will be pleased to include all these links in our web site. Please contact us at istt@transtechsociety.org and send us your web link and contact details of your transgenic core facility and we will be more than happy to add your transgenic core facility to the list of World Map of Transgenic Core Facilities.

Thanks for submitting the web site of your Transgenic Core Facility to the ISTT.

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Posted in database, embryos, ES cells, facility, general, genes, information, knock-outs, knockout, list, rodent genetics, transgenic, transgenic research, web site | No Comments »

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