Ana Isabel Nieto (Granada, Spain) is the winner of a copy of the ISTT Manual:Advanced Protocols for Animal Transgenesis (edited by Shirley Pease and Thomas Saunders, published by Springer, 2011) from bioterios.com, the most popular South-American web portal for Laboratory Animal Science, in Spanish, organized and led by the ISTT member Juan Manuel Baamonde (CECs, Valdivia, Chile). The International Society for Trangenic Technologies (ISTT) and bioterios.com are mutually supporting and promoting their activities. The web portal bioterios.com has been referred previously from this ISTT blog, and also the launching of their new web layout, earlier this year. Likewise, the ISTT and the TT2014 meeting have also been promoted from bioterios.com.
Archive for the ‘web site’ Category
Today, the 12th Transgenic Technology (TT2014) meeting web site has been launched. And meeting registration is already open!. The TT2014 meeting is organized by ISTT members Douglas Strathdee-chair, Peter Hohenstein and Bruce Whitelaw and will be held at The Assembly Rooms, in Edinburgh, Scotland, UK, on 6-8 October 2014. Immediately following the TT2014 meeting, on October 9-10, 2014, there will be a hands-on practical workshop called ‘An Introduction to Zebrafish Transgenesis‘ which will focus on Zebrafish. Further details about this practical workshop will be announced at the TT2014 meeting web site.
The meeting is hosted by three world-class Scottish research institutes and the University of Edinburgh: the Roslin Institute; the Institute of Genetics and Molecular Medicine and the Beatson Institute for Cancer Research. All three Institutes are world-renowned for producing top quality science at the forefront of biomedical research. The TT meeting visits the UK for the first time following the previous TT meetings in Guangzhou, China (TT2013); Florida, USA (TT2011); Berlin, Germany (TT2010); Toronto, Canada (TT2008); Brisbane, Australia (TT2007) and Barcelona, Spain (TT2005). This will be the 12th meeting in the series, originally pioneered by Johannes Wilbertz (Karolinska Institute, Stockholm, Sweden) in 1999. Since the foundation of the ISTT in 2006, the TT meetings have been the main event sponsored by the Society.
The following speakers have confirmed their participation at the TT2014 meeting:
- David Adams, Wellcome Trust Sanger Institute, Hinxton, Cambridge UK
- Ignacio Anegon, Center for Research in Transplantation and Immunology, Nantes, France
- Stephen Ekker, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
- Kat Hadjatonakis, Developmental Biology Program, Sloan-Kettering Institute, New York, USA
- Coenraad Hendriksen, Institute for Translational Vaccinology, Bilthoven, The Netherlands
- Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Nine Cambridge Center Cambridge, USA
- Jos Jonkers, Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
- Keith Joung, Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA
- Alex Joyner, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
- Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics, Shizuoka, Japan
- Jim Murray, Department of Animal Science and Department of Population Health and Reproduction, University of California, Davis, California, USA
- Stephen Murray, The Jackson Laboratory, Bar Harbor, Maine, USA
- Lluis Montoliu, ISTT President, Organising Committee, National Center of Biotechnology (CNB), CSIC, Madrid, Spain
- Vasilis Ntziachristos, Technische Universität Mu?nchen, Munich, Germany
- Pawel Pelczar, Institute of Laboratory Animal Science, Zürich, Switzerland
- Janet Rossant, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
- Angelika Schnieke, Livestock Biotechnology, WZW Center of Life Science, Freising-Weihenstephan, Germany
- Kai Schönig, Central Institute of Mental Health, Heidelberg University, Mannheim, Germany
- Austin Smith, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK
- Sara Wells, MRC Harwell, Oxfordshire, UK
- Jacqui White, Wellcome Trust Sanger Institute, Hinxton, Cambridge UK
At the TT2014 meeting, the ISTT will be awarding the 10th ISTT Prize for outstanding contributions to the field of transgenic technologies to Prof. Janet Rossant (The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada). The ISTT Prize is generously sponsored by genOway.
At the TT2014 meeting, the ISTT will be also awarding the 3rd ISTT Young Investigator Award, generously sponsored by inGenious Targeting Laboratory. The ISTT Young Investigator Award recognizes outstanding achievements by a young scientist who will keep the field of transgenic technologies vibrant with new ideas and who has recently received his or her advanced professional degree.
At the TT2014 meeting, and for the first time, the ISTT Best Poster Awards, traditionally awarded to the best posters presented at the corresponding TT meeting, will be generously sponsored by Charles River.
Accepted abstracts submitted for the TT2014 meeting, will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.
A minimum of six registration awards for ISTT members will be sponsored by the International Society for Transgenic Technologies. Applications should be sent, along with the registration document to email@example.com by June 30, 2014. Award decisions will be communicated by July 15, 2014 and awardees will receive a diploma at the TT2014 Meeting.
- Abstract submission deadline June 30, 2014
- Application for ISTT registration awards deadline June 30, 2014
- Awards to be communicated by July 15, 2014
- Early Bird registration fee deadline July 31, 2014
- Standard Rate registration fee from August 1, 2014
- Late & On-Site Rate registration fee from September 22, 2014
As it is stated in the TT2014 meeting home page: “Scotland prides itself on both its life science research and the warm welcome given to visitors and looks forward to hosting TT2014“. Therefore, on behalf of the ISTT and of the TT2014 Organising Committee we invite you all to attend to the TT2014 meeting.
See you all in Edinburgh!
We have uploaded videos from 25 of the 37 talks from selected invited speakers attending the past 11th Transgenic Technology meeting (TT2013), held in Guangzhou (China), on 25-27 February 2013, and organized by Ming Zhao and his co-worker Xiangguang Wu, from the Southern Medical University in Guangzhou. Ming Zhao and Xiangguang Wu kindly provided the original videos from those invited speakers that approved their distribution among ISTT members, from the members-only area of the ISTT web site. On behalf of the International Society for Transgenic Technologies (ISTT) I would like to thank both the speakers who approved the distribution of these video-talks and our colleagues from Guangzhou for technical help and for providing the original video files. The videos presented from TT2013 include the following talks:
The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)
From Haploid Stem Cells to Transgenic Mice
Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China
Generation of genetically modified mice by oocyte injection of androgenetic haploid embryonic stem cells
Center for Animal Resources and Development, Kumamoto University, Japan
Overview and Progress in sperm cryopreservation and IVF
10:30 – 11:15 Coffee Break, Posters and Exhibitors, 45 min break
Australian Phenomics Facility, The Australian National University, Canberra, Australia
The Australian Phenomics Network: Creating and Delivering New Research Tools and Resources
College of Life Sciences, Peking University, Beijing, PR China
TALEN Mediated Genome Modification in Zebrafish
Guangzhou Institutes of Biomedicine and Health, Chinese Academy of Sciences, Guangzhou, PR China
Generation of PPAR? mono-allelic knockout pigs via zinc-finger nucleases and nuclear transfer cloning
Max-Planck Inst. for Immunobiology and Epigenetics, Freiburg, Germany
Developing cutting edge technologies while still maintaining excellent basic services
Biomodels Austria & Inst. For Biotech. in Animal Production, Tulln, Austria
The business aspects of running a transgenic unit
National Center of Biotechnology, CSIC, Madrid, Spain; ISTT President
Introduction to the 9th ISTT Prize for outstanding contributions to transgenic technologies
Allan Bradley, 9th ISTT Prize
Wellcome Trust Sanger Institute, Hinxton, UK
Embryonic stem cell technology 1980-2013: A personal journey over three decades
MD Anderson CC, Smithville, TX, USA
The impact of genetic background in mouse and rat models: concerns and solutions
The Jackson Laboratory, Bar Harbor, ME, USA
Assisted Reproduction Strategies in Mice Short Oral Presentations selected among submitted abstracts:
Institut Pasteur, Paris, France
Frozen versus fresh embryos for ES cell microinjections: a comparative study with BALB/cN and C57BL/6N strains from seven transgenic facilities
Instituto de Neurociencias de Alicante, CSIC/UMH, Alicante, Spain
A New Methology for serial sperm collection in Mice
Kyle D. Lutes
Department of Computer and Information Technology-CIT Faculty, Purdue University, West Lafayette, IN, USA
Computer software application options for laboratory data management
Animal ethics and animal rights: lessons from history and implications for the future
Wellcome Trust Sanger Institute, Hinxton, UKBalancing High Throughput Production and Phenotyping with Animal Welfare, Care and Husbandry
Institute of Farm Animal Genetics, Friedrich-Loeffler-Institut, Mariensee, Germany
Precision genetic engineering in the pig genome and skin transplantation between members of a syngenic clone cohort carrying different vital reporter transposon
Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan
Gene expression changes induced by intracitoplasmic sperm injection
Oregon National Primate Res. Center, OHSU, Beaverton, OR, USA
Primate chimeras and ES cells
Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France
Overview of cre-mouse lines resources
Institute of Laboratory Animals, Kyoto University, Kyoto, Japan
National BioResource Project for the Rat in Japan
Shanghai Research Center for Model Organisms, Shanghai, PR China
Studies on the physiological functions of novel G-protein-coupled receptors in mice
Wellcome Trust Sanger Institute, Hinxton, UK
Rapid and Efficient Reprogramming of Mouse Somatic Cells to iPS Cells Using Six Factors.
Toru Takeo, 2nd ISTT Young Investigator Award
Division of Reproductive Engineering, Center for Animal Resources and Development-CARD, Kumamoto University, Japan
Efficient production of mouse embryos via chemically assisted in vitro fertilization using frozen- thawed sperm
All these videos from talks at the TT2013 video are available for ISTT members, within the members-only area.
Meeting report: IX Transgenic Animal Research Conference. Granlibakken Conference Center, Tahoe City, California, USA, 11-15 August 2013Tuesday, August 20th, 2013
The IX Transgenic Animal Research Conference, organized by ISTT member Jim Murray (UC Davis), was held last week at the Granlibakken Conference Center, Tahoe City, California, USA. The unique and beautiful location of this meeting series, by Lake Tahoe, in Northern California, surrounded by woods and mountains (and sporting chipmunks and bears), triggered its magic again and, hence, this ninth TARC was a rewarding success. The conference was attended by about 100 delegates from academia and industry, representing groups primarily interested in the generation, analysis or marketing of non-rodent transgenic animal models, as well as regulators and representatives from governmental agencies. This conference was co-sponsored by the ISTT.
The meeting started with a most passionate keynote address by Matt Wheeler (University of Illinois, USA) who reminded us about our responsibility and the mission we all have as biotechnologists to improve the efficiency of food production in cattle, pigs, and also poultry (as adequately reminded by Helen Sang [Roslin Institute, UK]) , using our unique genetic tools and techniques. Dr. Wheeler provided a number of striking figures to highlight the extraordinary need for food in the near future: “estimates have suggested that we will need to increase our current food production by 70% by 2050. This means that we will have to produce the total amount of food each year that has been consumed by mankind over the past 500 years”. He also expressed regret at how transgenic large animal programs were declining in the US, in part due to the lack of trust in a regulatory process that has been witholding the approval of some early transgenic animals. One major example of this is the ongoing saga of the fast growing AquAdvantage transgenic salmon, produced by AquaBounty, not yet approved, more than 20 years after being first generated. Finally, he openly referred to the unacceptable cost for the world, of not using the most advanced genetic engineering techniques to improve food production. He concluded that “hunger is a curable disease”.
Scott Fahrenkrug (Recombinetics Inc., USA) continued with a most interesting talk describing how the new gene editing tools (i.e. TALENs) can be applied for direct livestock genetics. Using illustrative examples in pigs and cattle he demonstrated the efficient introduction of single and multiple subtle genetic changes, often found as rare alleles in some breeds and difficult to introduce in the animal of choice by standard genetic breeding program, where the segregation of traits would require tens of thousands of animals and many generations. This first of several talks on genome editing tools was followed by that of Emmanuelle Charpentier (Helmholtz Center for Infection Research, Germany), one of the pioneers and discoverer of the CRISPR-Cas9 system in bacteria, and its application for the efficient gene edition in mammals. She suggested that new applications will come from the use of new variants of the RNA-guided Cas9 endonuclease.
The second session started with a talk by Daniel Carlson (Recombinetics Inc., USA), who gave technical details of the experiments described briefly by Scott Fahrenkrug, highlighting the factors that can influence success when attempting to precisely edit the genome of livestock species (pig and cattle) with TALENs. Next, Charlotte Brandt Sorensen (Aarhus University, Denmark) reported on the efficient genome engineering in pigs using both recombinant adeno-associated virus (rAAV) and TALENs in order to generate swine animal models of breast cancer and Type II diabetes. The session concluded with a technical lecture delivered by Colin Fox (Genentech, USA), on their approaches to systematically and efficiently genotype complex genetic alterations in transgenic animals affecting multiple alleles.
The third session was focused on the use of pigs for a variety of purposes. First, Kevin Wells (University of Missouri, USA), reported on their advances in a gene stacking project, where the use of phiC31 integrase and its corresponding target sites was evaluated, in parallel to standard homologous recombination approaches, for the efficient cointegration of multiple alleles at discrete genomic locations. The session was completed with talks from two German groups, where Nikolai Klymiuk (Ludwig-Maximilian University, Germany) and Angelika Schnieke (Technische Univ. Muenchen, Germany) shared their progress in xenotransplation and the modeling of cancer disease in pigs, respectively.
The fourth session, on the conference’s second day, started with a talk by Liangxue Lai (Guangzhou Institutes of Biomedicine and Health, China) reporting on their progress with a series of pig models of human degenerative diseases including Parkinson, Ataxia (ALS), Huntington and Alzheimer. Liangxue Lai had also participated as invited speaker at the TT2013 meeting in Guangzhou, held previously this year. Chuck Long (Texas A&M University, USA) presented work from his lab using lentiviral transgenes in cattle to knock-down the myostatin locus by RNA-interference. He also reported on a new model for muscle steatosis (marbling) in pigs. The session ended with a totally different animal system: chickens and avian primordial germ cells (PGCs), delivered by Mike McGrew (Roslin Institute, UK). Mike reported progress made in his lab to establish efficient conditions to culture chicken PGCs and his attempts to generate inducible knock-down of target genes using transposons and the TET-system.
The fifth session, with two talks, was entirely devoted to further evaluate risk assessment on the transgenic goat model producing lysozyme in milk, generated by Jim Murray and collaborators at UC Davis. First, Elizabeth Maga (UC Davis, USA) systematically analyzed whether there were any unintended effects associated with the mammary-specific expression of the lysozyme transgene in the host (lactating goats) and in a non-targeted organism (kid goats consuming the milk from transgenic goats). Even though they found some statistically significant differences among the many tests conducted, these were considered of no biological relevance, more due to time of expression and not due to the presence of the transgene. She concluded that there were no unintended effects as revealed in these analyses. Second, Caitlin Cooper (UC Davis, USA) shared her analysis on the effects of consumption of milk containing lactoferrin (from transgenic cows) and/or lysozyme (from transgenic goats) on the intestinal health in young pigs. Her studies concluded that lactoferrin and lysozyme exhibit both shared and unique mechanisms and highlighted the relevance of dosage in the positive effects observed in the intestinal villi architecture and the overall balance of several cells of the immunity system in the gut.
The sixth session presented two different but equally-interesting advances obtained by two agrobiotech companies. First, AgResearch’s researcher Goetz Laible (New Zealand) described their success in reducing the contents of beta-lactoglobulin (BLG) in ovine and cow milk, hence aiming to produce a less allergenic milk for eventual human consumption. They tested their strategy using RNA-interference in mice, with the help of some transgenic mice producing BLG in their milk. Finally, they generated a cow producing milk with reduced allergens. Next, Benjamin Schusser (Crystal Bioscience, Inc., USA) shared their advances towards producing therapeutic monoclonal antibodies against human proteins in chickens. In this regard, he documented the creation of the first chicken knockouts, for the IgL and IgH loci, by inserting the corresponding variable regions of human Ig loci.
The seventh session was also devoted to advances in chicken genetic engineering. Tim Doran (CSIRO, Australia) began with a description of an alternative way of genetically modifying chicken PGCs with transposon-type transgenes by direct in vivo transfection, thus avoiding the need to isolate, culture and reinsert these cells in host chicken embryos. This talk was followed by that of Mark Tizard (CSIRO, Australia), illustrating how the use of innovative RNA-interference approaches could be used for efficient trait control and disease resistance in poultry.
The conference’s last day started with three new large animal models for human diseases. First, Irina Polejaeva (Utah State University, USA) described her transgenic goat models that overexpress the profibrotic factor TGF-ß1 in cardiomyocytes, designed to study the relationship between cardiac fibrosis and atrial fibrillation. Next, Chris Rogers (Exemplar Genetics, USA) presented pig models for human hypercholesterolemia and atherosclerosis generated by disrupting the LDL receptor gene in the pig genome. The LDLR deficient pigs are currently being used to test new cholesterol-lowering drugs and to develop detection and treatment strategies for atherosclerosis. The session finished with a talk by Zhong Wang (University of Michigan, USA) and their new approaches to study heart development and regeneration in pigs.
The eighth session was devoted to the progress of animal products generated using biotechnology with regard to regulation and the expected path to market, once the product is investigated, validated and eventually approved by the relevant regulatory bodies. This process was described by Ronald Stotish (AquaBounty Technologies, USA), who shared the extremely long and as-yet unsuccessful attempt to obtain required FDA approval for marketing the AquAdvantage salmon. This fast-growing transgenic fish can grow to expected market size in half of the time required for non-transgenic salmon using standard aquaculture procedures. The apparent science-based regulatory process has been repeatedly interrupted by not only anti-technology groups but other groups with obvious political and economic interests conflicting with the marketing of these salmon. More than 20 years have passed since this transgenic salmon was first generated, and yet, after numerous scientific studies demonstrating that this product is as safe as non-transgenic salmon and after concluding that it does not pose a significant threat for the environment, the final approval by the FDA has not been issued. The seminar on the transgenic salmon issue was followed by a nice summary talk by Alison van Eennennaam (UC Davis, USA) where she presented how the regulation of genetically-modified animals is interpreted in different countries/continents, such as US, Europe or Australia, and the consequences these definitions have on the overall regulatory process aiming to obtain a permission to market a given transgenic animal or a product derived from them. Furthermore, she challenged the current regulatory scenario with the new gene editing tools (i.e. ZFNs, TALENs, or CRISPRs-Cas) where, in most cases, the genetic alterations leave no specific footprints and are undistinguishable from other similar genetic alleles that can be found in the nature, among the different breeds of a given species. Knowing in advance whether these precise genetic engineering processes will or will not be regulated through the current laws or whether they would require an adaption of current norms is of paramount importance for the progress of the animal biotechnology field.
The final session held two great but totally different talks. First, Derric Nimmo (Oxitec Inc., UK) described their elegant and innovative solution to efficiently down-regulate wild populations of mosquitoes (Aedes aegypti) This mosquito species survives by constantly feeding on human blood, and also serve as a vector to transmit serious diseases such as dengue or yellow fewer. He reported their approach using their RIDL strategy (Release of Insects with Dominant Lethality). The mechanism is based on a modified TET-off system where the tTA-VP16 activator is strongly expressed under several tet-op sequences unless the effector, Doxycycline (Dox), is provided in the diet. Hence, male transgenic mosquitoes can be raised in the laboratory, where the expression of the transgene is prevented with Dox, but, upon release in the wild, the lack of Dox triggers the expression of the transgene and the accumulation of the powerful transcriptional activators which cause irreversible damage to transgenic male mosquitoes, rending them sterile. Release of these sterile males and their subsequent mating with female populations is an efficient way to downsize wild mosquito populations. Approved open field tests have been already conducted in Cayman Islands, Malaysia and Brazil with success. The company is currently awaiting approval by the FDA and other equivalent agencies in order to apply their strategies in the US and other countries. This talk also illustrated the positive and rewarding effect accomplished by investing in informing people, affected populations, hospitals, governments, schools, etc… about this biotechnological approach to reduce disease-transmitting mosquitoes, which resulted in increased acceptance by the local populations. This community engagement approach appears to be the most promising and effective manner of gaining society’s acceptance for genetically-engineered animals and/or products.
The honor of the traditional concluding talk was given this time to Bruce Whitelaw (Roslin Institute, UK) with the challenge to envisage what the fourth decade would bring, after three decades of genetically engineered animals. After referring to the predicted needs for safe and more efficient food that this planet will need in the immediate future, Bruce divided the four decades as follows, identifying in each of them some major technological milestones: 1984-1993 (decade of the first transgenic animals produced by standard DNA pronuclear injection); 1994-2003 (decade of nuclear transfer, when Dolly was created and laid the foundation to generate many cloned and genetically-engineered mammals, using a technique currently referred as SCNT. At this point, Bruce kindly offered a tribute to the work done by Keith Campbell, instrumental in the creation of Dolly, who recently passed away); 2004-2013 (decade of a revolution in technologies including the use of lentivirus, transposons, SMGT, bird PGCs, ZFNs, TALENs and CRISPRs, and also, the decade of the first large animal models of human disease being effectively produced and tested). For the fourth decade, 2014-2023 Bruce speculated that the balance will re-equilibrate efforts and investments in both agricultural and biomedical sciences, after two decades where the genetic-engineering of animals was mostly dominated by projects and applications in biomedicine. He left us with the following thought: “The 4th decade of GE livestock is going to be good for those who work with this technology and for those – both man and animal – who benefit from it”.
All participants left home on August 15, after having enjoyed yet another fantastic conference put together by Jim Murray, who must be praised for his unrelenting commitment to this great meeting series, where the generation and application of non-rodent transgenic animals are discussed in depth, before, during and after the talks.
The next TARC meeting, the 10th Transgenic Animal Research Conference, will be held, at the same place, on August 9-13, 2015. We would encourage you to experience these meetings first hand, (and not through these meeting reports). Please make sure to book these dates on your agenda and not miss the next meeting by beautiful Lake Tahoe.
Lluis Montoliu & Jan Parker-Thornburg
BIOTERIOS.COM, the reference web portal on animal experimentation and animal welfare in Spanish in Latin America, created and maintained by Juan Manuel Baamonde (Manager of the animal facility at CECs, Valdivia, Chile, and ISTT Member) since 2007, has launched a new web page, a new layout, to show its very interesting and useful contents with a renewed and modern format. Among the new features that have been added, Juan Manuel must be praised for having included a web page translator tool (found at the top-right corner of all pages) which makes now possible to automatically translate the contents of any web page within BIOTERIOS.COM into another language of choice, to be selected among English, German, French or Portughese, hence further expanding the benefits of this wonderful site to all non-Spanish-speaking colleagues that could not read nor benefit from BIOTERIOS.COM before.
The site includes articles, interviews, reports on recent meetings and plenty of information on animal experimentation and animal welfare issues. Really worth visiting and exploring! (and now in English too!).
All the numerous tasks associated to the ISTT web server upgrade have been completed. The process took more time than anticipated but everything is now done and fixed. The ISTT web site and all its associated web resources are back to normal operation. Hope you will enjoy our new web server, with increased capacity. ISTT
Announcement: the ISTT web server will be upgraded on 5 July 2013, from 09:00 CET (Central Europe Time, Madrid, Spain) for an undetermined amount of time. During the upgrading process all web services and ISTT web-related resources (web page, email, lists, forum, etc…) will be closed down and interrupted. All web services will be resumed as soon as the upgrade has finished. We will communicate when the upgrading process has been completed. Thanks for your collaboration and kind understanding. ISTT webmaster.
The International Society for Transgenic Technologies (ISTT) will be participating and/or co-sponsoring numerous events during the month of June 2013. At first, on June 7, 2013, our ISTT colleagues from Nantes (France), Ignacio Anegon and Séverine Ménoret, experts in the generation of transgenic rats, will be holding their 2013 Nantes Transgenic meeting on “Technical advances in the generation of transgenic animals and in their applications“. Next, on June 10-13, 2013, in Barcelona (Spain), the 12th FELASA-SECAL congress will take place, where the ISTT will be participating in two ways. First, the ISTT will co-sponsor the satellite workshop on Mouse Sperm Cryopreservation, to be held within the 2013 FELASA meeting, on 10 June 2013, Barcelona, Spain, and organized by ISTT members Jorge Sztein (NIH, USA) and Jesús Martínez-Palacio (CIEMAT, Madrid, Spain). Second, the ISTT will be participating as exhibitor and will attend the 2013 FELASA meeting. The ISTT will have a booth in Barcelona (#230), manned by the ISTT administrative assistant, Alison Cameron, and where all ISTT members (and non-ISTT members) are welcome to visiting us. Finally, immediately next, our ISTT colleagues from The Netherlands, Marian Van Roon (VU, Amsterdam) and Sjef Verbeek (LUMC, Leiden), have organized their 2013 Workshop on Innovative Mouse Models (IMM2013). This 7th Workshop on Innovative Mouse Models will be held on 13-14 June 2013, at the Leiden University Medical Center, LUMC, Leiden, The Netherlands, and the ISTT will be co-sponsoring also this event. ISTT members will be entitled to reduced registrations at all these events, proudly co-sponsored by the ISTT.
The TT2013 meeting report, written by Douglas Strathdee (Beatson Institute for Cancer Research, Glasgow, Scotland, UK) and C. Bruce A. Whitelaw (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) has just been published, online, at the Transgenic Research journal web site. This review, entitled ‘TT2013 meeting report: the Transgenic Technology meeting visits Asia for the first time‘ nicely summarizes the talks and activities held during the recent 11th Transgenic Technology meeting, held in Guangzhou (China), on February 25-27, 2013, along with the subsequent hands-on workshop that was organized, on February 28-March 2, 2013. Douglas and Bruce, together with Peter Hohenstein (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) are the Organizers of the next 12th Transgenic Technology meeting, TT2014, which will be held in Edinburgh (Scotland, UK) on October 6-8, 2014.
Mouse sperm cryopreservation: satellite workshop at the 2013 FELASA-SECAL Congress in Barcelona, Spain, 10 June 2013Tuesday, March 26th, 2013
The International Society for Transgenic Technologies (ISTT) is pleased to announce the approved co-sponsorship of the Mouse Sperm Cryopreservation satellite workshop, organized by Jorge Sztein (NIH, Rockville, MD, USA) and Jesús Martínez Palacio (CIEMAT, Madrid, Spain), both ISTT members, in Barcelona (Spain) on 10 June 2013, within the activities associated to the 2013 FELASA-SECAL Congress in Barcelona, Spain, 10-13 June 2013. The main objective of this workshop is to acquaint students on reliable methods of mouse sperm cryopreservation, Jax and Nakagata, without requiring appliances or large investments to establishing a program at their centers. This course involves manipulation of animals and LN2.
This half-day satellite workshop will be held on 10 June 2013 from 09.00 to 12.00 h. at the Faculty of Medicine, University of Barcelona, Experimental Animal Unit. Satellite workshop registration fee: €120. Seats are limited to 24 participants. ISTT members, including those already registered to attend this workshop, are entitled to 25% discount. Interested participants should contact: firstname.lastname@example.org