Archive for the ‘rat’ Category

More than 27,000 messages on animal transgenesis available to ISTT members through ISTT_list and tg-l archives

Sunday, February 9th, 2014
LinkedIn
More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

More than 27,000 messages on animal transgenesis available through ISTT_list and tg-l archives

One of the most important assets of the International Society for Transgenic Technologies (ISTT), is the amount of information on animal transgenesis accummulated through the archives of the ISTT_list and tg-l email lists. Currently, more than 27,000 messages are fully available to ISTT members, conveniently organized in searchable and dynamic archives. The traditional transgenic-list (tg-l), operative since 1996 and offered from the ISTT web server since the end of 2011, has distributed over 22,000 messages since then, whereas the ISTT_list, associated and born with our Society in 2006, has disseminated some 5,000 messages, discussing both lists on almost each and every topic, issue or situation related directly or indirectly with animal transgenesis. All this endless information resource is fully available to ISTT members, through powerful search engines. Non-ISTT members subscribing to tg-l have access only to the most recent messages distributed through the tg-l, using the simple search engine, which allows simple searches and outputs the 50 most recent messages discussed on the subject of interest. In contrast, ISTT members have access to more sophysticated searching engines and the output always contains all messages archived on the matter investigated.

Obtaining granted access to these rich sources of information is very easy and cheap. Simply apply for ISTT membership! Submit now your application to become a member of the ISTT and you will get immediate and full access to all these messages.

Course on “Genetics of Laboratory Rodents”, Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Saturday, June 4th, 2011
LinkedIn
Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

The International Society for Transgenic Technologies (ISTT) is pleased to announce the co-sponsorship of the second edition of the International Course on “Genetics of Laboratory Rodents“, to be held at the Institut Pasteur de Montevideo (IPMONT), Uruguay, on 5-14 December 2011. This course is organized by Martina Crispo (IPMONT, ISTT member) and co-organized by Jean Jacques Panthier (Inst. Pasteur Paris). Following a most successful first edition in 2008, the aim of this course is to offer a training opportunity to South American research scientists and veterinarians in charge of laboratory animal facilities, in the most prominent areas of mammalian genetics (mostly mouse). This course offers an opportunity to receive an intensive training and get in touch with scientist of the region working in the same fields of interest.

The invited speakers at this international 2011 course on “Genetics of Laboratory Rodents” include the following experts in the field:

  • Jean Jacques Panthier (Institut Pasteur de Paris, France)
  • Xavier Montagutelli (Institut Pasteur de Paris, France)
  • Jean Louis Guénet (Institut Pasteur de Paris, France)
  • Jean Jaubert (Institut Pasteur de Paris, France) – ISTT  member
  • Michel Cohen Tannoudji (Institut Pasteur de Paris, France)
  • Ignacio Anegón (Institut de Transplantation-Urologie-Néphrologie,France) – ISTT member
  • Marcelo Rubinstein (Facultad de Ciencias Exactas y Naturales, UBA, Argentina)
  • Andreia Salgado (CEMIB, UNICAMP, Brazil)
  • Fernando Benavides (MD Anderson Cancer Center, Texas, USA) – ISTT member
  • Mariela Bollati (Institut Pasteur de Montevideo)
  • Martina Crispo (Institut Pasteur de Montevideo) – ISTT member

Interested participants should sent their applications by email to: curso-genetica-raton@pasteur.edu.uy by September 25, 2011, and include their CV, letter of motivation and letter of support of their advisors, all in PDF format. Two seats are reserved for qualifying ISTT members. There is no registration fee for this course. The 2011 International Course on “Genetics of Laboratory Rodents” is generously sponsored by CABBIO, AMSUD PASTEUR, Institut Pasteur de Montevideo, ISTT and  Ambassade de France en Uruguay.

TT2011 meeting: abstracts and awards deadlines coming soon

Monday, May 30th, 2011
LinkedIn
TT2011 Meeting: 24-26 October 2011, Trade Winds Island Grand Resort, St Pete Beach, Florida, USA

TT2011 Meeting: 24-26 October 2011, Trade Winds Island Grand Resort, St Pete Beach, Florida, USA

The 10th Transgenic Technology meeting (TT2011) will be held at the Trade Winds Island Grand Resort, in St Pete Beach, Florida, USA, on 24-26 October 2011, organized by the International Society for Transgenic Technologies (ISTT). The first deadline for submitting abstracts and applying for the various awards (Registration and Young Investigator Awards) will be due in about one month, on 30th June 2011. Accepted abstracts will be published in the scientific journal Transgenic Research, associated with the ISTT. Results from the various awards will be communicated by 16th July 2011.

Please, mark the TT2011 meeting in your agendas and don’t miss this opportunity to discuss the latest developments on Transgenic Technologies, at the International Level, with the world experts in the field. Looking froward to receive your latest work, experiments, designs, developments, your hottest research achievements using genetically modified animals.

Advanced Protocols for Animal Transgenesis. An ISTT Manual (Due: August 2011)

Saturday, April 9th, 2011
LinkedIn
Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.

This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Table of contents:

  • Karen S. Canady: Patent and licensing issues in transgenic technology.
  • Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
  • Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
  • Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
  • Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
  • Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
  • Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache  and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
  • Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
  • Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
  • Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
  • Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
  • Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
  • Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
  • Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
  • Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
  • Marina Gertsenstein: Tetraploid Complementation Assay.
  • Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
  • Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
  • Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
  • Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
  • Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
  • Karen Brennan: Colony Management.
  • Belen Pintado and Juan Hourcade: Cryopreservation.
  • Shirley Pease: Shipment of Mice and Embryos.
  • Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
  • Jan Parker-Thornburg: Refinement, Reduction and Replacement

ISTT Members are entitled to a 33% discount on the book price.

Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

Friday, January 7th, 2011
LinkedIn
Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

Meeting: Transgenesis and genome analysis, Nantes (France), June 6, 2011

For the third time, the  Nantes “Transgenic rats” core facility (Institut Fédératif de Recherche 26, OUEST-Génopole and IBiSA) organizes a 1-day meeting (June 6, 2011) on animal transgenesis. This year, the organizers (Ignacio Anegon and Sévérine Menoret, both ISTT members; along with Séverine Rémy, Laurent Tesson, Claire Usal, Tuan. H.  Nguyen, as local Organizers) have entitled this meeting “Transgenesis and genome analysis“. The International Society for Transgenic Technologies (ISTT) is most happy to support and co-sponsor again this well-focused 1-day meeting on transgenesis. This year, there will be an additional benefit for all those ISTT members willing to attend to this meeting: ISTT members will be entitled to a reduced (half) registration fee. The scientific program includes talks on Transposons, Meganucleases, Zn-finger nucleases, vector design, lentiviral vectors, integration site, integrative genomics, transgenic rodent models for hypertension research, transgenic pigs for xenotransplantion, comparative genomics, rat ES cells and human iPS and ES cells.

Registration forms are available from the meeting web site. Interested participants are requested to register by May 25, 2011.

ISTT and Transgenic Research jointly promote the use of standard nomenclature

Tuesday, December 7th, 2010
LinkedIn

Examples of use of standard nomenclature [see Montoliu & Whitelaw (2010) for a full descrption]

Examples of use of standard nomenclature: see Montoliu & Whitelaw (2010) for a full descrption

The International Society for Transgenic Technologies (ISTT) and the scientific journal, Transgenic Research, to which the ISTT is associated, have decided to promote the use of standard nomenclature for naming mouse strains, genes, mutations and alleles. In this regard, and according to ISTT aims and activities, ISTT will disseminate in its web site, blog, newsletter and Transgenic Technology (TT) meetings, information and recommendations regarding how to use standard nomenclature. Likewise, the correct use of rules and guidelines for standard nomenclature for naming mouse strains, genes, mutations and alleles will be enforced in subsequent articles submitted to Transgenic Research for publication, as it is already done in several other journals.

Joint Position Article (ISTT and Transgenic Research):

Montoliu Lluis & Whitelaw C. Bruce A. (2010) Using standard nomenclature to adequately name transgenes, knockout gene alleles and any mutation associated to a genetically modified mouse strain. Transgenic Research (ePub, 16 July 2010) [this article has been made freely available by Springer]

KO rats obtained by homologous recombination in rat ES cells

Saturday, August 21st, 2010
LinkedIn
KO rats obtained by homologous recombination in rat ES cells

KO rats obtained by homologous recombination in rat ES cells

One year ago, the first KO rats were reported by Geurts et al. (Science, 2009, 325:433) , using the Zn-finger nucleases technology.  Half a year earlier, several laboratories had reported the pionner isolation of true rat embryonic stems (ES) cells, including a manuscript published by Li et al. (Cell, 2008, 135: 1299-1310), from Qi-Long Ying’s lab. Last week, this laboratory reported the generation of the first knockout rats obtained by homologous recombination in rat ES cells [Tong et al. (Nature, 2010, August 11)], thereby illustrating the power of those rat ES cells for the inactivation of a most popular locus, the P53 gene. This report demonstrates that the rat genome is amenable to the same type of genetic modifications as the mouse genome, using equivalent procedures.

First targeted Knockout Rats obtained by Zinc Finger Nuclease technology

Friday, July 24th, 2009
LinkedIn
KnockOut Rats Obtained by ZFN technology

KnockOut Rats Obtained by ZFN technology

Science magazine publishes tody a report by Geurts et al. describing the first successful attempt to produce gene targeted knockout rats through the use of Zinc Finger Nuclease technology, developed by Sangamo BioSciences, Inc. This strategy does not require the use of embryonic stem (ES) cells. Targeted mutations are induced by standard microinjection of DNA and RNA molecules into 1-cell rat embryos. This milestone has been achieved by a collaborative effort of academic and industrial scientists coordinated by the biotechnology company Open Monoclonal Technology, Inc. The team includes two members of ISTT, Ignacio Anegon and Séverine Ménoret, from the Transgenic Rats Common Facility at INSERM, UMR 643, CHU, Nantes, Université de Nantes, France. Ignacio Anegon has confirmed his participation, as invited speaker, at the forthcoming TT2010 meeting, to be held in Berlin, March 22-24, 2010.

In this work, OMT scientists and their collaborators have made use of zinc finger nuclease strategies to target an integrated reporter and two endogenous rat genes, Immunoglobulin M (IgM) and Rab38.  The mutations are induced at high frequency (animals carry 25 to 100% disruption at the target locus) by standard microinjection of DNA or RNA molecules encoding specific Zinc Finger Nucleases. These mutations are faithfully and efficiently transmitted through the germline. Quoting the authors: “…Our data demonstrate the feasibility of targeted gene disruption in multiple rat strains within 4 months time, paving the way to a humanized monoclonal antibody platform and additional human disease models.”

Nantes 2009: meeting report

Wednesday, June 24th, 2009
LinkedIn
Nantes meeting on Transgenic Animals

Nantes meeting on Transgenic Animals

On June 8 2009 was held in Nantes, France, the international meeting “Transgenesis ; recent technical developments and applications”. This meeting is the second one of its kind to be organized by the Transgenic Rats Nantes facility from the INSERM UMR 643 and Biogenouest. The meeting was supported by several academic institutions, as well as private companies. The meeting received support and co-sponsorship from the International Society for Transgenic Technology (ISTT). Around 100 participants, from France but also in significant proportion from different European countries and Canada, attended the meeting and registration prices were kept as low as possible to facilitate the participation of students.

The meeting aimed to provide an update on recent technical developments in the generation of transgenic animals and in some of their applications. It was intended for Master, PhD and medical students with a background in molecular biology and genetics as an introduction to future work in these rapidly developing areas of research. It was also intended for post-docs and scientists already working in certain of these fields and who are interested in expanding their knowledge on the potential applications of these new techniques to their models. Here, you can download the corresponding meeting report.

Rat ES cells obtained

Friday, January 2nd, 2009
LinkedIn
Rat ES cells have been obtained

Rat ES cells have been obtained

The end of the past year included an outstanding experiment published on December 26, in Cell, from Austin Smith’s laboratory in Cambridge, UK.

Capture of authentic embryonic stem cells from rat blastocysts. Buehr M, Meek S, Blair K, Yang J, Ure J, Silva J, McLay R, Hall J, Ying QL, Smith A. Cell. 2008 Dec 26;135(7):1287-98.

Indeed, the group of Austin Smith, succeeded to obtain the first rat ES cells capable of colonising somatic and germ-line tissues, capable of forming chimeras, and capable of transmitting their genome to the progeny. The key of this achievement, in contrast to many previous attempts by various groups, has been the use of a combination of defined specific inhibitors (3i/2i) in the presence of LIF. Specially successful was the combination of 2i (inhibitors of the MEK and GSK3 pathways)+LIF. Even though the success rate is still rather low it lays the ground for future experiments in order to find the most adequate combinations of rat ES-cell donors and rat blastocysts for optimal results.

In 2003 we learnt about the cloning of rats by an experiment conducted in collaboration between Chinese and French scientists, commanded by Jean Paul Renard and first authored by Qi Zhou, awarded the TT Prize in Uppsala, at the TT2004 meeting (Zhou et al. Science 2003, Nov 14;302(5648):1179). It was envisaged this could be a way to overcome the limitation of a lack of rat ES cells for the production of specific rat knock-out strains. However, that experiment has proven very difficult to reproduce. Now, with this achievement by Austin Smith’s lab, and after eventually finding the optimal rat strain combinations and tissue culture conditions, it appears more feasible the possibility of preparing a variety of specific rat KOs.

In summary, a paper worth reading and discussing in laboratories and transgenic facilities. While reading this paper, it is particularly interesting how much they could learn from failure experiments and how they eventually came across a combination of factors that resulted in the generation of germ-line transmission capable rat ES cells.

Rat picture obtained from this WEB.


This blog is protected by Dave\'s Spam Karma 2: 2704 Spams eaten and counting...