The International Society for Transgenic Technologies (ISTT) will soon move its headquarters from Europe to America, from Spain (Europe Union) to the State of New York (United States of America). After the 12th Transgenic Technology (TT) meeting in Edinburgh, TT2014, the ISTT Society will be legally represented by a new non-profit entity: ISTT, Inc., registered in the State of New York (USA) in May 2013, after the corresponding approval was obtained from ISTT members at the Extraordinary General Assembly held at the TT2013 meeting, in Guangzhou, China, in February 2013. This moving process was essentially undertaken to allow any member to run for any position at the Board and to reduce tax running costs. The process is meant to be smooth and transparent to all members which will be kindly requested to join the new entity, to approve the new bylaws and to approve the new Board. Everything else will remain the same and the ISTT members will continue enjoying the same benefits from being part of the ISTT Society. The ISTT thank all of its members for their support and collaboration.
Archive for the ‘ISTT Members’ Category
From the International Society of Transgenic Technologies (ISTT), it is our pleasure to announce that the next, 13th, Transgenic Technology (TT) meeting, TT2016, will be held in Prague (Czech Republic), in March 2016, organized by Dr. Radislav Sedlacek (Associate Professor, Institute of Molecular Genetics; Head of Laboratory of Transgenic Models of Diseases; Project Director, Czech Centre for Phenogenomics; ISTT Member).
The ISTT was delighted to accept a very appealing and well-structured meeting proposal from Prague, a historical city lying in the heart of Europe. Prague is a beautiful city with magical atmosphere. It offers historical as well as modern hotels, restaurants, infrastructure and places of sightseeing. TT2016 conference delegates arriving in Prague will enjoy a high standard of hospitality at very reasonable prices.
The TT2016 meeting web site (http://www.transtechsociety.org/tt2016) and the official TT2016 meeting address (email@example.com) are already operative. Dr. Radislav Sedlacek will present the TT2016 meeting in Edinburgh, at the TT2014 meeting, introducing his team of collaborators, the organizing and advisory committees, their view, plans and expectations for this transgenic technology meeting, where again, and with the support of the ISTT, the Organizers will prepare a very attractive scientific program with all the newest techniques, applications and approaches developed in the field of animal transgenesis.
The TT2016 meeting will he held in Prague in March 2016. The exact dates and location of the conference venue will be communicated shortly.
This is yet another interesting book in our field that has been published this year, 2014. This manual, entitled “Mouse Genetics. Methods and Protocols“, edited by Shree Ram Singh and Vinzenzo Coppola, in association with the Publisher, Humana Press/Springer, contains a collection of useful protocols covering most of the methods that can be currently applied for the genetic modification of the mouse genome. According to its presentation at the Springer web page, this book “provides selected mouse genetic techniques and their application in modeling varieties of human diseases. The chapters are mainly focused on the generation of different transgenic mice to accomplish the manipulation of genes of interest, tracing cell lineages, and modeling human diseases. (…) Each chapter contains a brief introduction, a list of necessary materials, systematic, readily reproducible methods, and a notes section, which shares tips on troubleshooting in order to avoid known pitfalls.”
The table of contents of this book illustrates the variety of highly sophysticated methods, beyond standard techniques, that are discussed here in detail, around mouse genetics, including: pronuclear injection-based targeted transgenesis through Cre-loxP specific recombination, the use of recombinase-mediated cassette exchange (RMCE) strategies, several approaches for preparing and analyzing conditional mutant alleles using tamoxifen-dependent Cre recombinases, the use of ICSI for the generation of transgenic mice, the use of BACs, mosaic analysis with double markers (MADM) in mice, transposon-mediated transgenesis, overexpression of microRNAs using Rosa26-mediated recombination, the isolation of various somatic and pluripotent cells, the generation of transgenic mice through spermatogonial stem cells in vivo, and, several illustrative examples of how different mouse engineered animal models are best suited to study a variety of human diseases. Hence, this book is also complementary to other recently published manuals, since it contains a careful detailed description of new methods that are not been covered in other similar titles in the field.
The editors of this book, Shree Ram Singh and Vinzenzo Coppola, have counted with the generous expertise shared and provided by a very large list of co-authors, including some ISTT members: Masato Ohtsuka, Kazuhito Sakamoto, Channabasavaiah B. Gurumurthy, Kay-Uwe Wagner, Petra Kraus, V. Sivakamasundari, Xing Xing, Thomas Lufkin, Anton J.M. Roebroek, Bart Van Gool, Kun-Hsiung Lee, Susanne Feil, Jana Krauss, Martin Thunemann, Robert Feil, Pedro N. Moreira, Lluis Montoliu, Jane Beil, Thorsten Buch, Sheng Ding, Tian Xu, Xiaohui Wu, Hui Zong, Claudia Piovan, Foued Amari, Francesca Lovat, Qun Chen, Olga Simmons, Esther M. Bolanis, Jian Wang, Simon J. Conway, Kanika Jain, Paul J. Verma, Jun Liu, Pollyanna Agnes Goh, Michael D. Williams, Wilson Wong, Amanda Rixon, Sarang N. Satoor, Anandwardhan A. Hardikar, Mugdha V. Joglekar, Andrei M. Vacaru, Joseph Vitale, Johnathan Nieves, Margaret H. Baron, Kristbjorn Orri Gudmundsson, Kevin Oakley, Yufen Han, Yang Du, Lalit Sehgal, Abul Usmani, Sorab N. Dalal, Subeer S. Majumdar, Spencer W. Luebben, Naoko Shima, Tsuyoshi Kawabata, Robert M. Hoffman, Viive M. Howell, Emily K. Colvin, Vishalakshi Chavali, Shyam Sundar Nandi, Paras Kumar Mishra, Julia Lorenz, Susanne Grässel, Ganesan Ramesh, Punithavathi Ranganathan, Santhakumar Manicassamy, Indumathi Manoharan, Deepak P. Patil, Holly D. Kristensen and Yogesh Shouche.
This new book will be added to the collection of Springer books published on animal transgenesis and animal genetics for which ISTT members are entitled to a 33% discount, as one of the many benefits associated with the ISTT membership.
On behalf of the International Society for Transgenic Technologies (ISTT), it is my pleasure to announce the results of the 2014 ISTT Elections. Jan Parker-Thornburg has been elected ISTT President. Aimee Stablewski has been elected ISTT Secretary and Treasurer. Cheryl Bock and Martina Crispo have been elected new ISTT Board members. Elizabeth Williams has been re-elected ISTT Board member for a second and last turn. Congratulations to all of them!. And our deepest appreciation to all candidates generously participating in this 2014 ISTT Election process. The new elect Officers and Board members will be validated by the ISTT General Assembly at the 12th Transgenic Technology meeting (TT2014), in Edinburgh, in October, and they will enter into service on January 1st, 2015, for a period of three consecutive TT meetings, TT2014-TT2017, as stated in the updated ISTT bylaws registered in the US. Best wishes and good luck to all.
A minimum of six registration awards for ISTT members willing to attend the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh, Scotland, UK, on 6-8 October 2014, will be sponsored by the International Society for Transgenic Technologies (ISTT). Applications should be sent, along with the registration confirmation and the requested additional documents to firstname.lastname@example.org by June 30, 2014. The ISTT will pay the Registration Fee of all applicants selected for one of these TT2014 registration awards. Please note that applicants not selected for an award are required to pay the corresponding registration fee. Please note the Award covers registration fees and attendance to all social events, however, does not cover travel, accommodation expenses or attendance at pre meeting events. Award decisions will be communicated by July 15, 2014 and awardees will receive a diploma at the TT2014 Meeting.
Applications for registration awards by ISTT members should be sent to the official ISTT email address (email@example.com), by June 30, 2014, and should include the following documentation:
- Applicant’s CV
- Letter describing how the applicant will benefit from attendance at the TT meeting
- Only for technician/students: Letter of support from the applicant’s head/supervisor stating the support to the applicant’s participation in the TT meeting and highlighting why attendance will benefit the applicant’s career
Applicants must register first at the TT2014 website and select, as a payment method, “application for registration awards”. The ISTT will pay the Registration Fee of all applicants selected for an award. However, if they are not selected they would be kindly requested to pay the corresponding registration fee
The ISTT Council will examine all applications received. Registration Awards will be selected according to the documents submitted and with preference given to:
- Student/Technician ISTT Members
- ISTT Members submitting an abstract for presentation as a poster/short-oral communication at the TT meeting
- Any other ISTT Member
Award decisions will be communicated by July 15, 2014. Awardees will have their TT registration fees entirely waived. Awardees will receive a diploma at the Awards ceremony at the end of the TT2014 meeting. Applicants that are not yet members of the ISTT may choose to register/join first the ISTT and simultaneously submit their travel/registration award application. ISTT Registration/Travel awards for each TT meeting will only be provided to ISTT members who have paid their current annual fees.
The 2014 ISTT Election Process has been launched. All members of the International Society for Transgenic Technologies are warmly invited to elect the next ISTT President, ISTT Secretary, ISTT Treasurer and three new board members. All these new positions at the ISTT Board will begin their term of service on January 1st, 2015. The 2014 ISTT elections will take place within the members-only area of the ISTT web site, during the entire month of June 2014. Results will be published shortly thereafter. The ISTT wishes to express its deepest gratitude and sincere acknowledgement to all 12 nominated candidates which will run for the various positions to be elected within the board. The official nominated candidates participating in this 2014 ISTT Election Process are: Jan Parker-Thornburg (USA), Lynn Doglio (USA), Channabasavaiah B Gurumurthy (USA), Aimee Stablewski (USA), Cheryl Bock (USA), Martina Crispo (Uruguay), Larry Johnson (USA), Sagrario Ortega (Spain), Xin Rairdan (USA), Tanya Templeton (Australia), Elizabeth Williams (Australia) and Branko Zevnik (Germany). All candidates deserve our most sincere appreciation for their generous willingness to serve and further contribute to the success of our Society. Good luck to everyone!
On Thursday I got the very sad information that Ueli (Ulrich) Märki passed away on Saturday last week. This is a great loss for all that got to know him and we all mourn with his family about this tragic loss.
I first got in contact to Ueli during my university time in Heidelberg in the beginning of the 90th, while he was still working for RCC in Switzerland. I got to know him as a very dedicated scientist (thanks for teaching me a lot of mouse genetics Ueli!) a very nice colleague and in the long-run as a dear friend. When we initiated the first Transgenic Symposium in Heidelberg in 1993, he was one of the supporting persons, encouraging us to get this meeting up and running.
During the forthcoming years I met Ueli at various meetings and it was always a pleasure and a lot of fun together with him. I especially enjoyed the unofficial parts at the meetings, which we spend with a good beer or wine, discussing all the world and his brother. Even when I moved over to Sweden and he joining Taconic we kept the contact even when we then didn’t meet so often. And it was again he helping me a lot with setting up the first TT Meeting in Stockholm 1999. He was also one of the first to join the ISTT when it was founded, always supporting us with good ideas, but also challenging us when something didn’t seemed to go the right way.
I was looking forward meeting him again at the next GV-SOLAS meeting this year in September. I am going to miss and will hold him in good remembrance, as valued researcher and dear friend.
Our deepest regrets and condolences go to his family and his close friends. We are going to miss him unspeakably.
CARD-RPCI Mouse Sperm and Embryo Cryopreservation Course, Roswell Park Cancer Institute, Buffalo, NY, USA, September 15th-19th, 2014Monday, March 31st, 2014
The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor another CARD Cryopreservation Course. This time, for the first time, the CARD methods will be taught in North America: the CARD-RPCI Mouse Sperm and Embryo Cryopreservation Practical Course will be held at Roswell Park Cancer Institute (RPCI) in Buffalo, NY USA. The course will be held September 15th-19th, 2014, and will be a hands-on intensive workshop.
The course is organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD), Aimee Stablewski (Roswell Park Cancer Institute, Buffalo, NY, USA, Co-Director of the Gene Targeting and Transgenic Resource) and Jan Parker-Thornburg (MD Anderson, Houston, TX, USA, Director of the Genetically Engineered Mouse Facility).
This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV, completion of this application questionnaire, and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to: firstname.lastname@example.org
The maximum number of participants attending this course will be 18, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted starting April 1st, 2014 and will close on May 31st, 2014. The review and selection of participants will be done by the Organizers from June 1st-15th, 2014. Registrations and payments for selected participants will be accepted from June 16th, 2014 to August 31st, 2014. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by August 31st, 2014 will be readily substituted by the first available person from this waiting list, starting on September 1st, 2014.
The course registration fee is $1300 USD (with a reduced fee of $1100 USD for ISTT members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a hotel on Roswell Park Cancer Institute’s campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.
COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines.
The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 17.
Hands-on topics that will be covered during this cryopreservation course:
- Isolating unfertilized mouse oocytes
- Isolating and cold storage/shipping of mouse cauda epididymis
- Isolating and cold storage of embryos
- Freezing/thawing mouse sperm and IVF using CARD frozen sperm and legacy sperm
- Fresh mouse sperm and IVF
- Cold Storage sperm and IVF
- Freezing/thawing 2-cell IVF-derived mouse embryos
- Vitrification of mouse oocytes and embryos
- IVF of vitrified mouse oocytes
- Ovary transplantation/ovary freezing
- Embryo transfer techniques in mice (oviduct, uterus via NSET)
Additional lectures on the following topics:
- Historic and Scientific perspectives of embryo and sperm cryopreservation
- Comparing current embryo and sperm cryopreservation methods
- Vitrification of oocytes and their use for IVF
- New US Guidelines for the use of animals in research/IACUC
- Cold storage and transport of germplasm
- Large archiving and distribution centers- challenges and solutions
- Shipping mice, refrigerated and frozen material
- Freezing and transplantation of ovaries
- NSET: non-surgical embryo transfer
- Breeding strategies for cohort generation of GEM’s
- Naomi Nakagata (CARD-Kumamoto University, Japan)
- Toru Takeo (CARD-Kumamoto University, Japan)
- Shuuji Tsuchiyama (CARD-Kumamoto University, Japan)
- Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
- Yukie Haruguchi (CARD-Kumamoto University, Japan)
- Tomoko Kondo (CARD-Kumamoto University, Japan)
- Yumi Takeshita (CARD-Kumamoto University, Japan)
- Yuko Nakamuta (CARD-Kumamoto University, Japan)
- Tomoko Umeno (CARD-Kumamoto University, Japan)
- Jan Parker-Thornburg (MD Anderson, Houston, TX, USA)
- Aimee Stablewski (Roswell Park Cancer Institute, Buffalo, NY, USA)
- Dawn Barnas (Roswell Park Cancer Institute, Buffalo, NY, USA)
- Amar Dasani (Taconic, Germantown, NY, USA)
- Andrea Dunn (Taconic, Germantown, NY, USA)
- Kristy Williams (USA)
- Barbara Stone (ParaTechs, Lexington, KY, USA)
- Jorge Sztein (Barcelona, Spain)
Additional lectures by:
- Naomi Nakagata (CARD-Kumamoto University, Japan)
- Toru Takeo (CARD-Kumamoto University, Japan)
- Sandra Sexton (Roswell Park Cancer Institute, Buffalo, NY, USA)
- Jorge Sztein (Barcelona, Spain)
- Barbara Stone (ParaTechs, Lexington, KY, USA)
- Lluis Montoliu (CNB-CSIC, Madrid, Spain)
- Carlisle Landel (Transposagen Inc., Lexington, KY, USA)
- Amar Dasani (Taconic, Germantown, NY, USA)
It is with great sadness that we note the passing of Stanley Leibo, who died this morning, March 25, in the USA. Stanley Leibo was Professor at the Department of Biological Sciences, University of New Orleans, LA, USA. On behalf of the ISTT, we wish to offer our most sincere condolences to his family and friends.
Stanley Leibo was a renowned authority in cryobiology and one of the pioneers in the cryopreservation of mammalian germplasm. His seminal publication in Science in 1972, in collaboration with Peter Mazur and David Whittingham, described the first, successful cryopreservation of mammalian embryos, demonstrating the recovery of live pups from mouse embryos that had been stored in liquid nitrogen and liquid helium. His scientific work laid the foundation for the current widespread use of cryopreservation by human fertility clinics, agriculture, scientific resource repositories, and conservation efforts preserving endangered species. In addition to his outstanding scientific contributions, Stanley Leibo will be remembered for the joy and passion he brought to teaching. He was a fixture from the beginning in the Jackson Laboratory’s Cryopreservation Course, offered both in Bar Harbor and later also in partnership with EMMA in Monterotondo, Italy, where over the years hundreds of students, including many ISTT members, had the pleasure of hearing his enthusiastic lectures on the history, theory and practice of cryopreserving mammalian cells and germplasm.
Stanley Leibo was a mentor for many of us, a colleague, and most of all, also a friend. We will not forget him. May he rest in peace.
P.S. Additional information from Stanley Leibo’s career and achievements can be obtained from this document, published in Reproduction, Fertiliy and Development, when he received the 2009 IETS Pioneer Award
The next ISTT meeting will be held in Europe this year. The 12th Transgenic Technology (TT2014) meeting, will take place in Edinburgh, Scotland, UK, on 6-8 October 2014, organized by ISTT members Douglas Strathdee (chair), Peter Hohenstein and Bruce Whitelaw, and hosted by three Scottish research institutes and the University of Edinburgh: the Roslin Institute; the Institute of Genetics and Molecular Medicine and the Beatson Institute for Cancer Research. The TT2014 meeting will be followed by the 2-day hands-on workshop “An Introduction to Zebrafish Transgenesis“, on 8-10 October 2014.
An outstanding group of invited speakers have confirmed their participation at the TT2014 meeting. Abstract submissions and application for the ISTT registration awards (for ISTT members) deadlines merge on 30 June 2014. Early bird registration deadline at reduced fees is 31 July 2014. A number of submitted abstracts will be selected for oral presentation on topics including:
- new technologies in transgenesis
- pluripotential stem cells
- targeted nucleases and genome editing
- models of human disease
- animal ethics and welfare
- large-scale phenotyping initiatives
- animal biotechnology
- in vivo imaging
- zebrafish models and transgenesis
Douglas Strathdee and his colleagues have prepared the following collection of eight Posters to advertise the TT2014 meeting, illustrated with beautiful Edinburgh pictures. Please, help us announcing and disseminating the TT2014 meeting by putting one or several of these Posters at your centres, institutions, facilities, departments, universities. The TT meeting is a unique forum occurring every 18 months where to discuss the latest technical developments and applications on animal transgenesis. This is a conference that can’t be missed by anyone interested in this subject! Thanks for helping us advertise the TT2014 meeting!