Archive for the ‘transgenic research’ Category
Saturday, August 21st, 2010
KO rats obtained by homologous recombination in rat ES cells
One year ago, the first KO rats were reported by Geurts et al. (Science, 2009, 325:433) , using the Zn-finger nucleases technology. Half a year earlier, several laboratories had reported the pionner isolation of true rat embryonic stems (ES) cells, including a manuscript published by Li et al. (Cell, 2008, 135: 1299-1310), from Qi-Long Ying’s lab. Last week, this laboratory reported the generation of the first knockout rats obtained by homologous recombination in rat ES cells [Tong et al. (Nature, 2010, August 11)], thereby illustrating the power of those rat ES cells for the inactivation of a most popular locus, the P53 gene. This report demonstrates that the rat genome is amenable to the same type of genetic modifications as the mouse genome, using equivalent procedures.
Tags: rat ES cells, rat KO
Posted in ES cells, general, information, knock-outs, knockout, publication, rat, transgenic research | No Comments »
Friday, July 16th, 2010
ES cells set for aggregation with a mouse morula
Marina Gertsenstein (member of ISTT) and her colleagues, from the Samuel Lunenfeld Research Institute, Mount Sinai Hospital and the Toronto Centre for Phenogenomics, in Toronto (ON, Canada), have recently published a scientific article in PLoS ONE describing the efficient generation of mouse chimeras with C57BL/6N ES cells by aggregation with standard albino outbred ICR morulas. As detailed in this work, the use of chemically-defined ES cell culture medium (2i) appears to be crucial for the success of the experiment. This is an interesting development that can help the biomedical research community for the easy and rapid generation of C57BL/6N -derived chimeras at a reduced cost. It also complements the toolbox by which chimeras can be obtained with the popular C57BL/6N ES cells, used in the large-scale international consortiums (i.e. IKMC) aiming to generate systematic knock-outs of all genes in the mouse genome.
Tags: chimeras, mouse ES cells, publication
Posted in ES cells, coat color, gene trap, general, genes, information, knock-outs, pigmentation, publication, transgenic research, web site | No Comments »
Monday, June 28th, 2010
SYMPOSIUM :New generation GMOs: Refinement in procedures, Helsinki 2010
The symposium on “New generation GMOs: Refinement in procedures“, chaired by Belén Pintado and Jan Bas Prins, co-sponsored by the International Society forTransgenic Technologies (ISTT), organized within the Scand-FELASA 2010 meeting “New paradigms in laboratory animal science”, was held in Helsinki (Finland), on June 14-17, 2010. The event was a great success and higlighted the importance of “promoting the activities of ISTT among scientific collectives not directly involved in transgenesis, but that have close contact with present or future individuals that may benefit from our society”, as Belen Pintado concluded in her meeting report.
Tags: FELASA, Meeting report, Welfare
Posted in ISTT, Meeting report, Societies, blog, cryopreservation, facility, general, information, meetings, mouse strains, transgenic, transgenic research, web site | No Comments »
Saturday, June 26th, 2010
Mouse chimera generated by injecting E14 ES cells into albino blastocysts (picture by Sergey Kupriyanov)
New images have been added to the ISTT collection of mouse pictures. Our colleague, Sergey Kupriyanov (The Scripps Research Institute, Mouse Genetics Core, La Jolla, CA, USA), has contributed with new pictures of beautiful “white creamy” mouse chimeras, obtained by injecting E14 ES cells into an albino blastocyst. Up to 264 pictures and 60 videos are now available from the ISTT web site, members-only section, illustrating different aspects of the generation and analysis of transgenic animals. This great multimedia resource is most appreciated for teaching and learning purposes.
Tags: chimeras, coat color, collection, pictures
Posted in ES cells, coat color, general, information, knockout, pigmentation, transgenic research, web site | No Comments »
Monday, May 31st, 2010
Genetic polymorphisms among C57BL/6 mouse inbred strains
A manuscript entitled “Genetic polymorphisms among C57BL/6 mouse inbred strains” has been just published by Esther Zurita, Mónica Chagoyen, Marta Cantero, Rosario Alonso, Anna González-Neira, Alejandro López-Jiménez, José Antonio López-Moreno, Carlisle P. Landel, Javier Benítez, Florencio Pazos and Lluís Montoliu in Transgenic Research. The Illumina® Mouse Medium Density Linkage Mapping panel, with 1,449 single nucleotide polymorphisms (SNPs), was used to genotype individuals from ten C57BL/6-related strains: C57BL/6JArc, C57BL/6J from The Jackson Lab, C57BL/6J from Crl, C57BL6/JRccHsd, C57BL/6JOlaHsd, C57BL/6JBomTac, B6(Cg)-Tyr (<c-2j>)/J, C57BL/6NCrl, C57BL/6NHsd and C57BL/6NTac. Twelve SNPs were found informative to discriminate between the “/N” and “/J” C57BL/6 mouse substrains considered. These results will be instrumental for the correct genetic monitoring and appropriate mouse colony handling of different transgenic and knockout mice produced in distinct C57BL/6 inbred substrains. The SNP raw data are also available from the Mouse Phenome Database at Jackson Laboratory.
Tags: C57BL/6 mice, genetic background, mouse inbred strain, polymorphisms, SNP
Posted in ES cells, TT meeting, coat color, database, general, genes, information, knockout, meetings, mouse strains, pigmentation, publication, transgenic, transgenic research, web site | No Comments »
Wednesday, May 5th, 2010
EMMA-JAX cryopreservation course 2010
The Istituto di Biologia Cellulare of Consiglio Nazionale delle Ricerche-”A. Buzzati-Traverso” Campus and The Jackson Laboratory offer a comprehensive course on cryopreservation of mouse embryos, sperm and ovaries. This Course is financially supported by the EU FP7 Capacities Specific Program, through the European Mouse Mutant Archive (EMMA). The course will be held at the “A. Buzzatti-Traverso” Campus, in Monterotondo, Rome, Italy on October 25-29, 2010.
The course is offered to teach methods in cryopreservation for banking of various strains of mice used in research, including inbred, transgenic and knock-out strains. Several methods of cryopreservation are now available and because no single method is adequate for all the various strains of mice being developed, a variety of methods are taught. The course is designed primarily as a “hands-on” laboratory program in which participants learn techniques for the cryopreservation of cleavage-stage embryos, spermatozoa and ovaries.
Additional information about this EMMA-JAX Cryocourse 2010
Application form
Deadline for submitting applications: September 25, 2010
Tags: Cryopreservation course, EMMA, The Jackson Laboratory
Posted in blog, course, cryopreservation, general, information, transgenic research, web site | No Comments »
Thursday, April 29th, 2010
TT2010 meeting report published in Transgenic Research
The scientific report corresponding to the 9th Transgenic Technology meeting (TT2010), recently held in Berlin, Germany, at the MDC, in March 22-24, 2010 has been published, as online first article (DOI 10.1007/s11248-010-9397-2, published online, 24 April 2010), in the scientific journal Transgenic Research, associated to ISTT. This meeting report has been prepared by Thom Saunders (Univ. Michigan, Ann Harbor, MI, USA) and Peter Sobieszczuk (Univ. Miami, FL, USA). Thom and Peter will be the organizers of the next 10th Transgenic Technology meeting (TT2011), to be held in Miami, Florida, on October 24-26, 2011.
Transgenic Research
Tags: Meeting report, TT2010 report
Posted in ISTT Members, Meeting report, TT meeting, general, information, publication, transgenic, transgenic research, web site | No Comments »
Sunday, April 18th, 2010
Scand-FELASA 2010 meeting
The next FELASA (the Federation of Laboratory Animal Science Associations) meeting, a joint FELASA/Scand-LAS symposium, entitled “New paradigms in Laboratory Animal Science” is co-sponsored by the International Society for Transgenic Technologies (ISTT). This combined meeting will be held in Helsinki, Finland, on June 14-17, 2010. The meeting includes a symposium on “New generation GMOs: refinement in procedures“, co-organized by Belén Pintado, current ISTT Treasurer.
Tags: FELASA, ISTT
Posted in ISTT, ISTT Members, Societies, general, information, meetings, transgenic research | No Comments »
