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CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

Friday, February 22nd, 2013
CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course, Madrid, Spain, 7-11 October 2013

The International Society for Transgenic Technologies (ISTT) has agreed to co-sponsor the CARD-CNB Mouse Sperm and Embryo Cryopreservation Practical Course that will be held at the National Centre for Biotechnology (CNB-CSIC), in Madrid, CSIC, during one week, on 7-11 October 2013, organized by Naomi Nakagata (CARD-Kumamoto University, Japan, Coordinator of CARD) and Lluis Montoliu (CNB-CSIC, Madrid, Spain, Coordinator of the Spanish EMMA node).

This course is open to anyone interested. Pre-application will be required, including, at least, a recent CV and a letter prepared by the intended participant describing how the applicant will benefit by attending this course and how relevant is the course material to his/her work. Additional documents are welcome, at the discretion of participants, including supporting letters by supervisors (where appropriate), reference letters, etc… Pre-applications should be submitted by email to cryocourse@cnb.csic.es by 31 May 2013.

The maximum number of participants attending this course will be 20, distributed among countries and institutions, and according the documentation provided and the interests expressed. Pre-applications will be accepted until 31 May 2013. The review and selection of participants will be done by the Organizers from 1 to 15 June 2013. Registrations and payments for selected participants will be accepted from June 16, 2013 to August 31, 2013. If required, an ordered waiting list will be prepared and any cancellation or unpaid registration by 31 August 2013 will be readily substituted by the first available person from this waiting list, starting on 1 September 2013.

The course registration fee is 800 Euros (with a reduced fee of 750 Euros for ISTT Members). This fee includes participation in the entire course, all materials and reagents, lunches over the 5 days and one course official dinner. Hotel costs are not included in the registration fee but booking assistance will be provided, if required, at a convenient nearby hotel, close to CNB Campus, where all instructors and lecturers will be also lodged, hence further promoting interaction from breakfast to dinner. The official language of the course will be English.

COURSE INFORMATION: Recent developments from the laboratory of Prof. Naomi Nakagata (CARD-Kumamoto University, Japan) have boosted the mouse cryopreservation field with improved methods for fresh and frozen sperm techniques and associated optimized IVF methods that have resulted in unparalleled increased efficiencies for the cryopreservation and rescue of relevant mouse lines. At the CNB-CSIC in Madrid, hosting the Spanish EMMA node, these new CARD cryopreservation methods have been successfully implemented and, moreover, a fruitful collaboration has been established with the laboratory of Prof. Naomi Nakagata, eventually resulting in the signature of an institutional cooperation agreement between the CSIC and the University of Kumamoto, under the framework of which this cryopreservation course is organized. The aim of this course is to introduce the new CARD methods to researchers and technicians involved in managing mouse archiving and/or transgenic facilities and willing to implement these new methods, directly taught by the team which devised them. Each participant will have one stereomicroscope and the entire set of tools, reagents and animals required to learn and practice all the methods included in the program of this course. In addition to practical sessions, the course will also include several lectures of related interesting topics for the participants delivered by experts in each field. The number of instructors and lecturers appointed is 20.

Hands-on topics that will be covered during this cryopreservation course

  • making pipettes and practising embryo handling
  • isolating unfertilized mouse oocytes
  • isolating and cold storage/shipping of mouse cauda epididymis
  • freezing/thawing mouse sperm and IVF
  • fresh mouse sperm and IVF
  • freezing/thawing 2-cell IVF-derived mouse embryos
  • vitrification of mouse oocytes and embryos
  • embryo transfer techniques in mice
  • vasectomy of male mice
  • demonstration of ICSI

Additional lectures on the following topics

  • new EU Directive on the protection of animals for experimentation
  • new US Guidelines for the use of animals in research
  • epigenetic effects of in vitro culture on mouse embryos
  • databases for handling information in cryopreservation facilities
  • shipping mice, refrigerated and frozen material
  • safety issues handling liquid nitrogen in a cryopreservation facility
  • EMMA and Infrafrontier-I3
  • MMRRC

Instructors

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Kiyoko Fukumoto (CARD-Kumamoto University, Japan)
  • Tomoko Kondo (CARD-Kumamoto University, Japan)
  • Yukie Haruguchi (CARD-Kumamoto University, Japan)
  • Yumi Takeshita (CARD-Kumamoto University, Japan)
  • Yuko Nakamuta (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Raul Fernández (INIA, Madrid, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)
  • Julia Fernández (CNB-CSIC, Madrid, Spain)
  • María Jesús del Hierro (CNB-CSIC, Madrid, Spain)
  • Marta Castrillo (CNB-CSIC, Madrid, Spain)
  • Isabel Martín-Dorado (CNB-CSIC, Madrid, Spain)
  • Kristy Kinchen (Mouse Biology Program, UC Davis, CA, USA)

Additional lectures by

  • Naomi Nakagata (CARD-Kumamoto University, Japan)
  • Toru Takeo (CARD-Kumamoto University, Japan)
  • Shuji Tsuchiyama (CARD-Kumamoto University, Japan)
  • Alfonso Gutiérrez-Adán (INIA, Madrid, Spain)
  • Kent Lloyd (Mouse Biology Program, UC Davis, CA, USA)
  • Belén Pintado (CNB-CSIC, Madrid, Spain)
  • Jesús Martínez Palacio (CIEMAT, Madrid, Spain)
  • Javier Guillén (AAALAC, Pamplona, Spain)
  • Lluis Montoliu (CNB-CSIC, Madrid, Spain)

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Posted in animal experimentation, bioethics, co-sponsorhip, embryos, ISTT Members, practical course, rodent genetics, rules, sperm, web site, workshop | No Comments »

Douglas Coleman and Jeffrey Friedman granted the 2012 BBVA Foundation Frontiers of Knowledge Award in Biomedicine

Tuesday, January 29th, 2013
Douglas Coleman and Jeffrey Friedman granted the 2012 BBVA Foundation Frontiers of Knowledge Award in Biomedicine

Douglas Coleman and Jeffrey Friedman granted the 2012 BBVA Foundation Frontiers of Knowledge Award in Biomedicine

The 2012 BBVA Foundation Frontiers of Knowledge Award in Biomedicine has been granted to Douglas Coleman (retired, Professor Emeritus at The Jackson Laboratory, Bar Harbor, ME, USA) and Jeffrey Friedman (The Rockfeller University, USA) for “revealing the existence of the genes involved in the regulation of appetite and body weight, a discovery crucial to our understanding of human pathologies such as obesity,” as stated by the prize jury.

Jeffrey Friedman cloned the gene encoding leptin (Lep), as the mutation present in the obese mice (ob/ob), based on the ideas and previous work pioneered by Douglas Coleman, who also predicted that the mutant (db/db) mice affected a gene (Lepr) encoding the receptor for this important hormone, produced by adipose cells in the fat and acting in the brain to regulate food intake, energy expenditure and how much fat the body stores. This is yet another award and public recognition to mouse genetics, leading the discovery of genes and their functions, and illustrating how mice help to find out what the homologous human loci do, and their relevance in pathology when they are mutated.

Douglas Coleman and Jeffrey Friedman have been already granted the Shaw Prize in Life Science and Medicine, in 2009, and the Albert Lasker Basic Medical Research Award, in 2010, also jointly in both cases, for their discovery of leptin. Both scientists should be now congratulated, once again, for their excellent scientific work and this new award, truly deserved. Anyone interested to read about the scientific histories behind the discovery of leptin is welcome to read the following articles:

Leading the charge in leptin research: an interview with Jeffrey Friedman.
Friedman J. Dis Model Mech. 2012 Sep;5(5):576-9.

A historical perspective on leptin.
Coleman DL. Nat Med. 2010 Oct;16(10):1097-9.

The 2012 Jury for this BBVA Foundation Frontiers of Knowledge Award in Biomedicine comprises a number of eminent scientists including two researchers that are, in addition, ISTT members, namely: Robin Lovell-Badge, Head of the Division of Stem Cell Biology and Developmental Genetics at the National Institute for Medical Research (Medical Research Council, UK), acting as the Secretary of this Jury; and Bruce Whitelaw, Head of the Developmental Biology Division at The Roslin Institute in Edinburgh (UK) and Editor-in-Chief of Transgenic Research, the scientific journal to which the International Society for Transgenic Technologies (ISTT) is associated.

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Posted in genes, information, Lasker Awards, rodent genetics, web site | No Comments »

The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

Sunday, September 23rd, 2012
The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The ISTT journey: from Barcelona to Guangzhou, now it is time for China! The TT2013 meeting

The International Society for Transgenic Technologies (ISTT) was founded shortly after the Transgenic Technology (TT) meeting in Barcelona (TT2005). Since then, the ISTT family has been fortunate to visit several countries, every ~18 months. In 2007 we went to Brisbane (TT2007).  In 2008, we visited Toronto (TT2008). In 2010 we returned to Europe and held the TT2010 meeting in Berlin. For 2011 we visited the USA for the first time, and organized the TT2011 meeting in St Pete Beach (Florida). And, now, the next TT meeting (TT2013) is planned for China, in Guangzhou. This has been one of our aims and challenges, since the foundation of the ISTT, namely, holding a TT meeting in Asia, in China. Now it has become a reality. The 11th Transgenic Technology meeting (TT2013) will be held in Guangzhou (China), on 25-27 February 2013, organized by Prof. Ming Zhao (Chair) (Southern Medical University, Guangzhou) and his Organizing and Advisory Committees, immediately after celebrating the Chinese New Year of the Snake. More than 30 speakers have confirmed their participation, to discuss about ES cells, iPS cells, targeted nucleases (ZFNs and TALENs), cryopreservation and reproduction techniques, running a transgenic facility, mouse genetics, epigenetics, ethics and animal welfare, transgenesis in other vertebrates, animal models of disease, etc… among many other interesting topics. At the TT2013 meeting, the ISTT will award the 9th ISTT Prize for outstanding contributions to transgenic technologies to Prof. Allan Bradley,  Director Emeritus of the Wellcome Trust Sanger Institute (WTSI), in Hinxton (UK), and leader of the Mouse Genomics Team at WTSI.

In addition, a 3-day hands-on practical workshop (28 February-2 March 2013) will be offered in Guangzhou after the TT2013 meeting, addressing basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management and other interesting topics. This workshop is organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair,  Ming Zhao (Southern Medical University, Guangzhou, China), Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China).

Don’t miss this opportunity to hear the latest advances in animal transgenic by world experts! Time is going fast and first deadlines (15 October 2012), for registering at reduced fees, for submitting abstracts, for applying for ISTT registration awards and for nominating candidates for ISTT Young Investigator awards are rapidly approaching.

See you all in China!

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Posted in animal welfare, bioethics, course, cryopreservation, embryos, ES cells, facility, iPS cells, ISTT, ISTT Members, ISTT Prize, knock-outs, meetings, practical course, registration awards, rodent genetics, sperm, transgenic research, travel awards, TT meeting, web site, workshop, Young Investigator Award | No Comments »

EMBL Course: Laboratory Animal Science, 8-19 April 2013, Monterotondo, Italy

Thursday, July 26th, 2012
EMBL Course: Laboratory Animal Science, 8-19 April 2013, Monterotondo, Italy

EMBL Course: Laboratory Animal Science, 8-19 April 2013, Monterotondo, Italy

A two-week intensive course on laboratory animal science will be organized at the EMBL Monterotondo, in April 2013, run by Dr. Maria Kamber, who has organized 5 successful courses in Greece at the BSRC Alexander Fleming. The objective of this course is to present basic facts and principles that are essential for the humane use and care of animals and for the quality of research. The contents of the course are in line with recommendations of the Federation of European Laboratory Animal Science Associations (FELASA) regarding the training of the young scientists whose research involves the use of vertebrate animals.

This prestigious event is addressed to professionals from fields of Biomedical Science. Past participants include biologists, veterinarians, medical practitioners and pharmacologists from all across Europe, Asia and Africa. This is a revolutionary course within the scientific community as it awakes a new wave of consciousness regarding animal welfare and quality research. The vision is to train a new breed of scientists with acute awareness of what humane research actually means. It breaks with tradition in the fact that it trains participants not only in the passive acquisition of knowledge, but in the active process of critical thinking. The fundamental principle the course aims to promote is that the experimental outcome of research strongly depends on the humane treatment of the laboratory animals.

Upon the completion each participant is able to understand and perform humane use and care of laboratory animals, and clearly comprehend the vital principles for quality research. Successful participants are awarded a certificate, now a requirement according to the revised European Directive, which demonstrates competence in working with laboratory animals for research purposes.

This course is under evaluation for accreditation from FELASA Board.

WEB site of the course Application deadline: Friday 1 March 2013

Registration will open soon. If you would like to be update, please subscribe to LAS2013@embl.it .

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Posted in animal biotechnology, animal experimentation, animal welfare, bioethics, course, general, information, legislation, practical course, rodent genetics, web site | No Comments »

One day meeting in London on Conditional Transgenic Techniques: Principles & Best Practice

Tuesday, July 24th, 2012
One day meeting in London on Conditional Transgenic Techniques: Principles & Best Practice

One day meeting in London on Conditional Transgenic Techniques: Principles & Best Practice

Registration is now open for a new transgenic training event on “Conditional Transgenic Techniques: Principles & Best Practice“, organized by the RSPCA, Wellcome Trust Sanger Institute, the Leeds Institute of Molecular Medicine, the Mary Lyon Centre from MRC in Harwell, and the National Institute for Medical Research from MRC in London.

This is a one day meeting on current best practice in the production of genetically altered mice, using conditional transgenic techniques. Topics will include:
* the technology of inducible and conditional systems
* examples of their use
* recombinases: whats available, limitations & how to test efficacy
* conditional allele resources
* breeding schemes

The meeting is being held in central London on Thursday 4th October 2012, and is limited to 100 places allocated on a first come first served basis. Registration (including lunch) will cost £60, so for more information or to book a place and get a registration form email GA@rspca.org.uk putting “conditional workshop” in the title field.

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Posted in animal experimentation, course, information, knock-outs, knockout, meetings, rodent genetics, transgenic research, workshop | No Comments »

Registration for the TT2013 meeting in China is OPEN

Thursday, June 7th, 2012
Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

Registration for the TT2013 meeting in China is OPEN (http://www.tt2013.org)

On behalf of the Organizing Committee for the 11th Transgenic Technology (TT2013) meeting and of the International Society for Transgenic Technologies (ISTT) it is my great pleasure to announce the launching of the official TT2013 meeting web site and, at the same time, inform that registration for the TT2013 meeting is now OPEN. After a very successful TT2011 meeting in Florida, the ISTT family travels to far-East, to China, to hold the 11th Transgenic Technology meeting in the city of Guangzhou, the largest city of the Guandong province in Southern China, on February 25-27, 2013. The TT2013 meeting is locally organized by the Southern Medical University, in Guangzhou, and the Chair of the Organizing Committee is Prof. Ming Zhao.

TT2013 meeting web site: http://www.tt2013.org

TT2013 meeting official email address: tt2013@transtechsociety.org

We welcome all attendees who are in the process of generating genetically-modified animals or who perform the experiments that follow to determine the corresponding phenotypes of such transgenic animals. The program should be of interest to scientists, group leaders, postdoctoral researchers, facility managers, technicians and PhD students working directly or indirectly with genetically-modified animals. We invite you to participate and contribute to this conference where we will discuss the latest technology developments, the newest applications and strategies in biology, biomedicine and biotechnology using transgenic animals. Companies and institutions with an interest in this field are kindly invited to participate and share their latest technologies. We encourage you to submit abstracts to be presented at the TT2013 meeting.

We have confirmed a wonderful list of excellent speakers and interesting topics for the animal transgenesis community. The list of invited speakers confirmed to attend the TT2013 meeting includes:

  • Fernando Benavides (MD Anderson Cancer Center, Smithville, TX, USA)
  • Allan Bradley (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK) ISTT Prize
  • James Bussell (Wellcome Trust Sanger Institute, Hinxton/Cambridge, UK)
  • Shannon Byers (The Jackson Laboratory, Bar Harbor, ME, USA)
  • Toni Cathomen (University Medical Center Freiburg, Germany)
  • Alan Colman (Institute of Medical Biology, Singapore) Closing Talk
  • Michael Dobbie (Australian Phenomics Facility, The Australian National University, Canberra, Australia)
  • Scott Fahrenkrug (Recombinetics, Minneapolis, MN, USA)
  • Malcolm France (Sydney University, Sydney, Australia)
  • Xiang Gao (Model Animal Research Center of Nanjing University, Nanjing, PR China)
  • Alfonso Gutiérrez-Adán (Dep. Animal Reproduction, INIA, Madrid, Spain)
  • Yann Herault (Institut Clinique de la Souris, ICS and IGBMC, Illkirch/Strasbourg, France)
  • Benoît Kanzler (Max-Planck Institute of Immunobiology and Epigenetics, Freiburg, Germany)
  • Dietmar Kappes (Fox Chase Cancer Center, Philadelphia, PA, USA)
  • Takashi Kohda (Department of Epigenetics, Medical Research Institute, Tokyo Medical and Dental University, Japan)
  • Thomas Kolbe (Biomodels Austria and Institute for Biotechnology in Animal Production, IFA-Tulln, Austria)
  • Takashi Kuramoto (Institute of Laboratory Animals, Kyoto University, Kyoto, Japan)
  • Liangxue Lai (Guangzhou Institute of Biomedicine and Health, Chinese Academy of Science, Guangzhou, PR China)
  • Jinsong Li (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Ning Li (State Key Laboratories for Agrobiotechnology, China Agricultural University, Beijing, PR China)
  • Kent Lloyd (University of California, Davis, CA, USA)
  • Shoukhrat Mitalipov (Oregon National Primate Research Center, OHSU, Beaverton, OR, USA)
  • Naomi Nakagata (Center for Animal Resources and Development, Kumamoto University, Japan)
  • Catheryn O’Brien (The Walter and Eliza Hall Institute, Melbourne, Australia)
  • Masaru Okabe (Genome Information Research Center Research, Institute for Microbial Diseases, Osaka University, Osaka, Japan)
  • Jan Parker-Thornburg (MD Anderson Cancer Center, Houston, TX, USA)
  • Xin-an Pu (The Ohio State University, Comprehensive Cancer Center, Columbus, OH, USA)
  • Ling Sun (Institute of Developmental Biology and Molecular Medicine, Fudan University, Shanghai, PR China)
  • Davor Solter (Institute of Medical Biology, Singapore) Opening Talk
  • Zhu-Gang Wang (Shanghai Research Center for Model Organisms, Shanghai, PR China)
  • Guoliang Xu (Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, Shanghai, PR China)
  • Bo Zhang (College of Life Sciences, Peking University, Beijing, PR China)
  • Qi Zhou (The State Key Laboratory of Reproductive Biology, Institute of Zoology, Chinese Academy of Sciences, Beijing, PR China)

The list of topics that will be covered at the TT2013 meeting includes the following themes:

  • Androgenetic haploid embryonic stem cells
  • Sperm Cryopreservation and IVF
  • KOMP ES cell clones performance
  • Australian Phenomics Network Initiatives
  • Epigenetics and Transgenesis
  • Effects of in vitro culture on mammalian embryos
  • Epigenetic alterations during mouse transgenesis and cloning
  • Epigenetic reprogramming by oocytes
  • Targeted nucleases, ZFNs and TALENs, in transgenesis
  • Livestock Genome Editing with TALENS
  • Transgenic mice and ZFNs
  • KO pigs and ZFNs
  • Zebrafish and TALENs
  • Transgenic livestock
  • Ethics, animal welfare and regulations
  • Health monitoring protocols and transgenic facilities
  • Animal welfare and international mouse consortia
  • Impact of genetic background in mouse and rat models
  • Assisted reproduction strategies in mice
  • Non-rodent transgenesis
  • Primate chimeras and ES cells
  • Rat functional genomic initiatives
  • National BioResource Project for the rat
  • Animal models of human diseases
  • ES and iPS cells
  • transposon (PiggyBac)-mediated mutagenesis
  • Round-table discussion: Running a transgenic facility
  • Running a transgenic facility: The business aspect of running a transgenic unit
  • Running a transgenic facility: managing errors, difficulties
  • Running a transgenic facility: Staying on the cutting edge of technology while still maintaining basic services
  • Rat/mouse chimeras and iPS/ES cells
  • Overview of cre-transgenic mouse lines resources
  • China/Asia/Oceania in the International Mouse Functional Genomics Consortia
  • The Shanghai Research Center for Model Organisms

The TT2013 meeting will be held at the Baiyun International Convention Center-Oriental International Convention Hotel in Guangzhou, the largest comprehensive five-star hotel in Southern China, with an established record of hosting successful meetings and perfect supporting facilities. Located on the Baiyun Mountain, in the Baiyun New Town and overlooking the Baiyun Mountain Scenic Area and the Oriental Resort, this Convention Center is a fully equipped, self-contained meeting resort. More than 40 international air routes serve the new Baiyun International Airport, a 20 minute drive from the Baiyun International Convention Center.

After the TT2013 meeting, there will be a 3-days hands-on practical workshop on transgenic techniques, organized by Wenhao Xu (University of Virginia, Charlottesville, VA, USA), Chair; Ming Zhao (Southern Medical University, Guangzhou, China); Jing An (Cancer Institute, Southern Medical University, Guangzhou, China) and Liangping Li (Sun Yat-sen University, Guangzhou, China). This workshop is limited to 30 participants. Instructors for the practical course will include: Wenhao Xu (USA), Xin-an Pu (USA), Anna Auerbach (USA), Lily Reed (USA), Xin Rairdan (USA), Barbara Stone (USA), Steven Xing (USA), Margaret Landis (USA), Katja Becker (Germany), Ronald Naumann (Germany). Techniques that will be discussed and demonstrated at this course include: basic microinjection techniques, piezo injection, laser-assisted application, non-surgical implantation, mouse colony management,… This practical course will be co-sponsored by Hamilton-Thorne, ParaTechs, Sutter/Primetech. Registration for this 3-days practical workshop must be done through tt2013@transtechsociety.org and the TT2013 meeting registration web page. Workshop cost: 400$ (USD).

Registration fee to attend the TT2013 meeting begins at 400$(USD) and extends up to 630$(USD). ISTT members and technician/student participants are entitled to reduced registration fees.

Accommodation is NOT included in the registration fee and lodging must be reserved independently at the Guangzhou Baiyun International Convention Center, where a limited number of rooms have been prebooked for participants attending the TT2013 meeting at convenient reduced prices (610 RMB-single/680 RMB-double room). Note: RMB are China Yuan Renminbi, 100 RMB ~ 16 USD/12 Euros. Please check for most updated currency exchange.

The International Society for Transgenic Technologies (ISTT) will be sponsoring several Registration Awards for ISTT members willing to attend the TT2013 meeting. Applications for ISTT Registration Awards to attend the TT2013 meeting will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Registration Awards.

The International Society for Transgenic Technologies (ISTT), in collaboration with inGenious Targeting Laboratory (iTL), has establised the ISTT YOUNG INVESTIGATOR AWARDthat will be given at the Transgenic Technology (TT) Meetings. Nominations for the 2nd ISTT Young Investigator Award, for the TT2013 meeting, will be accepted up to October 15, 2012. Please refer to instructions provided at the corresponding ISTT web page for ISTT Young Investigator Awards.

Sponsoring companies willing to support the TT2013 meeting and to exhibit at the TT2013 meeting venue should contact TT2013 organizers through the TT2013 meeting email address (tt2013@transtechsociety.org). TT2013 meeting sponsorship opportunities are available at the meeting web site.

TT2013 meeting deadlines

Abstract submission deadline:  October 15, 2012
Application for ISTT registration awards:  October 15, 2012
Awards to be communicated by October 30, 2012
Early bird registration deadline: October 15, 2012
Standard fee registration deadline: January 31, 2013
Late registration fees & on-site registration: February 01, 2013

See you all in Guangzhou, at the TT2013 meeting!

Lluis Montoliu, PhD; President of the ISTT

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Posted in animal biotechnology, animal experimentation, co-sponsorhip, course, embryos, ES cells, general, information, iPS cells, ISTT, ISTT booth, ISTT Members, ISTT Prize, knock-outs, knockout, meetings, practical course, registration awards, rodent genetics, sperm, transgenic research, travel awards, TT meeting, web site, workshop, Young Investigator Award | No Comments »

EMMA Cryopreservation Workshop in Madrid: a meeting report

Friday, May 11th, 2012
EMMA Cryopreservation Workshop: a meeting report

EMMA Cryopreservation Workshop: a meeting report

The EMMA (European Mouse Mutant Archive) Cryopreservation Workshop took place earlier this week in Madrid, May 7-8, at the main campus of CSIC, with an excellent success, organized by EMMA, supported by the EC-7th Framework Programme and co-sponsored by the International Society for Transgenic Technologies (ISTT). Sixty participants from many countries around the world gathered to present and discuss, in depth, the latest approaches, methodologies and techniques available for the efficient cryopreservation of mouse strains, through embryo, sperm and ovary cryopreservation. In addition to invited speakers and invited participants, the workshop was attended by delegates from EMMA nodes and 12 ISTT members. As many as 21 talks were delivered, by selected invited speakers, representing the different major archiving iniatives currently existing (EMMA, MMRRC, The Jackson Laboratory, RIKEN, APN, CMMR, AMMRA) and the research and development initiatives, as well as state-of-art protocols in the field. Presentations, abstracts and pictures from this EMMA Cryopreservation Workshop are freely available to anyone interested, from the meeting web site, and can be also accessed from ISTT and EMMA web sites. The remaining presentations will be progressively added upon receiving the approval from the corresponding guest speakers.

At EMMA, we envisaged this cryopreservation workshop as a forum to brainstorm and discuss in depth the latest technological advances in cryopreservation, including sperm and embryo cryopreservation, updated IVF methods and related techniques as ovary cryopreservation, laser-assisted and piezo-driven ICSI, transportation of frozen material and other technical and logistic challenges relevant to the operation of current mouse embryo/sperm archives. We believed we entirely fulfilled the expectations and all participants went back home, to their research institutions, loaded with new ideas, updated solutions and suggested improvements that can be explored and applied for a most efficient management of a mouse embryo/sperm cryopreservation bank. All participants agreed to continue organizing this type of focused workshops in the near future. The ISTT will be always there, ready to support these very interesting initiatives.

Lluis Montoliu, EMMA Spanish node

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Posted in co-sponsorhip, cryopreservation, database, embryos, ES cells, facility, ISTT, knock-outs, knockout, Meeting report, rodent genetics, service, sperm, transgenic research, web site, workshop | No Comments »

Genetic differences among C57BL/6 mouse strains available from the Mouse Phenome Database

Monday, April 9th, 2012
Genetic differences among C57BL/6 mouse strains
Genetic differences among C57BL/6 mouse strains

The Mouse Phenome Database (MPD), at the Jackson laboratory web site, has recently updated their list of SNP polymorphisms for closely related strains across the entire mouse genome. The premade lists, genome-wide, ready-to-use, now include three NEW comparisons containing all currently known genetic differences (SNPs) among C57BL/6 strains, particularly between C57BL/6J and C57BL/6N mouse strains, and their related substrains (entire genome occurrences).

  1. C57BL/6J versus C57BL/6NJ

    2. (coding and UTR regions only, 4341 SNPs)

  2. C57BL/6J versus C57BL/6NJ (all available SNPs that are polymorphic, 152001 SNPs)
  3. 13 C57BL/6 mouse related strains, J and N, including: C57BL/6J, C57BL/6ByJ, C57BL/6JArc, C57BL/6JBomTac, C57BL/6JCrl, C57BL/6JEiJ, C57BL/6JOlaHsd, C57BL/6JRccHsd, C57BL/6NCrl, C57BL/6NHsd, C57BL/6NJ, C57BL/6NNIH, C57BL/6NTac

The entire community of researchers in biomedicine using mouse genetics greatly appreciates the most useful service and helpful web resources provided by the MPD team. Thanks a lot colleagues!

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World Map of Transgenic Core Facilities

Saturday, April 7th, 2012
World Map of Transgenic Core Facilities

World Map of Transgenic Core Facilities

At the International Society for Transgenic Technologies (ISTT) web site, according to the aims of our Society. we care to provide the entire scientific community with as much information as possible regarding how and where to generate genetically modified animals, particularly transgenic and knockout mice, as useful animal models for research projects in biology, biotechnology and biomedicine. One of these resources of information is the World Map of Transgenic Core Facilities, currently holding more than 125 links to web sites of transgenic core facilities located in 27 countries, world wide. The transgenic core facilities can be easily found in a list, arranged per country, or using a useful Google Maps built-in feature depicting the geographic location of each transgenic facility.

Is your transgenic core facility not yet listed in the World Map of Transgenic Core Facilities offered from the ISTT web site? No problem. Whether public or private, whether based on an academic environment or associated with a company, all transgenic core facilities, all initiatives meant to produce transgenic animals (mice, rats, other mammals, other vertebrates,…) on demand, for research purposes, are welcome and we, at the ISTT, will be pleased to include all these links in our web site. Please contact us at istt@transtechsociety.org and send us your web link and contact details of your transgenic core facility and we will be more than happy to add your transgenic core facility to the list of World Map of Transgenic Core Facilities.

Thanks for submitting the web site of your Transgenic Core Facility to the ISTT.

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Posted in database, embryos, ES cells, facility, general, genes, information, knock-outs, knockout, list, rodent genetics, transgenic, transgenic research, web site | No Comments »

EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

Thursday, February 16th, 2012
EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

EMMA Cryopreservation Workshop, Madrid, Spain, 7-8 May 2012

The International Society for Transgenic Technologies (ISTT) co-sponsors the EMMA Cryopreservation Workshop, organized by EMMA (the European Mouse Mutant Archive) and CSIC (the Spanish Research Council) and to be held at the CSIC Main Campus, in Madrid (Spain), on 7-8 May 2012. The Organizers of this workshop are Martin Fray (Mammalian Genetics Unit, MRC, Harwell, UK; Biological Resources Manager at the EMMA node in MRC-MGU-Harwell and ISTT Member), Michael Hagn (Institute of Experimental Genetics, HMGU, Munich, Germany; EMMA Project Manager) and Lluis Montoliu (National Center of Biotechnology, CNB-CSIC, Madrid, Spain; Spanish EMMA node co-ordinator and ISTT Member).  The Organizers have selected a number of speakers in the field and hot topics in cryopreservation with a main focus on technology. The main aim of this workshop is to discuss openly all topics at length and in depth, from past and present initiatives, undertaken by the various archiving initiatives world-wide, invited to this workshop, to the current and future challenges all bio-repositories have to face and address adequately. Topics that will be discussed include: embryo and organ cryopreservation, sperm cryopreservation and IVF, ICSI, mouse production, morula/ES aggregation method, transportation issues with frozen and unfrozen biological material and various continental efforts towards cryopreservation. Additional information can be obtained from workshop web site.

Confirmed invited participants include:

  • Sue Bath (Melbourne, Australia)
  • Martina Crispo (Institut Pasteur, Montevideo, Uruguay)
  • Xiang Gao (Model Animal Research Center, Nanjing University, Nanjing, P.R. China)
  • Marina Gertsenstein (Toronto Centre for Phenogenomics, Toronto, ON, Canada)
  • Alan Hart (MRC-Human Genetics Unit at MRC/IGMM, University of Edinburgh, Edinburgh, UK)
  • Jean Jaubert (Institut Pasteur, Paris, France)
  • Carlisle Landel (Thomas Jefferson University, Kimmel Cancer Center, Philadelphia, PA, USA)
  • Kent Lloyd (Mouse Biology Program, University of California, Davis, CA, USA)
  • Peter Mazur (The University of Tennessee, Knoxville, TN, USA)
  • Keiji Mochida (RIKEN Bioresource Center, Tsukuba-shi, Ibaraki, Japan)
  • Pedro Moreira (European Molecular Biology Laboratory, Monterotondo/Rome, Italy)
  • Naomi Nakagata (Center for Animal Resources & Development-CARD, Kumamoto University, Kumamoto, Japan)
  • Lauryl Nutter (Toronto Centre for Phenogenomics, Toronto, ON, Canada)
  • Atsuo Ogura (RIKEN Bioresource Center, Tsukuba-shi, Ibaraki, Japan)
  • Sagrario Ortega (National Cancer Research Center, CNIO-ISCIII, Madrid, Spain)
  • Belén Pintado (National Center of Biotechnology, CNB-CSIC, Madrid, Spain)
  • Marcello Raspa (EMMA-CNR, Monterotondo/Rome, Italy)
  • Stuart Read (The Australian National University, The Australian Phenomics Facility, Canberra, Australia)
  • Jorge Sztein (CMB Cryopreservation and Assisted Reproduction, NIAID-NIH, Rockville, MD, USA)
  • Rob Taft (The Jackson Laboratory, Bar Arbor, ME, USA)
  • Toru Takeo (Center for Animal Resources & Development-CARD, Kumamoto University, Kumamoto, Japan)
  • Xavier Warot (Center of PhenoGenomics, School of Life Sciences, EPFL, Lausanne, Switzerland)
  • Michael Wiles (The Jackson Laboratory, Bar Arbor, ME, USA)

In addition, the EMMA Cryopreservation workshop will be attended by delegates from EMMA nodes and up to ten (10) current* ISTT members selected among applicants with expertise in cryopreservation, on behalf of the EMMA-ISTT cooperation agreement, for mutual promotion and collaboration, currently in place, and thanks to the specific co-sponsorship of the ISTT agreed by the ISTT council for this EMMA workshop. This is a highly specialized workshop with about 60 participants, all attending by invitation. However, up to ten ISTT members with expertise in cryopreservation will be able to benefit and attend. ISTT members interested and willing to attend this meeting can submit their applications to Lluis Montoliu (montoliu@cnb.csic.es), including a CV and a letter describing how the applicant will benefit from attending the workshop, but also how the applicant will be able to contribute to discussions. Applications should be sent by email to Lluis Montoliu by February 29th, 2012. Thereafter, Organizers will review all received applications and select up to ten ISTT Members with expertise in cryopreservation. All invited participants will be encouraged to actively take part in all discussions throughout the meeting. There is no registration fee for this workshop. Selected ISTT members will be entitled to one night accommodation (7 to 8 May 2012) on behalf of the Organization of this workshop. All participants will be invited to the official Workshop dinner on May 7.

Also, in an effort to disseminate the outcome of this workshop among interested colleagues, the Organizers will make the workshop abstracts, submitted by invited speakers, and some/all of speakers’ slides available on the public EMMA and ISTT web sites, after obtaining the specific permission to share material from each invited speaker.

(*) current ISTT members are those registered/renewed in 2012.

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