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ISTT Registration Awards for TT2016 meeting in Prague

Monday, July 27th, 2015
ISTT Registration Awards for TT2016 meeting in Prague

ISTT Registration Awards for TT2016 meeting in Prague

A minimum of six Registration Awards will be sponsored by the International Society for Transgenic Technologies for ISTT members wishing to attend the 13th Transgenic Technologies (TT2016) meeting in Prague, The Czech Republic, on 20-23rd March, 2016. Selected applicants will be awarded funding to cover registration fees plus attendance at all social events. However, the award does not cover travel expenses, hotel accommodation or attendance at pre-meeting events.

Applicants who are not yet members of the ISTT may join the ISTT and simultaneously submit their Registration Award application. Only those applications from members who have paid their current annual fees will be considered.

Applicants must register first at the TT2016 Meeting website and select, as a payment method, “Application for Registration Awards” as a payment method. The ISTT will pay the Registration Fee of all applicants selected for an award. Applicants not selected will be kindly requested to pay the corresponding registration fee.
Applications and additional required documents (see below) should be sent, along with the meeting registration confirmation, to the official ISTT email address,, by 30th November, 2015.

Additional Documentation Required
a) Applicant’s CV
b) For ordinary members, a letter from the applicant describing how he/she will benefit from attending the TT meeting
c) For technician/student members, a letter of support from the applicant’s PI or supervisor stating how attendance will benefit the applicant’s career

Selection Process
ISTT Registration awards will be selected by a subset of ISTT Council members, with preference given to:
1) Student/Technician ISTT Members
2) ISTT Members submitting an abstract for presentation as a poster/short-oral communication at the TT meeting
3) Any other ISTT Member

Awards to the selected applicants will be announced by December 15th, 2015 and awardees will receive a diploma marking the event at the end of the TT2016 Meeting.

Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

Saturday, July 4th, 2015
Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

Dear colleagues,

we are pleased to announce that registration and abstract submission for the 13th Transgenic Technology Meeting (TT2016) in Prague, Czech Republic from 20th – 23rd March 2016 are now OPEN.

Andras Nagy will give the opening plenary lecture and Denis Duboule the closing one.
Thomas Boehm and Richard Behringer will give our special Orbis pictus lectures.

Further information can be found at the conference website.

We look forward to seeing you here in the beautiful city of Prague.

Radislav Sedlacek
Chair of the TT2016 Organizing Committee

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015)

Friday, May 22nd, 2015
Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015), 11-12 June 2015, in Leiden, the Netherlands

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015), 11-12 June 2015, in Leiden, the Netherlands

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015) that will be held on 11-12 June 2015, in Leiden, the Netherlands. The popular biannual “Workshop on Innovative Mouse Models” brings together a diverse group of scientists interested in developing and exploiting mouse models to study fundamental developmental processes and to mimic human disease. Keynote speakers from leading laboratories present the latest developments on advanced genome alteration protocols, this year specifically focusing on the use of CRISPR/Cas9-assisted gene modification. Also novel imaging-technologies will be presented. The two-day workshop format combines keynote lectures and presentations of selected abstracts in order to encourage in-depth and unvarnished discussions of novel technologies.
For more information, scientific programme with confirmed speakers and registration, please visit:
Looking forward to meet many of you at IMM2015 in Leiden!

ISTT Awarded Scientists

Friday, February 27th, 2015
ISTT Awarded Scientists (2001-2014). Outstanding researchers in the field of Transgenic Technologies that have been awarded the ISTT Prize or the ISTT Young Investigator Award

ISTT Awarded Scientists (2001-2014). Outstanding researchers in the field of Transgenic Technologies that have been awarded the ISTT Prize or the ISTT Young Investigator Award

The International Society for Transgenic Technologies (ISTT) has updated its web site and now includes, in its public pages, additional information for all the awarded scientists, outstanding researchers in the field of Transgenic Technologies, which have been awarded the ISTT Prize, generously sponsored by genOway, or the ISTT Young Investigador Award, generously sponsored by ingenious targeting laboratory.

These are the 10 outstanding scientists awarded the ISTT Prize for the period 2001-2014. The ISTT was founded in 2006 and the first ISTT Prize was awarded in Toronto, at the TT2008 meeting. Previously, these awards were known as the genOway Prize for transgenic technologies.

  • Janet Rossant, 10th ISTT Prize, TT2014 meeting, Edinburgh, UK
  • Allan Bradley, 9th ISTT Prize, TT2013 meeting, Guangzhou, China
  • Ralph L. Brinster, 8th ISTT Prize, TT2011 meeting, Florida, USA
  • A. Francis Stewart, 7th ISTT Prize, TT2010 meeting, Berlin, Germany
  • Brigid Hogan, 6th ISTT Prize, TT2008 meeting, Toronto, Canada
  • Charles Babinet, 5th genOway Prize, TT2007 meeting, Brisbane, Australia
  • Andras Nagy, 4th genOway Prize, TT2005 meeting, Barcelona, Spain
  • Qi Zhou, 3th genOway Prize, TT2004 meeting, Uppsala, Sweden
  • Kenneth J. McCreath, 2nd genOway Prize, TT2002 meeting, Munich, Germany
  • Teruhiko Wakayama, 1st genOway Prize, TT2001 meeting, Stockholm, Sweden

These are the 3 outstanding researchers awarded the ISTT Young Investigator Award for the period 2011-2014

  • Feng Zhang, 3rd ISTT Young Investigator Award, TT2014 meeting, Edinburgh, UK
  • Toru Takeo, 2nd ISTT Young Investigator Award, TT2013 meeting, Guangzhou, China
  • Xiao-Yang Zhao, 1st ISTT Young Investigator Award, TT2011 meeting, Florida, USA

SALAAM kick-off meeting in Munich: thinking big (the important role of large animal models)

Monday, January 19th, 2015
SALAAM kick-off meeting in Munich: 15-17 December 2014

SALAAM kick-off meeting in Munich: 15-17 December 2014

About a month ago, shortly before the season break, and very timely to enjoy its Christmas Market (Weihnachtsmarkt), the kick-off meeting of the Project SALAAM (Sharing Advances on Large Animal Models) took place in Munich (Germany), 15-17 December 2014, beautifully organized by Eckhard Wolf and Pascale Chavatte-Palmer, Chair and Co-Chair of this EU-COST Action BM1308. This conference, open to any interested researcher in the field, represented the official launch of the SALAAM project, to discuss about the role of large animal models in Translational Medicine, “Bridging the Gap between Basic and Clinical Research”, as indicated in the SALAAM logo. During these three days, about 120 scientists, including researchers not initially associated with SALAAM (including several ISTT members), gathered at the Gene Center, LMU Munich, to share their views about the role of large animal models in biomedicine.

The meeting started with a welcome address by Eckhard Wolf (LMU, Munich, Chair of SALAAM) who set the stage and underlined the need to use appropriate animal models for succeeding in translational research. In the past, large amount of resources have been devoted to rodents, mostly mice, in biomedicine, where mouse models have become instrumental for the current understanding of how most of our genes work and greatly facilitated the progress in the post-genomic era. However, in spite of mice being widely used in Biomedicine to model human diseases, often mice fail to accurately reproduce the features associated with a given human pathology. Therefore there is an urgent need to develop non-rodent animal models that would mimic aspects of human anatomy and human physiology more closely. Pigs, small ruminants and rabbits appear to be excellent candidates to follow up the preliminary discoveries made in mice, and they are the main purpose of the SALAAM initiative, through all the appointed participants, experts in these large animal models. The conference continued for its first day with lectures by A. Aartsma-Rus (NL), and S. Wildhirt (DE), who described examples of use of large animal models for Duchenne muscular dystrophy and for the development of medical devices, respectively. The initial Ethical perspective on the use of large animals was provided by N. Stingelin (CH). This first day concluded with an interesting key-note lecture by M.M. Mohiuddin (USA) on the recent advances in pig-to-primate cardiac xenotransplantation.

On the second day, the conference presented the very large repertoire of methods and techniques that are currently available for Genetic Tailoring of large animal models. Angelika Schnieke (DE) introduced the state of art for the current genetic engineering of large animals, nicely summarizing many years of techniques and developments that have been successfully applied for the production of large genetically modified animal models. This initial talk was followed by a presentation by Lluis Montoliu (ES) on the use of CRISPR-Cas9 approaches to functionally analyze the role of non-coding genomic sequences, illustrated with some examples tested in mice, depicting the important role of rodents in proof-of-concept type of experiments, before undertaking subsequent experiments in larger animal models. B. Grzeskowiak (PL) presented an innovative set of nanomagnetic gene delivery vectors for transgenesis. Two additional talks illustrated the power of genetic engineering of the pig genome, using transposons (W.A. Kues, DE) or very elaborated gene cassettes for regulating and tracing disease genes (J.E. Jakobsen, DK). The session ended with a presentation from goats, where L. Boulanger (FR) reported the role of FOXL2 as a female sex-determining gene.

The SALAAM conference continued with a session devoted to systematic phenotyping initiatives of large animal models. At first, H. Fuchs (DE), presented the experience and phenotyping pipeline of the German Mouse Clinic, operating within the Infrafrontier consortium, and a good example of successful systematic phenotyping in mice. Next, Pascale Chavatte-Palmer (FR) discussed the achievements and challenges of imaging techniques in large animal models, through her studies on reproduction and fetal development. J. Tibau (ES) presented his interesting studies using pigs to analyze human obesity and to validate the effect of diets on the evolution of fat deposition using tomography approaches. A. Blutke (DE) introduced the impressive Munich MIDY-PIG Biobank initiative, as a unique resource for translational diabetes research. The two last talks presented the use of pigs as models for respiratory infections (K. Skovgaard, DK) or cystic fibrosis (I. Caballero, FR).

The last standard session of this SALAAM conference was devoted to discuss how to select the best animal model. This session began with an interesting presentation by J. Langermans (NL), who shared their initiative of non-human primate biobanking for translational medicine, a collaborative consortium where most of the nonhuman primate research centres in Europe were represented. He also discussed the unique features of non-human primates to investigate devastating diseases affecting us, such as the new infections (i.e. Ebola) or neurodegenerative diseases (i.e. Alzheimer, Parkinson) , often very challenging to be reproduced in non-primate animal models. Next, Antonio Gonzalez-Bulnes (ES) discussed the advantages and challenges of using pigs and sheep animal models, whereas L. Hiripi (HU) presented the unique features of the rabbit models.  V. Huygelen (BE) discussed the use of piglets to investigate the human low birth weight cases , and A. Navarrete Santos (DE) further presented rabbits as ideal models for investigating diabetes during pregnancy. Diabetes research was also the focus of the last speaker of the session, G. Pennarossa (IT), whose experimental dessigns are focused on the use of dogs to explore cell therapy-based treatments.

The SALAAM first public conference ended with an excellent and very motivating talk by Karin Blumer (CH) on the ethical aspects of using large animals. She challenged the audience with the question whether “size did matter?” when it comes to Ethics and Animal Models. Her presentation nicely illustrated the different Ethical perspectives existing in the field and, most importantly, the relevant parameters that should be taken into account in order to properly address this question. She presented the “size” of an animal as an accidental attribute, not an intrinsic value, that must not determine its moral status. This presentation triggered an interesting and live discussion among the participants.

On the third and last day, the different working groups of SALAAM gathered first independently to discuss the next initiatives and eventually shared their conclusions in a combined general session. The planned initiatives will include the organization of practical workshops on CRISPR-Cas9 and transposon technologies, the generation of specific pig Cre-transgenic lines for the production of conditional pig mutant animal models,  the need to standardize phenotyping protocols associated with additional specific training courses, the preparation of biobanks and associated databases for archiving and sharing tissues from large animal models, and the creation of a group to analyze the implementation of the 2010/63/EU Directive across Europe, the public perception and ethical issues of animal research, and the need for training to adequately communicate results to the public.

Information about future plans, initiatives and activities of the SALAAM EU-COST action will be available from its dedicated web site.

Job announcement: ISTT Administrative Assistant

Thursday, November 27th, 2014
Job announcement: ISTT Administrative Assistant

Job announcement: ISTT Administrative Assistant

Job Title: Administrative Assistant

About ISTT: The International Society for Transgenic Technologies (ISTT) was founded in 2006, and is registered in Buffalo NY, USA, as a not for profit (NFP) organization. It currently represents an enthusiastic group of more than 700 members worldwide. We seek to foster communication and technology-sharing to enhance scientific research and to advance the field of animal transgenesis, particularly as it applies to experimental models in science. We represent the interests of scientists as well as technicians working in the field of transgenic technologies. The ISTT sponsors a series of periodic international transgenic technology (TT) meetings.

Job Description: This is a part-time job, may be conducted from home, and offers the candidate great flexibility. It is expected to require an average of 25 hours per week. Workloads may be considerably higher during the peak period of membership renewals and during the organization of the periodic TT meetings. The candidate must be able to travel to TT meetings, which are hosted at various venues worldwide every 18 months. The Society will provide the candidate with a laptop, support for Internet access, and a mobile phone to conduct Society business.

Job Specific Responsibilities: Administrative assistant position. Duties will include:
• Assist the ISTT President in reporting activities and other general administrative tasks.
• Assist the ISTT Treasurer with tracking of incomes and expenses.
• Act as a central contact point for all ISTT stakeholders.
• Register new members and renew existing memberships.
• Maintain current contact information of all members.
• Support the organization of the global meetings.
• Attend TT meetings to help represent the ISTT and help with administrative duties – meetings are all over the world and require travelling.
• Assist the ISTT Secretary with the preparation of minutes of ISTT General Assemblies and ISTT Board meetings.
• Monitor ISTT contracts with sponsors.
• Maintain an ISTT agenda and send reminders for upcoming deadlines to ISTT members (e.g. membership renewals, registrations, etc.)

Candidate Requirements: 2+ years experience as an Administrative Assistant. Independent, self-starter with strong organizational skills. [Alternative experience demonstrating administrative capability will be considered.] Excellent time management and ability to manage deadlines. Fluent English – Strong verbal and written communication skills. Experience in identifying and fulfilling NFP society needs. Experience with project management tools, including but not limited to: MS Excel, MS Word, MS Project, MS PowerPoint. Webmaster and content management not a must but a plus.

Preferred Qualifications: Bachelor’s Degree preferred

Country: United States of America

City: Work from Home, ideally close to Houston, Texas, for direct exchange with the president of the society.

Primary Location: WORK FROM HOME (occasional meetings at Texas Medical Center, Houston, TX)

Job Function: Office Administration

Worker Type: Regular Employee

Full/Part Time: Part-Time

Shift Type: flexible

Hours per Week: Minimum of 25 hours/week

Work Days: Monday – Friday (very occasional weekend work)

Posting Currency: USD

Posting Range: $20,000 – $25,000 (salary commensurate with experience). All required travel expenses covered by the society

Application: Interested candidates should submit their CVs and references to


The TT2014 meeting Scottish style

Tuesday, October 14th, 2014
The TT2014 meeting at the Assembly Rooms, Edinburgh, Scotland, UK, 6-8 October 2014

The TT2014 meeting at the Assembly Rooms, Edinburgh, Scotland, UK, 6-8 October 2014

The most popular ISTT meeting held to date took place last week in Edinburgh, UK, at the Assembly Rooms. More than 530 participants gathered in Scotland for the 12th Transgenic Technology (TT2014) meeting during three days, 6 to 8 October 2014, to discuss the latest advances in methods and applications for generating and analyzing genetically modified animals. The local Organizers, Douglas Strathdee, Peter Hohenstein and Bruce Whitelaw showed us how to run an splendid TT meeting Scottish style.  The Organizing committee, helped by the In-Conference team and the ISTT council and Administration, should be praised for putting together an excellent collection of talks, covering the most up-to-date topics, where all participants could learn and share their experiences.

Janet Rossant awarded the 10th ISTT Prize at the TT2014 meeting in Edinburgh, for her outstanding contributions to the field of transgenic technologies. Janet Rossant appears in this picture with Alexandre Fraichard (left, genOway, CEO, sponsor of this award) and Lluis Montoliu (past-ISTT President, right)

Janet Rossant awarded the 10th ISTT Prize at the TT2014 meeting in Edinburgh, for her outstanding contributions to the field of transgenic technologies. Janet Rossant appears in this picture with Alexandre Fraichard (left, genOway, CEO, sponsor of this award) and Lluis Montoliu (past-ISTT President, right)

As many as 54 speakers were invited to present their research, including two keynote addresses (Rudolf Jaenisch, Alexandra Joyner), at the opening and clossing of the TT2014 meeting, two ISTT awarded scientists (Janet Rossant-10th ISTT Prize- and Feng Zhang-3rd ISTT Young Investigator Award), 26 invited speakers, 6 Showcase presentations from Scottish scientists, 5 short talks and 15 teasers selected from Poster abstracts. The impressive numbers of the TT2014 meeting continue with 176 Posters presented by participants attending the conference in Edinburgh and the many sponsors and exhibitors that generously contributed to the success of the 12th Transgenic Technology Meeting. The ISTT conference was followed by a hands-on workshop on basic zebrafish transgenic methods.

Lluis Montoliu, founder and past-ISTT President, awarded a sculpture representing a zygote, created by Bela Rozsnyay, by ISTT Board members for his dedicated work for the progress of the ISTT since inception of the Society and during the past 9 years (2006-2014). Lluis Montoliu appears next to Alison Cameron (ISTT Administrative Assistant) and Aimee Stablewski (ISTT Treasurer and Secretary)

Lluis Montoliu, founder and past-ISTT President, awarded a sculpture representing a zygote, created by Bela Rozsnyay, by ISTT Board members for his dedicated work for the progress of the ISTT since inception of the Society and during the past 9 years (2006-2014). Lluis Montoliu appears next to Alison Cameron (ISTT Administrative Assistant) and Aimee Stablewski (ISTT Treasurer and Secretary)

During the TT2014 meeting, at the corresponding ISTT General Assembly, the ISTT Members approved to dissolve the legal entity representing the Society in Spain, which has been substituted by the new legal entity, ISTT, Inc., a not-for-profit association registered in the New York State (USA) that from now onwards will be representing the interests of the Society. Also, during the course of the ISTT conference in Edinburgh, the ISTT Board members awarded Lluis Montoliu (Founder and Past-President of the ISTT, 2006-2014) and thanked his dedicated work for the ISTT, since its inception. Jan Parker-Thornburg is the new President of the ISTT.

The 13th Transgenic Technology Meeting, TT2016, will be held in Prague (Czech Republic) on 20-23 March 2016, organized by Radislav Sedlacek

The 13th Transgenic Technology Meeting, TT2016, will be held in Prague (Czech Republic) on 20-23 March 2016, organized by Radislav Sedlacek

The conference in Edinburgh concluded with the warm invitation by Radislav Sedlacek (IMG, Prague, Czech Republic), Organizer of the 13th Transgenic Technology Meeting, TT2016, to attend the next ISTT conference that will be held in Prague, Czech Republic, on 20-23 March 2016.

Good bye and thanks to Edinburgh! Hello and see you all again in Prague!

Welcome to Edinburgh, the city hosting the TT2014 meeting!

Friday, October 3rd, 2014
Welcome to Edinburgh, the city hosting the TT2014 meeting!

Welcome to Edinburgh, the city hosting the TT2014 meeting!

The 12th Transgenic Technology Meeting is about to begin this forthcoming weekend. On Sunday, 5 October, the Organizers have nicely prepared a welcome pre-meeting dinner to get together and meet all colleagues attending. The real TT2014 meeting‘s kick off will be on Monday morning, 6 October. During the following three days all delegates will have the pleasure (and the privilege, owing the great interest triggered by this conference, absolutely sold out) to listen and discuss the latest advances in transgenic technologies, through lectures delivered by the key players in our field, the ones that have developed or contributed to disseminate the use of the newest tools, the most updated experimental strategies applied to generate and analyze genetically modified animals. More than 530 participants will fill the Assembly Rooms in the historic city of Edinburgh. This TT meeting has become not only the most popular ISTT conference organized to date but also the TT meeting where the highest number of posters (176) will be shown. Therefore, besides enjoying the talks, all TT2014 delegates will have a unique opportunity to browse through the work of dozens of many other laboratories and facilities, willing to share their advances, problems encountered, solutions found and innovative proposals.

The International Society for Transgenic Technologies (ISTT) is proud to have chosen Edinburgh as the venue for TT2014 and, hence, wants to congratulate the Organizing Team, led by Douglas Strathdee, Chair of the conference, along with Peter Hohenstein and Bruce Whitelaw, co-chairs, the technical secretariat provided by In-Conference and the rest of members of the Organizing committees, for having prepared what surely will be another great TT meeting, as all precedent meetings were. The TT2014 meeting will be followed by a practical workshop on Zebrafish Transgenic Techniques, where a limited number of participants will have the opportunity to meet experts in the field and learn the basic techniques applied nowadays to use this vertebrate as an alternative animal model to study gene function or disease.

If you are already in Edinburgh, enjoy the city! If you are travelling to Edinburgh: have a safe trip! If, however, and unfortunately, you have not managed to attend this time, but you are a member of the ISTT, please wait to grasp some of the excellence and talent that will be presented during next week through the talks that we will be at the members-only area, after obtaining the consent from the corresponding speakers. Also, life goes on, and ISTT is already planning for the next TT meeting, already launched: the 13th Transgenic Technology Meeting (TT2016) that will be held in Prague (Czech Republic) in March 2016, organized by Radislav Sedlacek.

See you all soon in Edinburgh!


CARD-RPCI Cryopreservation Course Report

Monday, September 22nd, 2014
CARD-RPCI Cryopreservation Course Report

CARD-RPCI Cryopreservation Course Report

During the past week, 15-19 September 2014, the CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course was held at Roswell Park Cancer Institute, in Buffalo, NY, USA, organized by Naomi Nakagata (CARD-Kumamoto University, Japan), Aimee Stablewski (Roswell Park Cancer Institute, Buffalo, NY, USA) and Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA). This was the second CARD course organized overseas, outside Asia, after the first course organized in Madrid in October 2013. This new practical course in North America was co-sponsored by the International Society for Transgenic Technologies (ISTT). What follows is a brief course report prepared by Aimee Stablewski and Jan Parker-Thornburg, who deserve to be praised, along with Naomi Nakagata and his CARD team, for another most successful cryopreservation course. This course report can also be downloaded from here.

CARD-­RPCI Spermand Embryo CryopreservationWorkshop
September 15-­19, 2014
Roswell Park Cancer Institute, Buffalo NY, USA


The CARD-RPCI Sperm and Embryo Cryopreservation Workshop was recently held in Buffalo NY USA on the campus of Roswell Park Cancer Institute. Eighteen trainees, fifteen instructors and lecturers, and numerous vendors were hosted by Naomi Nakagata (Kumamoto University), Aimee Stablewski (Roswell Park Cancer Institute) and Jan Parker-Thornburg (MD Anderson Cancer Center) from September 15-19, 2014. Drs. Naomi Nakagata and Toru Takeo brought their team from CARD to assist the trainees in learning the latest techniques in embryo vitrification, sperm cryopreservation, in vitro fertilization, and the new method of vitrifying oocytes. In addition, Jorge Sztein instructed the trainees in methods of ovary freezing and subsequent implant after thaw; Lluis Montoliu provided lectures in current methods of embryo cryopreservation and CRISPR/Cas9 methods of generating genetically engineered mice, and Barbara Stone provided training in using the NSET method of embryo implant.

This was the first time that the CARD team provided training in North America, and the team (supported by Kumamoto University and Kyudo Co., LTD.) delivered a truly memorable experience for the trainees. Hands-on sessions were intense and intended to provide trainees with the knowledge and experience of performing the exacting CARD techniques. The eighteen trainees were composed of fourteen ISTT members and four non-members. Of these, three were from Europe, one from Australia, one from New Zealand, and the remainder from all parts of North America.

Hands on training included isolating unfertilized mouse oocytes, isolating and cold storage/shipping of mouse cauda epididymis, freezing/thawing mouse sperm and IVF using CARD frozen sperm and legacy sperm, fresh mouse sperm and IVF, cold stored sperm and IVF, freezing/thawing 2-cell IVF-derived mouse embryos, vitrification of mouse oocytes and embryos, IVF of vitrified mouse oocytes, ovary transplantation/ovary freezing, and embryo transfer techniques in mice (oviduct, uterus via NSET).

Didactic lectures were given on the topics of historic and scientific perspectives of embryo and sperm cryopreservation (by Jorge Sztein), comparing current embryo and sperm cryopreservation methods (by Lluis Montoliu), vitrification of oocytes and their use for IVF (by Naomi Nakagata), new US guidelines for the use of animals in research/IACUC (by Sandra Sexton), CRISPR/Cas9 and gene editing endonucleases (by Lluis Montoliu), development of database for managing mouse banking system (by Shuuji Tsuchiyama), shipping mice, frozen or refrigerated embryos/sperm across the world (by Toru Takeo) and freezing and transplantation of ovaries (by Jorge Sztein).

On Wednesday of the workshop, all of the participants were treated to a trip to Niagara Falls followed by an exceptional gala dinner (arranged by Aimee Stablewski) at a local winery. This short respite enabled the participants to replenish their energies for subsequent long days of IVF, vitrifications and surgery.

In all, it proved to be an exceptional workshop, with all participants achieving exceptional results in most, if not all, of the practicals. In fact, the depth of appreciation for being taught these methods became clear both during the closing session, where all instructors, vendors, and participants thanked Dr. Nakagata with a standing ovation, and immediately afterward, where many compliments were given. Perhaps, one of the participants said it best in an e-mail immediately following the course:

a big thank you to Prof. Nakagata, Dr. Toru Takeo and the entire CARD team. The way they have organised is unbelievable. As we all know in biological practical experiments, how much ever we take care, they never go according to plan and there is always a blooper. I must confess, I had my doubts that we would ever stick to the time table. I am glad that I was completely proven wrong. They have given attention to every bit of details and must have put lot of rehearsals behind this. They were fantastic. They answered all the questions and made sure everyone understood plus followed it up with the practicals. The Entire Card team was simply amazing and no other way to express my gratitude than simply saying “I bow to the entire CARD team”.”
Prasanna Kallingappa
University of Aukland
New Zealand

Aimee Stablewski and Jan Parker-Thornburg, as hosts, would like to acknowledge not only our lecturers, but also the CARD team who assisted the students, including Shuuji Tsuchiyama, Kiyoko Fukumoto, Yukie Haruguchi, Tomoko Kondo, Yumi Takeshita, Yuko Nakamuta, Tomoko Umeno, (all from Kumamoto University and Kyudo Co. LTD); and the CARD “adoptees”—Kristy (Kinchen) Williams (University of Florida) and Amar Dasari (Taconic). In addition, technical assistance was ably provided by Dawn Barnas (ISTT), Karstin Webber, Sandra Sexton and Leslie Curtin (all RPCI), who treated over 600 mice with hormones!

The workshop was extremely fortunate to have extremely generous vendor support, including our platinum sponsors: Charles River Laboratories (who supplied all of the mice used in the course), Leica Microsystems (who provided all of the microscopes used for the course as well as onsite support by Louise Bertrand), and Kyudo Co., LTD who provided support for the CARD instructors as well as onsite support by Nobuyuki Mikoda; our gold sponsors: the ISTT (who sponsored Lluis Montoliu’s participation and contributed to the gala dinner), Taconic (who provided instructional support from John Couse and Amar Dasari), and Regeneron (who sponsored a lecture by Lluis Montoliu); our silver sponsors: Hamilton Thorne (who provided instruction in sperm analysis and laser-assisted IVF by Nancy Mutch), IDEXX Bioresearch for course support, Paratechs for sponsoring Dr. Barbara Stone’s participation, Cell Preservation Solutions who provided course support and Lifor media for cold-storage, CosmoBio who provided course support, Transposagen who provided course support, and our bronze sponsors: Millipore EMD and mofa for equipment, Tokai-Hit for equipment, Eppendorf (Mike Bady) for course support and equipment, and VWR and Sarstedt for course support.

Finally, we thank our participants for their hard work and dedication to bring the newest transgenic technologies back to their institutions.

Aimee Stablewski, Roswell Park Cancer Institute, Buffalo, NY, USA
Jan Parker-Thornburg, MD Anderson Cancer Center, Houston, TX, USA

The ISTT is moving from Europe to America

Sunday, August 31st, 2014
The ISTT is moving from Europe to America

The ISTT is moving from Europe to America

The International Society for Transgenic Technologies (ISTT) will soon move its headquarters from Europe to America, from Spain (Europe Union) to the State of New York (United States of America). After the 12th Transgenic Technology (TT) meeting in Edinburgh, TT2014, the ISTT Society will be legally represented by a new non-profit entity: ISTT, Inc., registered in the State of New York (USA) in May 2013, after the corresponding approval was obtained from ISTT members at the Extraordinary General Assembly held at the TT2013 meeting, in Guangzhou, China, in February 2013. This moving process was essentially undertaken to allow any member to run for any position at the Board and to reduce tax running costs. The process is meant to be smooth and transparent to all members which will be kindly requested to join the new entity, to approve the new bylaws and to approve the new Board. Everything else will remain the same and the ISTT members will continue enjoying the same benefits from being part of the ISTT Society. The ISTT thank all of its members for their support and collaboration.

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