Archive for the ‘rat’ Category

Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Sunday, July 27th, 2014
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Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Transgenic Animal Technology. A Laboratory Handbook (3rd edition, 2014)

Twenty years after the publication of the first edition and twelve years after the release of the second edition of this book, Carl A. Pinkert (Auburn University, College of Veterinary Medicine, Auburn, AL, USA) in association with Elsevier, releases now the third edition of his famous transgenic manual: “Transgenic Animal Technology. A Laboratory Handbook. 3rd edition, 2014“. As it will be familiar to readers of the two previous editions of this useful and unique handbook, this is not only a manual to understand how to make a transgenic mouse. This handbook looks beyond mice and it contains protocols to prepare a wide variety of genetically-modified animals, including: rats, rabbits, poultry, fish, pigs, ruminants and non-human primates. In addition, this compilation of helpful methods includes a number of chapters devoted to understand and improve all steps of transgenesis, from vector design, analysis of transgene integration and the evaluation of transgene expression. Finally, the book also includes cryopreservation methods, an embryo culture section, a review of standard nomenclature and a selection of databases and internet resources currently available in the field.

This handbook is a worth addition to any library, laboratory or transgenic facility, complementary to other available manuals on the subject, but unique in the sense that it exquisitely illustrates current transgenic methods that can be applied to a wide variety of animal species, beyond mice.

Carl A. Pinkert has been helped in his outstanding Editorial task by a large group of co-authors, experts in their subjects, including some ISTT members: Satoshi Akagi, Anna V. Anagnostopoulos, Benjamin P. Beaton, Cory F. Brayton, Steve Brown, Anthony W.S. Chan, Tom Doetschman, Rex A. Dunham, David A. Dunn, Janan T. Eppig, Almudena Fernandez, Tatiana Flisikowska, Vasiliy Galat, Robert A. Godke, Philip Iannaccone, Michael H. Irwin, Larry W. Johnson, Yoko Kato, Teoan Kim, Alexander Kind, Bon Chul Koo, Mo Sun Kwon, Daniel J. Ledbetter, Michael J. Martin, Kazutsugu Matsukawa, Colin McKerlie, Lluis Montoliu, Paul E. Mozdziak, Akira Onishi, Paul A. Overbeek, James N. Petitte, L. Philip Sanford, Jorge A. Piedrahita, Wendy K. Pogozelski, H. Greg Polites, Edmund B. Rucker III, Marina Sansinena, Angelika Schnieke, Kumiko Takeda, James A. Thomson, Ian A. Trounce, Yukio Tsunoda, Cristina Vicente-Garcia, Kevin D. Wells, Richard N. Winn and Curtis R. Youngs.

TT2014 abstracts: submission deadline is approaching (30 June)

Tuesday, June 17th, 2014
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TT2014 abstracts: submission deadline is approaching (30 June)

TT2014 abstracts: submission deadline is approaching (30 June)

From the International Society for Transgenic Technologies (ISTT) we warmly invite and encourage you all to submit your most recent and exciting results and developments in animal transgenesis to be presented at the forthcoming 12th Transgenic Technology (TT2014) meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014. Deadline for submitting abstracts for the TT2014 meeting is June 30.
To submit an abstract please visit this TT2014 meeting web page.

All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements:

  • Title (max. 25 words)
  • Name authors and affiliations (first author is the presenting author).
  • Text of the communication (max. 400 words).
  • Abstracts should be submitted no later than June 30, 2014.

Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards.

Best Poster Awards
All posters presented at the TT2014 meeting will be eligible for one of the ISTT Best Poster Awards, generously sponsored by Charles River, Inc.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

We are looking forward to receiving your exciting works to discuss the latest development on animal transgenesis!. See you all in Edinburgh!

Highlights of the TT2014 meeting in Edinburgh: a conference you can’t miss!

Thursday, June 5th, 2014
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The TT2014 meeting in Edinburgh (6-8 October 2014)

The TT2014 meeting in Edinburgh (6-8 October 2014)

This year’s ISTT main activity is the 12th Transgenic Technology conference, the TT2014 meeting, which will be held in Edinburgh, Scotland, UK, on 6-8 October 2014, followed by a 2-day hands-on workshop on basic zebrafish transgenesis techniques. The ISTT promotes the TT meetings every 18 months, these being the most important activity of our Society. This year, the local Organizers and advisory committees, commanded by Douglas Strathdee, need to be praised for preparing a most appealing and interesting program, addressing hot topics, current and most up-to-date issues actively discussed nowadays by the transgenic animal community. Talks that will be presented by the most active and prestigious scientists in our field.

Why you shouldn’t miss the TT2014 meeting?

  • If you are interested in the new transgenic methods associated to nucleases (ZFNs, TALENs and CRISPRs-Cas9) there will be plenty of  interesting talks where these new fantastic tools will be presented and discussed, directly by the key players in this rapidly-evolving field, including: Rudolf Jaenisch, William Skarnes, Angelika Schnieke, Kai Schönig, Ignacion Anegon, Pawel Pelczar, Francis Stewart, Keith Joung and Feng Zhang. And, most likely, these techniques will be referred and cited in many additional talks too, including the round table discussion about the future of transgenic core facilities, chaired by James Bussell.
  • If you are interested in ES cell biology and in innovative uses of ES cells and associated technologies there will be unique talks delivered by Jos Jonkers, Austin Smith, Ian Chambers, Janet Rossant and Alex Joyner
  • If you are interested in phenotyping your mouse animal models there will be fantastic talks delivered by  Jacqueline White, Stephen Murray, David Adams, Daniel MurphyAnna-Katerina Hadjatonakis and Vasilis Ntziachristos
  • If you are interested in non-rodent, large mammals and birds, animal models there will be great talks by James Murray, Angelika Schnieke, Mike McGrew and Adrian Shermann
  • If you are interested in rats there will be compelling talks by Kai Schönig and Ignacio Anegon
  • If you are interested in zebrafish animal models there will be fascinating talks by Stephen Ekker, Koichi KawakamiKeith Joung and Elizabeth Patton
  • If you are interested in animal welfare and 3Rs, in the best use of our laboratory animals, there will be captivating talks by Peter Hohenstein, Sara Wells and Jan-Bas Prins.

Therefore, there will be really engaging talks interesting to everyone in our field. This is why you shouldn’t miss this great and unique opportunity!.

Register now for the TT2014 meeting. Submission of abstracts will be accepted up to June 30. Early-Bird registration at reduced fees will be promoted up to July 31. ISTT members are entitled to reduced fee registration.

See you all in Edinburgh in October!

 

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

Sunday, May 11th, 2014
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Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

Submit your abstract(s) to the TT2014 meeting in Edinburgh: 30 June

The submission of abstracts for the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh (Scotland, UK), on 6-8 October, is OPEN. All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Authors are requested to submit an abstract with the following requirements: Title (max. 25 words), Name authors and affiliations (first author is the presenting author), and, Text of the communication (max. 400 words). Abstracts should be submitted no later than June 30, 2014. Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

Posters
Posters will be on display in the exhibition area throughout the duration of the meeting. Poster boards are 1.00m wide x 2.00m high and we recommend posters do not exceed 1.50m in length. A supply of Velcro tabs will be available at the venue. No screws or double-sided adhesive tape will be allowed due to the damage they can cause to the boards. All presented Posters at the TT2014 meeting will be entitled to the Best Poster Awards, generously sponsored by Charles River.

Oral Presentations
A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Should you be interested in being considered to speak at the meeting please select the appropriate option when submitting your abstract.

Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

More than 27,000 messages on animal transgenesis available to ISTT members through ISTT_list and tg-l archives

Sunday, February 9th, 2014
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More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

One of the most important assets of the International Society for Transgenic Technologies (ISTT), is the amount of information on animal transgenesis accummulated through the archives of the ISTT_list and tg-l email lists. Currently, more than 27,000 messages are fully available to ISTT members, conveniently organized in searchable and dynamic archives. The traditional transgenic-list (tg-l), operative since 1996 and offered from the ISTT web server since the end of 2011, has distributed over 22,000 messages since then, whereas the ISTT_list, associated and born with our Society in 2006, has disseminated some 5,000 messages, discussing both lists on almost each and every topic, issue or situation related directly or indirectly with animal transgenesis. All this endless information resource is fully available to ISTT members, through powerful search engines. Non-ISTT members subscribing to tg-l have access only to the most recent messages distributed through the tg-l, using the simple search engine, which allows simple searches and outputs the 50 most recent messages discussed on the subject of interest. In contrast, ISTT members have access to more sophysticated searching engines and the output always contains all messages archived on the matter investigated.

Obtaining granted access to these rich sources of information is very easy and cheap. Simply apply for ISTT membership! Submit now your application to become a member of the ISTT and you will get immediate and full access to all these messages.

Course on “Genetics of Laboratory Rodents”, Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Saturday, June 4th, 2011
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Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

The International Society for Transgenic Technologies (ISTT) is pleased to announce the co-sponsorship of the second edition of the International Course on “Genetics of Laboratory Rodents“, to be held at the Institut Pasteur de Montevideo (IPMONT), Uruguay, on 5-14 December 2011. This course is organized by Martina Crispo (IPMONT, ISTT member) and co-organized by Jean Jacques Panthier (Inst. Pasteur Paris). Following a most successful first edition in 2008, the aim of this course is to offer a training opportunity to South American research scientists and veterinarians in charge of laboratory animal facilities, in the most prominent areas of mammalian genetics (mostly mouse). This course offers an opportunity to receive an intensive training and get in touch with scientist of the region working in the same fields of interest.

The invited speakers at this international 2011 course on “Genetics of Laboratory Rodents” include the following experts in the field:

  • Jean Jacques Panthier (Institut Pasteur de Paris, France)
  • Xavier Montagutelli (Institut Pasteur de Paris, France)
  • Jean Louis Guénet (Institut Pasteur de Paris, France)
  • Jean Jaubert (Institut Pasteur de Paris, France) – ISTT  member
  • Michel Cohen Tannoudji (Institut Pasteur de Paris, France)
  • Ignacio Anegón (Institut de Transplantation-Urologie-Néphrologie,France) – ISTT member
  • Marcelo Rubinstein (Facultad de Ciencias Exactas y Naturales, UBA, Argentina)
  • Andreia Salgado (CEMIB, UNICAMP, Brazil)
  • Fernando Benavides (MD Anderson Cancer Center, Texas, USA) – ISTT member
  • Mariela Bollati (Institut Pasteur de Montevideo)
  • Martina Crispo (Institut Pasteur de Montevideo) – ISTT member

Interested participants should sent their applications by email to: curso-genetica-raton@pasteur.edu.uy by September 25, 2011, and include their CV, letter of motivation and letter of support of their advisors, all in PDF format. Two seats are reserved for qualifying ISTT members. There is no registration fee for this course. The 2011 International Course on “Genetics of Laboratory Rodents” is generously sponsored by CABBIO, AMSUD PASTEUR, Institut Pasteur de Montevideo, ISTT and  Ambassade de France en Uruguay.

TT2011 meeting: abstracts and awards deadlines coming soon

Monday, May 30th, 2011
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TT2011 Meeting: 24-26 October 2011, Trade Winds Island Grand Resort, St Pete Beach, Florida, USA

TT2011 Meeting: 24-26 October 2011, Trade Winds Island Grand Resort, St Pete Beach, Florida, USA

The 10th Transgenic Technology meeting (TT2011) will be held at the Trade Winds Island Grand Resort, in St Pete Beach, Florida, USA, on 24-26 October 2011, organized by the International Society for Transgenic Technologies (ISTT). The first deadline for submitting abstracts and applying for the various awards (Registration and Young Investigator Awards) will be due in about one month, on 30th June 2011. Accepted abstracts will be published in the scientific journal Transgenic Research, associated with the ISTT. Results from the various awards will be communicated by 16th July 2011.

Please, mark the TT2011 meeting in your agendas and don’t miss this opportunity to discuss the latest developments on Transgenic Technologies, at the International Level, with the world experts in the field. Looking froward to receive your latest work, experiments, designs, developments, your hottest research achievements using genetically modified animals.

Advanced Protocols for Animal Transgenesis. An ISTT Manual (Due: August 2011)

Saturday, April 9th, 2011
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Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.

This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Table of contents:

  • Karen S. Canady: Patent and licensing issues in transgenic technology.
  • Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
  • Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
  • Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
  • Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
  • Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
  • Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache  and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
  • Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
  • Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
  • Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
  • Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
  • Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
  • Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
  • Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
  • Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
  • Marina Gertsenstein: Tetraploid Complementation Assay.
  • Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
  • Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
  • Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
  • Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
  • Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
  • Karen Brennan: Colony Management.
  • Belen Pintado and Juan Hourcade: Cryopreservation.
  • Shirley Pease: Shipment of Mice and Embryos.
  • Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
  • Jan Parker-Thornburg: Refinement, Reduction and Replacement

ISTT Members are entitled to a 33% discount on the book price.

Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

Friday, January 7th, 2011
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Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

For the third time, the  Nantes “Transgenic rats” core facility (Institut Fédératif de Recherche 26, OUEST-Génopole and IBiSA) organizes a 1-day meeting (June 6, 2011) on animal transgenesis. This year, the organizers (Ignacio Anegon and Sévérine Menoret, both ISTT members; along with Séverine Rémy, Laurent Tesson, Claire Usal, Tuan. H.  Nguyen, as local Organizers) have entitled this meeting “Transgenesis and genome analysis“. The International Society for Transgenic Technologies (ISTT) is most happy to support and co-sponsor again this well-focused 1-day meeting on transgenesis. This year, there will be an additional benefit for all those ISTT members willing to attend to this meeting: ISTT members will be entitled to a reduced (half) registration fee. The scientific program includes talks on Transposons, Meganucleases, Zn-finger nucleases, vector design, lentiviral vectors, integration site, integrative genomics, transgenic rodent models for hypertension research, transgenic pigs for xenotransplantion, comparative genomics, rat ES cells and human iPS and ES cells.

Registration forms are available from the meeting web site. Interested participants are requested to register by May 25, 2011.

ISTT and Transgenic Research jointly promote the use of standard nomenclature

Tuesday, December 7th, 2010
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Examples of use of standard nomenclature [see Montoliu & Whitelaw (2010) for a full descrption]

Examples of use of standard nomenclature: see Montoliu & Whitelaw (2010) for a full descrption

The International Society for Transgenic Technologies (ISTT) and the scientific journal, Transgenic Research, to which the ISTT is associated, have decided to promote the use of standard nomenclature for naming mouse strains, genes, mutations and alleles. In this regard, and according to ISTT aims and activities, ISTT will disseminate in its web site, blog, newsletter and Transgenic Technology (TT) meetings, information and recommendations regarding how to use standard nomenclature. Likewise, the correct use of rules and guidelines for standard nomenclature for naming mouse strains, genes, mutations and alleles will be enforced in subsequent articles submitted to Transgenic Research for publication, as it is already done in several other journals.

Joint Position Article (ISTT and Transgenic Research):

Montoliu Lluis & Whitelaw C. Bruce A. (2010) Using standard nomenclature to adequately name transgenes, knockout gene alleles and any mutation associated to a genetically modified mouse strain. Transgenic Research (ePub, 16 July 2010) [this article has been made freely available by Springer]


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