Ana Isabel Nieto (Granada, Spain) is the winner of a copy of the ISTT Manual:Advanced Protocols for Animal Transgenesis (edited by Shirley Pease and Thomas Saunders, published by Springer, 2011) from bioterios.com, the most popular South-American web portal for Laboratory Animal Science, in Spanish, organized and led by the ISTT member Juan Manuel Baamonde (CECs, Valdivia, Chile). The International Society for Trangenic Technologies (ISTT) and bioterios.com are mutually supporting and promoting their activities. The web portal bioterios.com has been referred previously from this ISTT blog, and also the launching of their new web layout, earlier this year. Likewise, the ISTT and the TT2014 meeting have also been promoted from bioterios.com.
Archive for the ‘Manual’ Category
This past week, 7-11 October 2013, the CARD-CNB Mouse Sperm and Embryo Cryopreservation Course was held at CNB-CSIC, in Madrid, Spain, with great success and accompanied with also great sunny weather. This was the first cryopreservation course of this kind, co-organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain), were the newest methods developed by CARD, at the Nakagata lab, were demonstrated in Europe, directly by the CARD team. The instructors at this CARD-CNB course were commanded by the CARD-University of Kumamoto team, from Japan (Toru Takeo, Kiyoko Fukumoto, Tomoko Kondo, Yukie Haruguchi, Yumi Takeshita, Yuko Nakamuta and Shuuji Tsuchiyama), and additional help and collaboration was provided from the Mouse Biology Program at UC-Davis, CA, USA (Kristy Kinchen), from INIA, Madrid, Spain (Raúl Fernández), from CIEMAT, Madrid, Spain (Jesús Martínez), from USA (Jorge Sztein), from Paratechs, Lexington, KY, USA (Barbara Stone) and from CNB-CSIC (Julia Fernández, María Jesús del Hierro, Marta Castrillo and Isabel Martín-Dorado), for several of the methods demonstrated. All instructors must to be praised for their deep knowledge, patience and extraordinary dedication and commitment towards the success of this course. Complementary and most interesting lectures were provided on a wide variety of topics related to the course main focus, including: animal welfare and regulations by Belén Pintado and Jorge Guillén; the history, fundaments and comparison of methods by Jorge Sztein; the effects of the in vitro culture of mouse embryos by Alfonso Gutiérrez-Adán; safety and handling issues of liquid nitrogen by Jesús Martínez; shipping frozen and refrigerated materials by Toru Takeo, databases in a cryopreservation lab, by Shuuji Tsuchiyama, about EMMA by Lluis Montoliu and CARD by Naomi Nakagata, as examples of mouse embryo and sperm archives, and, also, on the new editing nucleases for genome modification, by Kai Schönig (Mannheim, Germany), a talk sponsored by Sigma.
As many as 24 participants, coming from research institutions or companies located in 16 countries around the world (UK, Spain, Australia, USA, Canada, Czech Republic, Brazil, Finland, France, Denmark, Israel, Netherlands, Portugal, Sweden, Italy and Taiwan) were presented with the latest advances in mouse sperm and embryo cryopreservation and all associated mouse reproductive biology ancillary techniques.
The topics covered by the course included the following major areas: obtaining sperm from mouse cauda epididymis, obtaining unfertilized mouse oocytes, three different types of in vitro fertilization techniques (using fresh, frozen or refrigerated sperm), vitrification of unfertilized oocytes and 2-cell embryos (freezing and thawing), slow-method for freezing and thawing 2-cell embryos, refrigerated sperm and 2-cell embryos, abdominal and scrotal vasectomies, three types of embryo transfer (oviduct, uterus and non-surgical, with NSET tools), freezing and thawing mouse sperm and ICSI, among many additional common methodologies used to handle mouse embryos and gametes adequately.
The course was very intensive, but the kind atmosphere created by participants and instructors was excellent and, hence, all the tight and carefully devised demonstrations and practices, packed within a very busy schedule, could be run smoothly and successfully without problems. The vast experience in running this type of cryopreservation courses and the remarkable professionality of our colleagues from CARD-University of Kumamoto were key for the accomplished success. All methods followed their three-step learning process. At first, the theory and fundaments were briefly provided and summarized. Then, the method was demonstrated by instructors and, finally, the participants executed the procedures by themselves, with the help of instructors.
The participants left this cryopreservation course to return to their countries and institutions with the most satisfactory results obtained and with plenty of new information to digest, process and reproduce. All participants were given the task to spread the word and disseminate the use of these highly efficient and robust cryopreservation techniques that have boosted the field.
This CARDCNB cryopreservation course was sponsored by the International Society for Transgenic Technologies (ISTT) and received the co-sponsorship and support from a number of companies whose contributions need to be greatly acknowledged as well: Leica, Charles River, Sigma, Labotect, Cosmo-Bio, Kyudo, Harlan and Paratechs.
The International Society for Transgenic Technologies (ISTT) recently co-sponsored a mouse meeting in Chile, at the CECs, in Valdivia, organized by Juan Manuel Baamonde (Manager of the CECs Animal Facility) and his colleagues. Juan M. Baamonde is also the creator and the soul behind a most interesting initiative worth knowing: the web portal BIOTERIOS.COM, an impressive web site containing plenty of information about many different aspects of laboratory animals, with texts, protocols, opinions, links to other sites and societies, meant to share information, to provide educational tools, resources and, in general, to trigger a discussion forum on the care and use of animals in research, and all the associated aspects that need to be taken into account, conforming what we usually know as Laboratory Animal Science.
One of the remarkable features of BIOTERIOS.COM is the language used, Spanish, uncommon for this type of initiatives in our field, mostly dominated by resources created in English, for obvious reasons. Therefore the BIOTERIOS.COM web portal is made in Spanish, a language that, according to most estimates, is the second most-spoken language in the world, after the Chinese (Mandarin), and close to English, the third most-used language. Some 400 million people use Spanish as their first language. This fact, along with all the rich and useful contents included, makes BIOTERIOS.COM a reference web site for Latin-American countries and Spain as a useful resource for Laboratory Animal Science in Spanish. Juan M. Baamonde, currently the General Editor of BIOTERIOS.COM, and a most recent member of the ISTT, must be praised for his pioneer idea and for his continuous efforts promoting contents and discussions in this web portal. More than 10% of ISTT Members commonly use or can understand Spanish (including our colleagues from Brazil and Portugal, whose mother language is Portughese). They all can benefit from this web portal, BIOTERIOS.COM, to follow the latest updates on Laboratory Animal Science.
Today, December 21st, 2011, the historical transgenic-list (tg-l), created by Peter Sobieszczuk more than 15 years ago, in July, 1996, has been moved and successfully transferred to its new home at the ISTT web server, following the agreement signed on September 30th, 2011, between Peter and the ISTT. All current subscribers have been transferred automatically and they will be able to continue using and enjoying this useful resource within the field of transgenic animals. This is the new tg-l home web page, where additional information about this transfer and the new features of the tg-l at the ISTT can be browsed. At the ISTT, the tg-l will continue being public (anyone may subscribe, including ISTT members and non-members alike, but subject to email address verification), unmoderated (messages will not be altered by the list administrator) and closed (only subscribers may post messages). All questions, comments, requests, doubts should be addressed to the new tg-l administrators at: firstname.lastname@example.org.
The International Society for Transgenic Technologies (ISTT) is most pleased to announce the publication of its first book, entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thom Saunders, and published by Springer (2011), in collaboration with the ISTT. This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists, most of them members of the ISTT, who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.
Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement. This book comprises 26 chapters, 109 illlustrations and 669 pages.
Table of contents, chapters of this ISTT Manual:
- 1 Patent and Licensing Issues in Transgenic Technology. Karen S. Canady
- 2 Global Resources: Including Gene Trapped ES Cell. Clones – Is Your Gene Already Knocked Out?. Lluis Montoliu
- 3 Designing Transgenes for Optimal Expression. Eduardo Moltó, Cristina Vicente-García, and Lluis Montoliu
- 4 Gene Targeting Vector Design for Embryonic Stem Cell Modifications. Thomas L. Saunders
- 5 Transgenic Production Benchmarks. Thomas J. Fielder and Lluis Montoliu
- 6 Generation of Transgenic Mice by Pronuclear Microinjection. Katja Becker and Boris Jerchow
- 7 Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, and Ignacio Anegon
- 8 Generation of Transgenic Animals by Use of YACs. Almudena Fernández, Diego Muñoz, and Lluis Montoliu
- 9 BAC Transgenes, DNA Purification, and Transgenic Mouse Production. Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
- 10 Generation of Transgenic Animals with Lentiviral Vectors. Carlos Lois
- 11 Vertebrate Transgenesis by Transposition. Aron Geurts, Darius Balciunas, and Lajos Mates
- 12 Rat Spermatogonial Stem Cell-Mediated Gene Transfer. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, and F. Kent Hamra
- 13 Mouse Cloning by Nuclear Transfer. Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
- 14 Gene Targeting in Embryonic Stem Cells. Elizabeth D. Hughes and Thomas L. Saunders
- 15 The Importance of Mouse ES Cell Line Selection. Wojtek Auerbach and Anna B. Auerbach
- 16 Tetraploid Complementation Assay. Marina Gertsenstein
- 17 Combining ES Cells with Embryos. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, and Marina Gertsenstein
- 18 Derivation of Murine ES Cell Lines. Kristina Nagy and Jennifer Nichols
- 19 Rat Embryonic Stem Cell Derivation and Propagation. Ping Li, Eric N. Schulze, Chang Tong, and Qi-Long Ying
- 20 Induced Pluripotency: Generation of iPS Cells from Mouse Embryonic Fibroblasts. Han Li, Katerina Strati, Verónica Dom?ínguez, Javier Martín, María Blasco, Manuel Serrano, and Sagrario Ortega
- 21 The Preparation and Analysis of DNA for Use in Transgenic Technology. Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
- 22 Colony Management. Karen Brennan
- 23 Cryopreservation. Belén Pintado and Juan de Dios Hourcade
- 24 Shipment of Mice and Embryos. Shirley Pease
- 25 Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy. Jorge M. Sztein, R.J. Kastenmayer, and K.A. Perdue
- 26 Refinement, Reduction, and Replacement. Jan Parker-Thornburg
The International Society for Transgenic Technologies (ISTT) is most pleased to publicly announce the renewal of the agreement with the publisher Springer, for mutual cooperation, for an additional period of five years. As part of this agreement, ISTT members will continue benefitting from a privileged access to full online contents of the scientific journal Transgenic Research, to which ISTT is associated, through the members-only area of the ISTT web site. As a new benefit for ISTT members, this current agreement includes a list of books on animal transgenesis and animal genetics, published by Springer, which ISTT members are entitled to purchase with a 33% discount, also through the members-only area of the ISTT web site. Of course, these books by Springer include the forthcoming title on “Advanced Protocols for Animal Transgenesis. An ISTT Manual” edited by Shirley Pease and Thom Saunders, promoted by the ISTT and due to be published in August 2011.
Members of Prof. Naomi Nakagata‘s laboratory, at the Division of Reproductive Engineering, Center for Animal Resources & Development (CARD), Kumamoto University, Kumamoto, Japan, have uploaded various very interesting videos, publicly available from YouTube, illustrating several methods and techniques related to in vitro fertilization (IVF) and the cryopreservation of mouse lines, through sperm and embryos.
These are some of these video files uploaded in YouTube by CARD:
- Collection of Oocytes
- remove-ce [remove cauda epididymides]
- Preparation of sperm suspension
- Insemination by frozen-thawed sperm (IVF)
- Warm straw which includes frozen sperm suspension of mice
- Transferring thawed sperm suspension into a drop of FERTIUP
- Float straws including mice sperm suspension and freeze it
- Thawing of vitrified mice embryos
- Thawing of vitrified mice embryos-recommemded speed of pipetting-
- Dryshipper (in Japanese)
The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.
This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.
Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.
Table of contents:
- Karen S. Canady: Patent and licensing issues in transgenic technology.
- Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
- Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
- Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
- Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
- Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
- Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
- Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
- Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
- Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
- Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
- Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
- Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
- Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
- Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
- Marina Gertsenstein: Tetraploid Complementation Assay.
- Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
- Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
- Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
- Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
- Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
- Karen Brennan: Colony Management.
- Belen Pintado and Juan Hourcade: Cryopreservation.
- Shirley Pease: Shipment of Mice and Embryos.
- Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
- Jan Parker-Thornburg: Refinement, Reduction and Replacement