Archive for the ‘Manual’ Category
Tuesday, February 7th, 2012
BIOTERIOS.COM a web portal for Laboratory Animal Science in Spanish
The International Society for Transgenic Technologies (ISTT) recently co-sponsored a mouse meeting in Chile, at the CECs, in Valdivia, organized by Juan Manuel Baamonde (Manager of the CECs Animal Facility) and his colleagues. Juan M. Baamonde is also the creator and the soul behind a most interesting initiative worth knowing: the web portal BIOTERIOS.COM, an impressive web site containing plenty of information about many different aspects of laboratory animals, with texts, protocols, opinions, links to other sites and societies, meant to share information, to provide educational tools, resources and, in general, to trigger a discussion forum on the care and use of animals in research, and all the associated aspects that need to be taken into account, conforming what we usually know as Laboratory Animal Science.
One of the remarkable features of BIOTERIOS.COM is the language used, Spanish, uncommon for this type of initiatives in our field, mostly dominated by resources created in English, for obvious reasons. Therefore the BIOTERIOS.COM web portal is made in Spanish, a language that, according to most estimates, is the second most-spoken language in the world, after the Chinese (Mandarin), and close to English, the third most-used language. Some 400 million people use Spanish as their first language. This fact, along with all the rich and useful contents included, makes BIOTERIOS.COM a reference web site for Latin-American countries and Spain as a useful resource for Laboratory Animal Science in Spanish. Juan M. Baamonde, currently the General Editor of BIOTERIOS.COM, and a most recent member of the ISTT, must be praised for his pioneer idea and for his continuous efforts promoting contents and discussions in this web portal. More than 10% of ISTT Members commonly use or can understand Spanish (including our colleagues from Brazil and Portugal, whose mother language is Portughese). They all can benefit from this web portal, BIOTERIOS.COM, to follow the latest updates on Laboratory Animal Science.
Tags: animal experimentation, animal welfare, ISTT website, laboratory animal science, Latin-American countries, Spanish, web portal
Posted in animal biotechnology, animal experimentation, animal welfare, bioethics, co-sponsorhip, course, general, information, ISTT, ISTT Members, legislation, Manual, membership, practical course, publication, rodent genetics, transgenic research, web site | No Comments »
Wednesday, December 21st, 2011
The transgenic-list (tg-l) has been moved to ISTT
Today, December 21st, 2011, the historical transgenic-list (tg-l), created by Peter Sobieszczuk more than 15 years ago, in July, 1996, has been moved and successfully transferred to its new home at the ISTT web server, following the agreement signed on September 30th, 2011, between Peter and the ISTT. All current subscribers have been transferred automatically and they will be able to continue using and enjoying this useful resource within the field of transgenic animals. This is the new tg-l home web page, where additional information about this transfer and the new features of the tg-l at the ISTT can be browsed. At the ISTT, the tg-l will continue being public (anyone may subscribe, including ISTT members and non-members alike, but subject to email address verification), unmoderated (messages will not be altered by the list administrator) and closed (only subscribers may post messages). All questions, comments, requests, doubts should be addressed to the new tg-l administrators at: tglist_admin@transtechsociety.org.
Tags: ISTT, tg-l, tg-list, transgenic-list
Posted in embryos, ES cells, facility, gene trap, general, genes, information, iPS cells, ISTT, knock-outs, knockout, Manual, meetings, phenotyping, pigmentation, rodent genetics, rules, service, sperm, tg-list, transgenic, transgenic research, web site | No Comments »
Friday, September 9th, 2011
Advanced Protocols for Animal Transgenesis. An ISTT Manual
The International Society for Transgenic Technologies (ISTT) is most pleased to announce the publication of its first book, entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thom Saunders, and published by Springer (2011), in collaboration with the ISTT. This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists, most of them members of the ISTT, who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.
Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement. This book comprises 26 chapters, 109 illlustrations and 669 pages.
Table of contents, chapters of this ISTT Manual:
- 1 Patent and Licensing Issues in Transgenic Technology. Karen S. Canady
- 2 Global Resources: Including Gene Trapped ES Cell. Clones – Is Your Gene Already Knocked Out?. Lluis Montoliu
- 3 Designing Transgenes for Optimal Expression. Eduardo Moltó, Cristina Vicente-García, and Lluis Montoliu
- 4 Gene Targeting Vector Design for Embryonic Stem Cell Modifications. Thomas L. Saunders
- 5 Transgenic Production Benchmarks. Thomas J. Fielder and Lluis Montoliu
- 6 Generation of Transgenic Mice by Pronuclear Microinjection. Katja Becker and Boris Jerchow
- 7 Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, and Ignacio Anegon
- 8 Generation of Transgenic Animals by Use of YACs. Almudena Fernández, Diego Muñoz, and Lluis Montoliu
- 9 BAC Transgenes, DNA Purification, and Transgenic Mouse Production. Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
- 10 Generation of Transgenic Animals with Lentiviral Vectors. Carlos Lois
- 11 Vertebrate Transgenesis by Transposition. Aron Geurts, Darius Balciunas, and Lajos Mates
- 12 Rat Spermatogonial Stem Cell-Mediated Gene Transfer. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, and F. Kent Hamra
- 13 Mouse Cloning by Nuclear Transfer. Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
- 14 Gene Targeting in Embryonic Stem Cells. Elizabeth D. Hughes and Thomas L. Saunders
- 15 The Importance of Mouse ES Cell Line Selection. Wojtek Auerbach and Anna B. Auerbach
- 16 Tetraploid Complementation Assay. Marina Gertsenstein
- 17 Combining ES Cells with Embryos. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, and Marina Gertsenstein
- 18 Derivation of Murine ES Cell Lines. Kristina Nagy and Jennifer Nichols
- 19 Rat Embryonic Stem Cell Derivation and Propagation. Ping Li, Eric N. Schulze, Chang Tong, and Qi-Long Ying
- 20 Induced Pluripotency: Generation of iPS Cells from Mouse Embryonic Fibroblasts. Han Li, Katerina Strati, Verónica Dom?ínguez, Javier Martín, María Blasco, Manuel Serrano, and Sagrario Ortega
- 21 The Preparation and Analysis of DNA for Use in Transgenic Technology. Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
- 22 Colony Management. Karen Brennan
- 23 Cryopreservation. Belén Pintado and Juan de Dios Hourcade
- 24 Shipment of Mice and Embryos. Shirley Pease
- 25 Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy. Jorge M. Sztein, R.J. Kastenmayer, and K.A. Perdue
- 26 Refinement, Reduction, and Replacement. Jan Parker-Thornburg
ISTT Members are entitled to purchase this ISTT Manual with a 33% discount, through the members-only area of the web site.
Posted in animal biotechnology, animal experimentation, animal welfare, coat color, embryos, ES cells, facility, gene trap, general, information, iPS cells, ISTT, ISTT Members, Manual, publication, Springer, transgenic, transgenic research, web site | No Comments »
Tuesday, May 24th, 2011
ISTT Members entitled to purchase Springer books with a 33% discount
The International Society for Transgenic Technologies (ISTT) is most pleased to publicly announce the renewal of the agreement with the publisher Springer, for mutual cooperation, for an additional period of five years. As part of this agreement, ISTT members will continue benefitting from a privileged access to full online contents of the scientific journal Transgenic Research, to which ISTT is associated, through the members-only area of the ISTT web site. As a new benefit for ISTT members, this current agreement includes a list of books on animal transgenesis and animal genetics, published by Springer, which ISTT members are entitled to purchase with a 33% discount, also through the members-only area of the ISTT web site. Of course, these books by Springer include the forthcoming title on “Advanced Protocols for Animal Transgenesis. An ISTT Manual” edited by Shirley Pease and Thom Saunders, promoted by the ISTT and due to be published in August 2011.
Tags: animal experimentation, ISTT Members, publication, Springer, Transgenesis, transgenic research
Posted in animal biotechnology, co-sponsorhip, general, information, ISTT, ISTT Members, Manual, publication, Springer, transgenic research, web site | No Comments »
Friday, May 13th, 2011
IVF and cryopreservation movies from CARD (Japan)
Members of Prof. Naomi Nakagata‘s laboratory, at the Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan, have uploaded various very interesting videos, publicly available from YouTube, illustrating several methods and techniques related to in vitro fertilization (IVF) and the cryopreservation of mouse lines, through sperm and embryos.
These are some of these video files uploaded in YouTube by CARD:
Tags: animal experimentation, cryopreservation, ISTT videos, videos
Posted in animal biotechnology, animal experimentation, cryopreservation, general, information, Manual, video, web site | 1 Comment »
Saturday, April 9th, 2011
Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)
The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.
This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.
Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Table of contents:
- Karen S. Canady: Patent and licensing issues in transgenic technology.
- Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
- Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
- Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
- Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
- Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
- Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
- Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
- Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
- Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
- Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
- Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
- Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
- Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
- Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
- Marina Gertsenstein: Tetraploid Complementation Assay.
- Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
- Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
- Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
- Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
- Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
- Karen Brennan: Colony Management.
- Belen Pintado and Juan Hourcade: Cryopreservation.
- Shirley Pease: Shipment of Mice and Embryos.
- Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
- Jan Parker-Thornburg: Refinement, Reduction and Replacement
ISTT Members are entitled to a 33% discount on the book price.
Tags: animal experimentation, animal welfare, ISTT, ISTT Manual, ISTT Members, methods, mouse ES cells, Springer, Transgenesis, transgenic mice, transgenic research
Posted in animal biotechnology, animal welfare, coat color, cryopreservation, ES cells, facility, gene trap, general, genes, information, iPS cells, ISTT, ISTT Members, knock-outs, knockout, legislation, Manual, mouse strains, publication, rat, rodent genetics, transgenic research, web site | No Comments »
