The TT2013 meeting report, written by Douglas Strathdee (Beatson Institute for Cancer Research, Glasgow, Scotland, UK) and C. Bruce A. Whitelaw (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) has just been published, online, at the Transgenic Research journal web site. This review, entitled ‘TT2013 meeting report: the Transgenic Technology meeting visits Asia for the first time‘ nicely summarizes the talks and activities held during the recent 11th Transgenic Technology meeting, held in Guangzhou (China), on February 25-27, 2013, along with the subsequent hands-on workshop that was organized, on February 28-March 2, 2013. Douglas and Bruce, together with Peter Hohenstein (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) are the Organizers of the next 12th Transgenic Technology meeting, TT2014, which will be held in Edinburgh (Scotland, UK) on October 6-8, 2014.
Archive for the ‘web site’ Category
Mouse sperm cryopreservation: satellite workshop at the 2013 FELASA-SECAL Congress in Barcelona, Spain, 10 June 2013Tuesday, March 26th, 2013
The International Society for Transgenic Technologies (ISTT) is pleased to announce the approved co-sponsorship of the Mouse Sperm Cryopreservation satellite workshop, organized by Jorge Sztein (NIH, Rockville, MD, USA) and Jesús Martínez Palacio (CIEMAT, Madrid, Spain), both ISTT members, in Barcelona (Spain) on 10 June 2013, within the activities associated to the 2013 FELASA-SECAL Congress in Barcelona, Spain, 10-13 June 2013. The main objective of this workshop is to acquaint students on reliable methods of mouse sperm cryopreservation, Jax and Nakagata, without requiring appliances or large investments to establishing a program at their centers. This course involves manipulation of animals and LN2.
This half-day satellite workshop will be held on 10 June 2013 from 09.00 to 12.00 h. at the Faculty of Medicine, University of Barcelona, Experimental Animal Unit. Satellite workshop registration fee: €120. Seats are limited to 24 participants. ISTT members, including those already registered to attend this workshop, are entitled to 25% discount. Interested participants should contact: email@example.com
EMBO Practical Course – Developmental neurobiology: From worms to mammals, 30 June to 13 July 2013 – London, UKMonday, March 25th, 2013
The International Society for Transgenic Technologies (ISTT) will be pleased to support the 2013 Edition of the EMBO Practical Course on Developmental Neurobiology: From Worms to Mammals, which will be held at the MRC Centre for Developmental Neurobiology, King’s College London, UK, on 30 June to 13 July 2013, organized by Robert Hindges (ISTT member) and other colleagues. The ISTT will be promoted among lecturers, instructors and participants at this course and, at the same time, several ISTT gadgets will be distributed among participants too. According to the Course web site, the main objetives of this 2013 EMBO course are:
- Learn the state of the art techniques used in modern developmental neuroscience
- Acquire a knowledge of brain development in 6 different species (C. elegans, Drosophila, zebrafish, Xenopus, chick and mouse)
- Interact with international leading neuroscientists
“Yale scientist Francis Hugh (Frank) Ruddle, a pioneer in genetic engineering and the study of developmental genetics, died March 10 in New Haven. He was 83 years old“. This is how a text in memoriam of Frank Ruddle, who passed away last Sunday, begins, at the Yale University web site. Frank Ruddle was Professor Emeritus at the Department of Molecular and Developmental, Yale University. In collaboration with Jon W. Gordon, from the Mount Sinai School of Medicine in New York, at the time one of his post-docs, Frank Ruddle devised and succeeded in creating the first transgenic mice, the first animals genetically modified after microinjecting a plasmid DNA into the pronuclei of fertilized mouse eggs. These seminal papers were published in 1980 and 1981. In order to remember Frank Ruddle’s pioneer contributions to the field of transgenic technologies, to highlight the relevance of their findings among our youngest colleagues, and to adequately assess, in perspective, their fantastic achievements, made more than 30 years ago (or only 30 years ago, depending how you would like to see the case) I am citing here the full abstracts, as they appear published in their respective journals, PNAS (in 1980) and Science (in 1981).
Genetic transformation of mouse embryos by microinjection of purified DNA.
Gordon JW, Scangos GA, Plotkin DJ, Barbosa JA, Ruddle FH.
Proc Natl Acad Sci U S A. 1980 Dec;77(12):7380-4.
ABSTRACT: “A recombinant plasmid composed of segments of herpes simplex virus and simian virus 40 viral DNA inserted into the bacterial plasmid pBR322 was microinjected into pronuclei of fertilized mouse oocytes. The embryos were implanted in the oviducts of pseudopregnant females and allowed to develop to term. DNA from newborn mice was evaluated by the Southern blotting technique for the presence of DNA homologous to the injected plasmid. Two of 78 mice in one series of injections showed clear homology, though the injected sequences had been rearranged. Band intensities from the two positive mice were consistent with the presence of donor DNA in most or all of the cells of the newborns. These results demonstrate that genes can be introduced into the mouse genome by direct insertion into the nuclei of early embryos. This technique affords the opportunity to study problems of gene regulation and cell differentiation in a mammalian system by application of recombinant DNA technology.”
Integration and stable germ line transmission of genes injected into mouse pronuclei.
Gordon JW, Ruddle FH.
Science. 1981 Dec 11;214(4526):1244-6.
ABSTRACT: “Genetic material has been successfully transferred into the genomes of newborn mice by injection of that material into pronuclei of fertilized eggs. Initial results indicated two patterns of processing the injected DNA: one in which the material was not integrated into the host genome, and another in which the injected genes became associated with high molecular weight DNA. These patterns are maintained through further development to adulthood. The evidence presented indicates the covalent association of injected DNA with host sequences, and transmission of such linked sequences in a Mendelian distribution to two succeeding generations of progeny.”
In summary, they injected some heterologous DNA into the pronuclei of fertilized mouse eggs. This DNA was eventually covalently associated with the host DNA (integrated) and was also successfully transmitted to the progeny of the resulting genetically-modified mice (transgenic), therefore it was inherited as a new DNA piece, a new genetic trait, thereby creating a new mouse strain, a new animal model, a trangenic mouse. Isn’t that splendid and beautifully simple? Indeed, but someone had to envisage first the experiment, someone had to carry out the injections successfully. Someone was first in demonstating this was actually possible. This was Frank Ruddle.
On behalf of the International Society for Transgenic Technologies (ISTT) I want to express my most sincere condolences to his family, colleagues and friends. This is a great loss for the transgenic community.
The recent and most successful 11th Transgenic Technology (TT2013) meeting, held in Guagnzhou (China) on 25-27 February 2013 and organized by Prof. Ming Zhao (Southern Medical University)l, on behalf of the International Society for Transgenic Technologies (ISTT), has been reported, commented and covered by pictures, videos and TV- interviews at the most popular and visited Chinese science-oriented web portal biodiscover.com. Footage includes TV-interviews to Prof. Ming Zhao, Chair of the TT2013 meeting; Prof. Xiang Gao, Director of the Model Animal Research Center of Nanjing University, Nanjing, China; Prof. Zhu-gang Wang, Director of the Shanghai Research Center for Model Organisms, Shanghai, PR China; and Dr. Lluis Montoliu, President of the ISTT, among other.
Immediately after the Chinese Spring festival, the Chinese New Year’s celebrations, the ISTT family was gently bitten by the Chinese snake. The 12th Transgenic Technology meeting, TT2013, held in Guangzhou, the capital of the province of Guandong, inaugurated the year of the snake, in the Chinese calendar. Ancient Chinese wisdom says a snake in the house is a good omen because it means that your family will not starve. People born in the Year of the Snake are keen and cunning, quite intelligent and wise. They are great mediators and good at doing business. Therefore, all the popular Chinese signs already indicated we would be enjoying a very successful and lucky TT2013 meeting. And, I’m most glad to say this was indeed the case!. The ISTT family and newcomers we all enjoyed an excellent conference where all invited speakers contributed extensively to this success, sharing their latest and most exciting results in the field of animal transgenesis and triggering interesting discussions. The quality of the talks was excellent and, noteworthy it was impressive to learn and discover how advanced and innovative to different aspects of the genetic modification of animals our Chinese colleagues are and have been progressing, thanks to a clear support from their Chinese Government and institutions. The influential Chinese science was represented at this TT2013 meeting by several top scientists in their respective field, using rodents, livestock or fish as experimental animal models, and all working in China, including: Qi Zhou, Jinsong Li, Xiang Gao, Bo Zhang, Liangzue Lai, Depei Liu, Guo-Liang Xu, Ning Li and Zhu-Gang Wang.
Ming Zhao, the Chair of the TT2013 meeting, from the host institution, the Southern Medical University of Guangzhou, worked very hard, leading a large group of local collaborators that brought the TT2013 conference to a success. The TT2013 meeting kicked off with a pre-meeting dinner, at a cruise upon the Pearl River, another majestic snake with multiple branches that crosses the city of Guangzhou at many locations. As soon as the darkness covered the city we discovered numerous impressive buildings and bridges, nicely illuminated with colorful neons, dominated by the Canton Tower, an outstanding communication tower more than 600 meters height.
The TT2013 meeting took place at the Baiyun International Convention Center (BICC) in Guangzhou, a gigantic conference center holding hotels, restaurants, seminar rooms and anything a meeting venue would require. We only occupied a small fraction of one of the five dedicated buildings of this enormous conference complex, and everything we needed, the seminar room, the exhibitor’s hall, the poster boards and the eating and drinking places were tightly and nicely grouped into one single location. Furthermore, most of TT2013 delegates were also lodged in one of the two large hotels of the BICC complex. Therefore we had everything we required handy and concentrated.
About 350 delegates gathered for the TT2013 meeting in Guangzhou, including representatives from 31 exhibiting companies that sponsored the conference and, hence, decisively contributed to its success. All sponsors must be praised for their most generous support. The conference included talks from 33 invited speakers, coming from many countries around the world, from Europe, America, Australia and Asia, thus highlighting the well-known international soul of the Society, also regularly reflected at the TT meetings. TT2013 participants were coming from as many as 27 different countries. Such phenomenal enterprise could not be managed without the devoted hard work of Ming Zhao’s large group of collaborators from the Southern Medical University, led by W. Chen, Xianyan Liu, Bibo Liang, Xiangguang Wu and all the rest of young and helpful students appointed by the chair of this conference. Their committed work must be acknowledged and commended.
In Guangzhou, at the TT2013 meeting, the 9th ISTT Prize for outstanding contributions to transgenic technologies, generously sponsored by genOway, was awarded to Allan Bradley, Director Emeritus of the Wellcome Trust Sanger Institute (WTSI), in Hinxton, UK, and leader of the Mouse Genomics Team at WTSI. Upon receiving the ISTT Prize, he delivered a great inspirational talk about his personal journey over three decades (1980-2013) on embryonic stem cells technologies he had the privilege to witness and be part of it from the first row. In awarding this ISTT prize to Allan Bradley, the ISTT Prize committee acknowledged his many fundamental contributions to the science and technology of manipulating the mouse genome. His pioneering mouse embryonic stem (ES) cell work in the 1980′s, demonstrating germ-line transmission and the great potential of ES cells to generate mice carrying mutations in endogenous genes, established milestones in a field that saw the award of the 2007 Nobel Prize in Physiology or Medicine to Capecchi, Evans, and Smithies. Later, he generated a number of broadly relevant knockout mouse models that are still used regularly today. His subsequent research has developed new methods for the genetic analysis and genetic modification of mice. These developments have been instrumental for advancing mouse genetics studies and the use of mice to understand the human genome. Furthermore, his strong vision and leadership at the Wellcome Trust Sanger Institute, which he directed from 2000-2010, was key to creating the international consortia whose aim was to systematically disrupt every gene in the mouse genome, resulting in a massive impact on the field of transgenic technologies. In Guangzhou, the sponsor of this ISTT Prize, genOway, was represented by Yacine Cherifi.
Another important award that has rapidly gained prestige and recognition among the youngest researchers and technologist in our field is the ISTT Young Investigator Award. The ISTT Young Investigator Award recognizes outstanding achievements by a young scientist who will keep the field of transgenic technologies vibrant with new ideas and who has recently received his or her advanced professional degree. The ISTT Young Investigator Award is generously sponsored by inGenious Targeting Laboratory (iTL). At the TT2013 meeting in Guangzhou, the second edition of this ISTT Young Investigator Award was received by Toru Takeo, from the Center for Animal Resources and Development, Kumamoto University, Japan. The Award committee considered that Toru Takeo’s work, in Naomi Nakagata’s laboratory, represented a major improvement of mouse sperm cyropreservation and IVF techniques, thereby greatly facilitating the archiving and sharing of many mouse models produced by the transgenic community. Toru Takeo summarized his recent achievements in mouse sperm cryopreservation and IVF with a very interesting talk where he highlighted the value and uniqueness of receiving a combined training in Pharmaceutical Sciences and Reproductive Biology, leading to his successful use of several drugs and compounds that have boosted sperm cryopreservation efficiencies in mice. In Guangzhou, the sponsor of this ISTT Young Investigator Award, inGenuious Targeting laboratory, was represented by Ailan Lu.
At the end of the TT2013 meeting, as it has become a tradition in the closing ceremonies of TT meetings, since the TT2015 meeting in Barcelona, it was the time to reveal and present the next venue for the following conference, the 12th Transgenic Technology meeting, TT2014, which will be held in Edinburgh, Scotland, UK. Bruce Whitelaw (Roslin Institute, University of Edinburgh) and Douglas Strathdee (Beatson Institute, Glasgow) represented the local Organizing Committee (the third Organizer, Peter Hohenstein, could not attend the meeting in China). Douglas Strathdee, Chair of the TT2014 conference, introduced their vision and aims for the TT2014 meeting in Edinburgh, depicting a combination of well-consolidated sessions, already classical at TT meetings, with some interesting innovative challenges, including an increased number of short oral presentations, Poster teasers and a practical workshop on zebrafish transgenic technologies. Additional information for this TT2014 meeting will be regularly available from its corresponding web site: www.tt2014.org. The Organizers have already activated an official TT2014 meeting email address to receive suggestions, inquiries, comments and any request of information related to this conference: firstname.lastname@example.org.
Finally, last but not least, once the TT2013 meeting was finished, immediately next, the relay was passed to Liangping Li and Wenhao Xu, who, together with Jing An and Ming Zhao, organized a practical hands-on transgenic workshop, hence fulfilling one of the most important missions of our Society, that of teaching, forming, educating scientists and technologies with the latest methods in the generation and analysis of genetically-modified animal. This practical course was chaired by Wenhao Xu and hosted by Liangping Li, at the newly refurbished and excellently equipped Disease Model Animal Center of the Sun Yat-Sen University in Guangzhou, one the biggest and most prestigious universities in China. 30 participants attended this 2013 Transgenic Workshop and were taught by a team of generous instructors from various countries in the world, including ISTT members and delegates from the sponsoring companies. A variety of microinjection techniques were discussed and performed by the participants, including DNA embryo pronuclear injection, laser-assisted injection of ES cells, piezo-assisted ICSI, non-surgical embryo transfer, colony management and assisted reproduction techniques, among other useful methods in the daily work of a modern transgenic facility.
In summary, the TT2013 meeting and 2013 Transgenic Workshop in Guangzhou, China, have been again two most successful and memorable events. On behalf of the International Society for Transgenic Technologies (ISTT), I can only express my deepest appreciation to Ming Zhao, Liangping Li, Wenhao Xu, Jing An and the rest of members of the local organizing and advisory committees for having put together such a wonderful conference and practical course, thereby maintaining and further expanding the highest standards and quality of the TT meetings series. Alison Cameron, our ISTT administrative assistant, deserves here to be greatly acknowledged for her instrumental collaboration during the preparation of this TT2013 meeting and workshop and for her helpful contribution to the success of this first visit of the ISTT family to Asia. We can conclude by stating that, yes, the ISTT family was gently bitten by the Chinese snake, and this resulted in a rewarding and unforgettable experience.
The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course that will be held at the National Centre for Biotechnology (CNB-CSIC), in Madrid, CSIC, during one week, on 7-11 October 2013, organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD) and Lluis Montoliu (CNB-CSIC, Madrid, Spain, Coordinator of the Spanish EMMA node).
This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to email@example.com by 31 May 2013.
The maximum number of participants attending this course will be 20, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted until 31 May 2013. The review and selection of participants will be done by the Organizers from 1 to 15 June 2013. Registrations and payments for selected participants will be accepted from June 16, 2013 to August 31, 2013. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by 31 August 2013 will be readily substituted by the first available person from this waiting list, starting on 1 September 2013.
The course registration fee is 800 Euros (with a reduced fee of 750 Euros for ISTT Members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a convenient nearby hotel, close to CNB Campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.
COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines. At the CNB-CSIC in Madrid, hosting the Spanish EMMA node, these new CARD cryopreservation methods have been successfully implemented and, moreover, a fruitful collaboration has been established with the laboratory of Prof. Naomi Nakagata, eventually resulting in the signature of an institutional cooperation agreement between the CSIC and the University of Kumamoto, under the framework of which this cryopreservation course is organized. The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 20.
Hands-on topics that will be covered during this cryopreservation course
- making pipettes and practising embryo handling
- isolating unfertilized mouse oocytes
- isolating and cold storage/shipping of mouse cauda epididymis
- freezing/thawing mouse sperm and IVF
- fresh mouse sperm and IVF
- freezing/thawing 2-cell IVF-derived mouse embryos
- vitrification of mouse oocytes and embryos
- embryo transfer techniques in mice
- vasectomy of male mice
- demonstration of ICSI
Additional lectures on the following topics
- new EU Directive on the protection of animals for experimentation
- new US Guidelines for the use of animals in research
- epigenetic effects of in vitro culture on mouse embryos
- databases for handling information in cryopreservation facilities
- shipping mice, refrigerated and frozen material
- safety issues handling liquid nitrogen in a cryopreservation facility
- EMMA and Infrafrontier-I3
- Naomi Nakagata (CARD-Kumamoto University, Japan)
- Toru Takeo (CARD-Kumamoto University, Japan)
- Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
- Tomoko Kondo (CARD-Kumamoto University, Japan)
- Yukie Haruguchi (CARD-Kumamoto University, Japan)
- Yumi Takeshita (CARD-Kumamoto University, Japan)
- Yuko Nakamuta (CARD-Kumamoto University, Japan)
- Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
- Raul Fernández (INIA, Madrid, Spain)
- Lluis Montoliu (CNB-CSIC, Madrid, Spain)
- Julia Fernández (CNB-CSIC, Madrid, Spain)
- María Jesús del Hierro (CNB-CSIC, Madrid, Spain)
- Marta Castrillo (CNB-CSIC, Madrid, Spain)
- Isabel Martín-Dorado (CNB-CSIC, Madrid, Spain)
- Kristy Kinchen (Mouse Biology Program, UC Davis, CA, USA)
Additional lectures by
- Naomi Nakagata (CARD-Kumamoto University, Japan)
- Toru Takeo (CARD-Kumamoto University, Japan)
- Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
- Alfonso Gutiérrez-Adán (INIA, Madrid, Spain)
- Kent Lloyd (Mouse Biology Program, UC Davis, CA, USA)
- Belén Pintado (CNB-CSIC, Madrid, Spain)
- Jesús Martínez Palacio (CIEMAT, Madrid, Spain)
- Javier Guillén (AAALAC, Pamplona, Spain)
- Lluis Montoliu (CNB-CSIC, Madrid, Spain)
The International Society for Transgenic Technologies (ISTT) has decided, once again, to co-sponsor the IX Transgenic Animal Research Conference, hosted by the Department of Animal Science, UC Davis, and organized by ISTT Member Prof. James D. Murray. This conference will be held at the Granlibakken Conference Center, in Tahoe City, CA, USA, on 11-15 August 2013. This is a classical conference, most complementary to the TT meeting series, and specifically devoted to basic and applied projects, research and technical developments using non-rodent genetically modified animals. The ISTT is proud to have supported the two previous UC Davis Transgenic Conferences in Tahoe held in 2009 and 2011.
According to the conference web site: This is the ninth international meeting hosted by UC Davis to bring together representatives from the leading laboratories worldwide doing cutting edge work on transgenic research in non-murine animals, including livestock, fish and poultry species. The previous meetings were each attended by up to 160 participants from 12 to16 different countries throughout the globe. Each conference, in addition to reviews and papers on transgenic animals, included presentations covering technical developments in areas such as nuclear transfer-based cloning, cell transformation, vector design, and nuclease-directed gene insertion that affect the production of transgenic animals. Oral presentations are by invitation, with participants encouraged to contribute poster presentations. The upcoming conference will again focus on state-of-the-art science in the field of transgenic research. Presentations will address cutting-edge methodology, technical improvements, and current progress towards producing transgenic animals for biomedical and agricultural applications. The intent of these meetings is to bring together scientists to discuss progress, problems, and potential application of transgenic technology for animal applications. The meeting will consist of invited presentations and submitted posters. Two afternoons from noon to 4 p.m. and one evening will be free to allow for small group interactions and to take advantage of the great natural beauty and recreational activities in the Lake Tahoe area.
Already appointed and confirmed Speakers include:
- Matt Wheeler (Illinois) – opening talk
- Bruce Whitelaw (Edinburgh) – closing talk , ISTT Member
- Scott Fahrenkrug (Minnesota) – TALENS in livestock
- Emmanuelle Charpentier (Germany/Sweden) – CRISPR RNA-programming technology
- Caitlin Cooper (Davis) – feeding hLZ and hLF milk to pigs
- Hongbin He (China) – FMDV resistance
- Angelika Schnieke (Germany) – cancer models in pigs
- Chuck Long (A&M) lentiviral production of livestock
- Irina Polejaeva (Utah) – tg goat cardiovascular models
- Goetz Laible or Stefan Wagner (NZ) – KO of beta-lactoglobulin and transduction of the mammary gland
- Rob Etches (Crystal Biosciences) – heavy chain KO chickens
- Yonglun Luo (Alun) (Denmark) – adeno-assoc HR + talens / BCRA-1
- Mike McGrew (Roslin) growing and modifying avian primordial germ cells, including transposons
- Tim Doran (Aust) Virus disease resistant transgenic poultry and fish
- Derek Nimmo (UK) – TG mosquito for control of dengue fever
The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the 7th Workshop on Innovative Mouse Models (IMM2013), which will be held at the Leiden University Medical Center (LUMC), in Leiden, The Netherlands, on 13-14 June 2013. This will be the third consecutive edition of this meeting series that is granted with the co-sponsorship of the ISTT, after the IMM2011 and the IMM2009 editions, due to its interest and relevance for the community of researchers using genetically-modified mice. The IMM2013 workshop is organized by: Jos Jonkers (NKI-AVL, Amsterdam, The Netherlands), Paul Krimpenfort (NKI-AVL, Amsterdam, The Netherlands), Werner Mueller (University of Manchester, Manchester, United Kingdom), Hein te Riele (NKI-AVL, Amsterdam, The Netherlands), Els Robanus-Maandag (LUMC, Leiden, The Netherlands), Marian van Roon (VU, Amsterdam, The Netherlands and ISTT Member) and Sjef Verbeek (LUMC, Leiden, The Netherlands and ISTT Member).
According to the IMM2013 workshop web page: The primary goal of this two-day workshop is to bring together a diverse group of scientists interested in advanced genome alteration approaches in the mouse, including key developers of emerging technologies as well as researchers who wish to apply and assess these new approaches. The IMM2013 workshop will encourage an in-depth and unvarnished discussion of these technologies and novel developments. This 2-day workshop will have a mixture of invited speakers and selected presentations. Keynote lectures will be given by:
- Anton Wutz (UK) haploid ESC
- Haoyi Wang (US) iPS / TALENs
- Bill Skarnes (UK) high throughput TALENs
- Ben Davis (UK) docking site/ new recombinases
- Yann Herault (France) Cre transgenic mice
- Kevin Brindle (UK) MRI
- Mathijs Verhagen (NL) large scale phenotyping
- B Kappes (US) TALENs
- Zoltan Ivics (Germany) Transposons
In about two weeks, and starting on February 25, the International Society for Transgenic Technologies (ISTT) will be holding the 11th Transgenic Technology (TT2013) meeting, at the Baiyun International Convention Center, in Guangzhou/Canton, Guandong, China, followed by the hands-on workshop on basic microinjection techniques. The TT2013 meeting is the most important event promoted from the International Society for Transgenic Technologies (ISTT). The TT2013 meeting is organized by Prof. Ming Zhao (China) and the subsequent hands-on workshop by Dr. Wenhao Xu (USA), Prof. Liangping Li (China) and Prof. Ming Zhao.
The TT2013 meeting web page within the ISTT web site is available from: www.transtechsociety.org/tt2013/
I would like to thank all Organizer and Advisory Committees, all Exhibitors and Sponsors for contributing with their work and support to the success of the TT2013 meeting.
See you soon in China!