Archive for the ‘workshop’ Category

Requesting proposals to host the 13th Transgenic Technology (TT2016) meeting

Monday, January 27th, 2014
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Requesting proposals to host the 13th Transgenic Technology (TT2016) meeting

Requesting proposals to host the 13th Transgenic Technology (TT2016) meeting

Dear ISTT members,

We are pleased to invite your proposals for hosting the 13th Transgenic Technology Meeting (TT2016) in February-March 2016. The updated ISTT bylaws, which were approved at the TT2013 meeting in China, now allow proposals to be received from anywhere in the world, without the requirement for a rotation between regions. Therefore, ISTT members from all continents are encouraged to consider hosting the TT2016 meeting in their city, with the support of their institution(s).

Please note that only ISTT members are entitled to submit proposals to host a TT meeting.

Important points to be addressed in any submitted proposal:

  1. The proposal must have the support of the hosting institution(s). Letters of support from the corresponding director(s) of organizing institution(s) must be provided.
  2. The hosting institution(s) are fully liable for the organization of the meeting, including all economic aspects.
  3. A proposed preliminary budget should be included in the proposal, along with suggested registration fees.
  4. A preliminary program for the TT2016 meeting, including topics (not necessarily speakers) and proposed workshops should be provided. This program should take into account the topics and speakers invited at previous TT meetings, avoiding unnecessary repetition. Full information regarding previous TT meetings organized is available at the members-only area, within the “meetings” tab.
  5. Proposed venue and dates for the TT2016 should be indicated. Exact sites and dates might be subjected to change later, if required, after obtaining the approval from the ISTT council. Information regarding the venue and/or the city where the TT2016 meeting would take place is always desirable as is proof that the venue will be available at the scheduled dates.
  6. Proposed committees should be presented, which should include at a minimum: Organizing Committee, Scientific Advisory Committee.
  7. A meeting Chair (who must be ISTT member) should be clearly identified.
  8. Since TT meetings are the most important activity of the ISTT, the President and other members of the ISTT Council have to be involved in committees and collaborate with local organizers in defining the final program.
  9. Upon selection, a contract will have to be signed between the hosting institution(s) and the ISTT
  10. Information regarding suggested accommodation facilities (and prices) for participants should be provided.
  11. The expected organization of a hands-on workshop on a selected topic, to take place immediately before or after the meeting, is always desirable.
  12. The involvement of a professional meeting organizer is desirable but not essential if there are viable alternatives, such as institutional meeting support staff.
  13. Information on accessibility of the city from international airports as well as between the conference venue and accommodation should be provided. A list of hotels close to the conference including price range is desirable.
  14. Finally, an outline of the proposed social activities should be included.

We look forward to receiving interesting proposals from all over the world. Please submit your proposal (ideally all information organized into a single PDF document) to istt@transtechsociety.org by Friday 27 June 2014. The evaluation committee might contact any proponent in order to request any additional/missing information that would be required to better assess the proposal. The selected venue will be announced by September 30, 2014, and the Chair of the selected proposal will be kindly invited to introduce the highlights of the TT2016 meeting at the closing ceremony of the TT2014 meeting in Edinburgh.

Thanks for your due consideration of this message,

Boris Jerchow
Jorge Sztein
Karen Brennan

ISTT Council sub-committee
in charge of evaluating TT2016 proposals

Updated scientific and workshop programmes for the TT2014 meeting in Edinburgh

Friday, January 10th, 2014
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Upades scientific and workshop programmes for TT2014 meeting in Edinburgh: Please, register today!.

Upades scientific and workshop programmes for TT2014 meeting in Edinburgh: Please, register today!.

The scientific and zebrafish transgenesis hands-on workshop programmes prepared for the 12th Transgenic Technology (TT2014) meeting, to be held in Edinburgh (Scotland, UK), on October 6-8 (workshop on October 8-10) 2014,  have been recently updated by the Organizers, chaired by Douglas Strathdee (Glasgow, UK). These rewarding updates further increased the already high quality and interest for this popular conference series, promoted from the International Society for Transgenic Technologies (ISTT), the most important forum where to discuss the state-of-art of animal transgenic technology, to share new developments, to review the deployment of the new methods that have recently being devised and, in summary, an excellent arena where to easily meet, face-to-face, the most relevant key-players in the field while providing a wonderful excuse to gather and ex-change experiences with the entire ISTT family of members.

The updated list of confirmed invited speakers attending the TT2014 meeting (6-8 October 2014) includes:

  • David Adams, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
  • Ignacio Anegon, Center for Research in Transplantation and Immunology, Nantes, France
  • James Bussell, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
  • Ian Chalmers, MRC Centre for Regenerative Medicine, The University of Edinburgh, UK
  • Stephen Ekker, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
  • Anna-Katerina Hadjatonakis, Developmental Biology Program, Sloan-Kettering Institute, New York, USA
  • Peter Hohenstein, The Roslin Institute and Royal Dick School of Vetinary Studies & MRC IGMM, University of Edinburgh, UK
  • Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Nine Cambridge Center Cambridge, USA
  • Jos Jonkers, Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
  • Keith Joung, Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA
  • Alexandra Joyner, Developmental Biology Program, Sloan-Kettering Institute, New York, USA
  • Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics, Shizuoka, Japan
  • Michael McGrew, Division of Developmental Biology, The Roslin Institute and Royal Dick School of Veterinary Studies, University of Edinburgh, UK
  • Daniel J Murphy, Beatson Institute for Cancer Research, University of Glasgow, UK
  • James Murray, Department of Animal Science and Department of Population Health and Reproduction, University of California, Davis, California, USA
  • Stephen Murray, The Jackson Laboratory, Bar Harbor, Maine, USA
  • Lluis Montoliu, ISTT President, Organising Committee, National Center of Biotechnology (CNB), CSIC, Madrid, Spain
  • Vasilis Ntziachristos, Technische Universität Mu?nchen, Munich, Germany
  • Elizabeth Patton, MRC Human Genetics Unit & MRC IGMM, University of Edinburgh, UK
  • Pawel Pelczar, Institute of Laboratory Animal Science, Zürich, Switzerland
  • Jan-Bas Prins, Leiden University Medical Centre, The Netherlands
  • Janet Rossant, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada (ISTT Prize)
  • Angelika Schnieke, Livestock Biotechnology, WZW Center of Life Science, Freising-Weihenstephan, Germany
  • Kai Schönig, Central Institute of Mental Health, Heidelberg University, Mannheim, Germany
  • William Skarnes, Wellcome Trust Sanger Institute, Hinxton, Cambridge, UK
  • Austin Smith, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK
  • Francis Stewart, Biotechnology Center of the TU Dresden, Germany
  • Sara Wells, MRC Harwell, Oxfordshire, UK
  • Jacqueline White, Wellcome Trust Sanger Institute, Hinxton, Cambridge UK

The updated list of confirmed invited speakers & instructors attending the hands-on zebrafish transgenesis workshop taking place immediately after the TT2014 meeting (8-10 October 2014) includes:

  • Liz Patton, MRC Institute of Genetics and Molecular Medicine, The University of Edinburgh
  • Carl Tucker, Biomedical Research Resources, The University of Edinburgh
  • Tim Czopka, Centre for Neuroregeneration, The University of Edinburgh
  • Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics, Shizuoka, Japan
  • Stephen Ekker, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
  • Keith Joung, Department of Pathology, Massachusetts General Hospital and Harvard Medical School
  • Henry Roehl, Department of Biomedical Science, The University of Sheffield
  • Robert Kelsh, Centre for Regenerative Medicine and Department of Biology and Biochemistry, The University of Bath
  • Martin Denvir, The University of Edinburgh/British Heart Foundation Centre for Cardiovascular Science, The University of Edinburgh
  • David Lyons, Centre for Neuroregeneration, The University of Edinburgh
  • Dirk Seiger, Centre for Neuroregeneration, The University of Edinburgh
  • Karthikeyani Paranthaman, MRC Institute of Genetics and Molecular Medicine, The University of Edinburgh

Abstracts: All TT2014 participants are encouraged to submit their work as an abstract for poster presentation at the TT2014 meeting. Abstracts should be submitted no later than June 30, 2014. Accepted abstracts will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated. A limited number of abstract submissions will be selected and invited to present their findings in the form of a short oral presentation within the main meeting program. Abstracts are invited on all aspects of Transgenic Technologies, including the conference themes as listed below:

  • New technologies in animal transgenesis
  • Embryo stem cells
  • Target nucleases or Editing nucleases (ZFNs, TALENs, CRISPRs)
  • Large-scale phenotyping
  • Animal Biotechnology
  • Imaging with transgenic animals
  • Mouse models of human disease
  • Zebrafish models of human disease and transgenesis
  • Animal ethics and welfare

Registration for both the TT2014 meeting and the zebrafish transgenesis workshop are OPEN. Registration for the TT2014 meetings starts at 265 UK Pounds for technician/student ISTT members and progressively increases for the rest of categories of delegates. ISTT members are always entitled to reduced registration fees. Registration for the zebrafish transgenesis workshop is independent, with an extra cost of 275 UK Pounds , and only open to delegates that have also registered to attend the TT2014 meeting. The early bird reduced registration fees are operative until July 31, 2014. Thereafter, registration will be progressively become more expensive. Hence,  please register by July 31, 2014 to benefit from reduced registration fees.

ISTT Registration Awards: Application to ISTT registration awards for the TT2014 meeting is OPEN. A minimum of six registration awards for ISTT members will be sponsored by the International Society for Transgenic Technologies (ISTT). Applications should be sent, along with the registration confirmation and the requested additional documents to istt@transtechsociety.org by June 30, 2014. The ISTT will pay the Registration Fee of all applicants selected for an award. Please note that applicants not selected for an award are required to pay the coresponding registration fee. Please note the Award covers registration fees and attendance at all social events, however, does not cover travel, accommodation expenses or attendance at pre meeting events. Award decisions will be communicated by July 15, 2014 and awardees will receive a diploma at the TT2014 Meeting. Deadline for submitting application for ISTT Registration Awards for TT2014: 30 June 2014. Registration Award decisions will be communicated by 15 July 2014.

Looking forward to meeting you all in Edinburgh!

 

The ISTT attended the 50th Anniversary LASA Meeting

Thursday, November 28th, 2013
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The ISTT attended the 50th Anniversary LASA meeting

The ISTT attended the 50th Anniversary LASA meeting

The President of the International Society for Transgenic Technologies (ISTT), Lluís Montoliu, was kindly invited, to attend the 50th Anniversary (1963-2013) meeting of the Laboratory Animal Science Association (LASA), held  in London (UK), this week. A workshop on “Transgenic Science and Technology” was organized, where various ISTT members participated as Organizers, invited speakers or Chairs of the sessions. This workshop included a presentation on the “Evolution of the ISTT“, delivered by Lluis Montoliu and describing the short (2006-2013), but intense and most successful, history of the ISTT, since its inception in Spain to date, the previous history of the TT meetings and all the activities, projects and events undertaken, organized, promoted or sponsored by the ISTT over these past few years.  Numerous ISTT, and LASA, members were present among the audience.

Transgenic Technologists form a network across Oceania

Friday, November 22nd, 2013
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Transgenic Technologists form a network across Oceania

Transgenic Technologists form a network across Oceania

The 1st Oceania Transgenic/Cryopreservation Symposium took place at the University of Queensland, Brisbane Australia on the 31st October -1st November 2013. This meeting was initiated by Elizabeth Williams and has grown out of workshops on cryopreservation of reproductive materials and in-vitro fertilization (IVF) that were run in conjunction with ANZLAA annual conferences (Hobart 2011 & Brisbane 2012). Through these activities it was recognized that there was a need for more local networking, collaboration and communication amongst transgenic technologists across Australia and New Zealand.

The meeting drew 53 participants representing twenty-six different organisations. 14 of the participants were locals from the Brisbane area, but there were also 33 interstate and 6 international delegates; mostly from New Zealand, but with one colleague visiting from Japan. The Australian Institute for Biotechnology and Nanotechnology generously provided the meeting venue and facilities.

The organizers Elizabeth Williams (University of Queensland Biological Resources, Brisbane), Karen Brennan (Victor Chang Cardiac Research Institute, Sydney), Andrew Brown (University of Auckland, Auckland), Paul Scowen (University of Tasmania, Hobart) and Kevin Taylor (Australian Bioresources, Moss Vale), all ISTT members, had canvassed topics from potential participants ahead of the meeting and ran informal discussions under the session headings Cryopreservation, Transgenic and Knock-Out Production, Administrative Issues, Husbandry, New Technologies/Techniques, and Rederivation.

Read the full meeting report prepared by ISTT council member Karen Brennan.

Transgenic Technologists form a network across Oceania

Transgenic Technologists form a network across Oceania

The TT2014 meeting web page has been launched: REGISTRATION IS OPEN!

Thursday, October 31st, 2013
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The TT2014 meeting web site has been launched. REGISTRATION IS OPEN!

The TT2014 meeting web site has been launched. REGISTRATION IS OPEN!

Today, the 12th Transgenic Technology (TT2014) meeting web site has been launched. And meeting registration is already open!. The TT2014 meeting is organized by ISTT members Douglas Strathdee-chair, Peter Hohenstein and Bruce Whitelaw and will be held at The Assembly Rooms, in Edinburgh, Scotland, UK, on 6-8 October 2014. Immediately following the TT2014 meeting, on October 9-10, 2014, there will be a hands-on practical workshop called ‘An Introduction to Zebrafish Transgenesis‘ which will focus on Zebrafish.  Further details about this practical workshop will be announced at the TT2014 meeting web site.

The meeting is hosted by three world-class Scottish research institutes and the University of Edinburgh: the Roslin Institute; the Institute of Genetics and Molecular Medicine and the Beatson Institute for Cancer Research. All three Institutes are world-renowned for producing top quality science at the forefront of biomedical research. The TT meeting visits the UK for the first time following the previous TT meetings in Guangzhou, China (TT2013); Florida, USA (TT2011); Berlin, Germany (TT2010); Toronto, Canada (TT2008); Brisbane, Australia (TT2007) and Barcelona, Spain (TT2005). This will be the 12th meeting in the series, originally pioneered by Johannes Wilbertz (Karolinska Institute, Stockholm, Sweden) in 1999. Since the foundation of the ISTT in 2006, the TT meetings have been the main event sponsored by the Society.

The following speakers have confirmed their participation at the TT2014 meeting:

  • David Adams, Wellcome Trust Sanger Institute, Hinxton, Cambridge UK
  • Ignacio Anegon, Center for Research in Transplantation and Immunology, Nantes, France
  • Stephen Ekker, Department of Biochemistry and Molecular Biology, Mayo Clinic, Rochester, Minnesota, USA
  • Kat Hadjatonakis, Developmental Biology Program, Sloan-Kettering Institute, New York, USA
  • Coenraad Hendriksen, Institute for Translational Vaccinology, Bilthoven, The Netherlands
  • Rudolf Jaenisch, Whitehead Institute for Biomedical Research, Nine Cambridge Center Cambridge, USA
  • Jos Jonkers, Division of Molecular Pathology, The Netherlands Cancer Institute, Amsterdam, The Netherlands
  • Keith Joung, Molecular Pathology Unit, Massachusetts General Hospital, Charlestown, MA, USA
  • Alex Joyner, Memorial Sloan-Kettering Cancer Center, New York, NY, USA
  • Koichi Kawakami, Division of Molecular and Developmental Biology, National Institute of Genetics, Shizuoka, Japan
  • Jim Murray, Department of Animal Science and Department of Population Health and Reproduction, University of California, Davis, California, USA
  • Stephen Murray, The Jackson Laboratory, Bar Harbor, Maine, USA
  • Lluis Montoliu, ISTT President, Organising Committee, National Center of Biotechnology (CNB), CSIC, Madrid, Spain
  • Vasilis Ntziachristos, Technische Universität Mu?nchen, Munich, Germany
  • Pawel Pelczar, Institute of Laboratory Animal Science, Zürich, Switzerland
  • Janet Rossant, The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada
  • Angelika Schnieke, Livestock Biotechnology, WZW Center of Life Science, Freising-Weihenstephan, Germany
  • Kai Schönig, Central Institute of Mental Health, Heidelberg University, Mannheim, Germany
  • Austin Smith, Wellcome Trust-Medical Research Council Stem Cell Institute, University of Cambridge, Cambridge, UK
  • Sara Wells, MRC Harwell, Oxfordshire, UK
  • Jacqui White, Wellcome Trust Sanger Institute, Hinxton, Cambridge UK

At the TT2014 meeting, the ISTT will be awarding the 10th ISTT Prize for outstanding contributions to the field of transgenic technologies to Prof. Janet Rossant (The Hospital for Sick Children, University of Toronto, Toronto, Ontario, Canada). The ISTT Prize is generously sponsored by genOway.

At the TT2014 meeting, the ISTT will be also awarding the 3rd ISTT Young Investigator Award, generously sponsored by inGenious Targeting Laboratory. The ISTT Young Investigator Award recognizes outstanding achievements by a young scientist who will keep the field of transgenic technologies vibrant with new ideas and who has recently received his or her advanced professional degree.

At the TT2014 meeting, and for the first time, the ISTT Best Poster Awards, traditionally awarded to the best posters presented at the corresponding TT meeting, will be generously sponsored by Charles River.

Accepted abstracts submitted for the TT2014 meeting, will be published in the scientific journal Transgenic Research (Springer), to which the ISTT is associated.

A minimum of six registration awards for ISTT members will be sponsored by the International Society for Transgenic Technologies. Applications should be sent, along with the registration document to istt@transtechsociety.org by June 30, 2014. Award decisions will be communicated by July 15, 2014 and awardees will receive a diploma at the TT2014 Meeting.

Important deadlines:

  • Abstract submission deadline June 30, 2014
  • Application for ISTT registration awards deadline June 30, 2014
  • Awards to be communicated by July 15, 2014
  • Early Bird registration fee deadline July 31, 2014
  • Standard Rate registration fee from August 1, 2014
  • Late & On-Site Rate registration fee from September 22, 2014

As it is stated in the TT2014 meeting home page: “Scotland prides itself on both its life science research and the warm welcome given to visitors and looks forward to hosting TT2014“. Therefore, on behalf of the ISTT and of the TT2014 Organising Committee we invite you all to attend to the TT2014 meeting.

See you all in Edinburgh!

Workshop report: animals bred, but not used in experiments

Wednesday, October 23rd, 2013
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Workshop:  “Animals bred, but not used in experiments”, October 18-20, 2013, Hotel Duin & Kruidberg, Santpoort, the Netherlands (Picture kindly provided by Fernando Benavides)

Workshop: “Animals bred, but not used in experiments”, October 18-20, 2013, Hotel Duin & Kruidberg, Santpoort, the Netherlands (Picture kindly provided by Fernando Benavides)

Workshop: “Animals bred, but not used in experiments”, October 18-20, 2013, Hotel Duin & Kruidberg, Santpoort, the Netherlands.

Experiments in biomedical science use large numbers of laboratory animals. It is a fact that to provide these animals, regularly more animals are bred than are finally used in the experiments planned. The Ministry of Economic Affairs as the competent body of the Netherlands had asked Prof. Coenraad Hendriksen and Dr. Jan-Bas Prins to organize a workshop to identify the reasons for the breeding of surplus animals and to devise recommendations as to how the number of animals that are bred but not used can be reduced to a minimum.

A number of experts from different fields of laboratory animal science were invited for a two day workshop to the Hotel Duin & Kruidberg in Santpoort, a town close to Amsterdam, to discuss these issues and to develop a paper for the Dutch authorities. Obviously, many of the laboratory animals bred are genetically altered (GA) animals. Moreover, techniques to cryopreserve GA animal lines could be a means to reduce the number of animals that are bred. The invitation was therefore extended to the ISTT to send a representative to take part in this workshop.

Here, I will give a short summary of the topics that have been discussed and of the outcomes. However, I refer you to the final report of the workshop, parts of which have been developed within individual small workgroups and will be put together into a final document by the kind efforts of Coenraad and Jan-Bas. I will inform you immediately upon the publication of this report.

A topic central to the discussion was the identification of reasons for the production of animals that are then not used in experiments. A major reason for this is the production of unwanted sexes and unwanted genotypes. The participants agreed that good planning can considerably reduce the number of surplus animals. At the same time, resources can be saved and either used for additional experiments or for cost reduction. However, breeding schemes with multiple alleles, as well as the organization of a facility, can be complex. A strong need for counseling as well as education of users of laboratory animals was identified, to make them competent to plan accordingly. The centralization of the breeding colonies under the responsibility of the facility management was discussed as a possibility to streamline breeding strategies. On the other hand, for the time being, this does not seem to be feasible for very many facilities. Local Animal Welfare Committees should evaluate local SOPs and develop a catalogue of best practices to help keep surplus animals to a minimum. GA animal lines should be cryopreserved immediately after their creation when there is no need to breed extra animals for this purpose and when animals from test rederivations can be used for experiments or for the breeding colony. Thereby, the lines are protected from disaster and from genetic drift at the same time, live mice can be terminated at any time, and the lines can be easily shipped to collaborators. Lines should be made available to collaborators as early as possibly to avoid generating the same line at different places. In case expertise for cryopreservation is lacking, lines can be donated to repositories like EMMA where they are cryopreserved free of charge. Investigators should always consider sharing lines with the scientific community through such repositories.

A second important topic discussed during the workshop was the use of new technologies for the generation of GA animals as well as for their experimental analysis. New lines should be directly generated on the desired background. In case backcrossing is needed, speed congenic strategies should be used to reduce the number of animals needed during that process. Technologies utilizing the targeting of nucleases to the locus of interest (ZFNs, TALENs, CRISPER/Cas9) promise to eventually allow the generation of GA lines with reduced numbers of animals directly on the desired background. Complex strategies for the generation of customized animals for specific experiments were presented. It was agreed that these should be freely available. However, individual scientists and institutes should evaluate whether it is worth adopting a new and complicated technique. Since the process of setting up complex protocols may well lead to the use of high numbers of animals, investigators should consider collaborating with colleagues who perform similar experiments at large scales.

Ethical considerations let us come to the understanding that there is an intrinsic value of life. We found that it is for this reason that it is morally wrong to kill more animals than absolutely necessary. Biomedical science is tasked with producing answers to pressing questions on the molecular functions of life and disease and finding new cures. It was pointed out that the principles of the 3R’s have to be respected at all times, but a number of animal experiments are indispensable. In this context, it is unavoidable to breed animals that are not used for these experiments, but it is important to ensure that their numbers are kept to a minimum.

Boris Jerchow
Member of ISTT’s Executive Council
October 23, 2013

List of participants and affiliations, excluding those who were unable to send permission for disclosure:

van der Broek, Frank, NVWA, The Netherlands; Aleström, Peter, The Norwegian Zebrafish Platform, Norway; Benavides, Fernando, University of Texas, USA*; Bussell, James, Wellcom Trust Sanger Institute, UK*; Chrobot, Nichola, MRC Harwell, UK; van Es, Johan, Hubrecht University, The Netherlands; Fentener van Vlissingen, Martje, Erasmus MC, The Netherlands; Hendriksen, Coenraad, InTraVacc, The Netherlands; Hohenstein, Peter, Roslin Intitute, UK*; Krimpenfort, Paul, NKI, The Netherlands; Morton, David, UK; Prins, Jan-Bas, LUMC, The Netherlands; Raspa, Marcello, EMMA, Italy*; Tramper, Ronno, Consultant, The Netherlands; van der Valk, Jan, NKCA; Wilbertz, Johannes, Karolinska Institutet, Sweden*; Ohl, Frauke, Utrecht University, The Netherlands; Pool, Chris, KNAW, The Netherlands; Witler, Lars, Max-Planck Institute Mol. Gen., Berlin, Germany*.

* ISTT members

Workshop: “Animals bred, but not used in experiments”, October 18-20, 2013, Hotel Duin & Kruidberg, Santpoort, the Netherlands (Picture kindly provided by Fernando Benavides)

Workshop: “Animals bred, but not used in experiments”, October 18-20, 2013, Hotel Duin & Kruidberg, Santpoort, the Netherlands (Picture kindly provided by Fernando Benavides)

CARD-CNB Cryopreservation Course Report

Monday, October 14th, 2013
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Organizers, instructors, lecturers and participants at the CARD-CNB Cryopreservation Course, held at CNB-CSIC in Madrid, Spain, on 7-11 October 2013 and organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain)

Organizers, instructors, lecturers and participants at the CARD-CNB Cryopreservation Course, held at CNB-CSIC in Madrid, Spain, on 7-11 October 2013 and organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain)

This past week, 7-11 October 2013, the CARD-CNB Mouse Sperm and Embryo Cryopreservation Course was held at CNB-CSIC, in Madrid, Spain, with great success and accompanied with also great sunny weather. This was the first cryopreservation course of this kind, co-organized by Naomi Nakagata (CARD-University of Kumamoto, Japan) and Lluis Montoliu (CNB-CSIC, Madrid, Spain), were the newest methods developed by CARD, at the Nakagata lab, were demonstrated in Europe, directly by the CARD team. The instructors at this CARD-CNB course were commanded by the CARD-University of Kumamoto team, from Japan (Toru Takeo, Kiyoko Fukumoto, Tomoko Kondo, Yukie Haruguchi, Yumi Takeshita, Yuko Nakamuta and Shuuji Tsuchiyama), and additional help and collaboration was provided from the Mouse Biology Program at UC-Davis, CA, USA (Kristy Kinchen), from INIA, Madrid, Spain (Raúl Fernández), from CIEMAT, Madrid, Spain (Jesús Martínez), from USA (Jorge Sztein), from Paratechs, Lexington, KY, USA (Barbara Stone) and from CNB-CSIC (Julia Fernández, María Jesús del Hierro, Marta Castrillo and Isabel Martín-Dorado), for several of the methods demonstrated. All instructors must to be praised for their deep knowledge, patience and extraordinary dedication and commitment towards the success of this course. Complementary and most interesting lectures were provided on a wide variety of topics related to the course main focus, including: animal welfare and regulations by Belén Pintado and Jorge Guillén; the history, fundaments and comparison of methods by Jorge Sztein; the effects of the in vitro culture of mouse embryos by Alfonso Gutiérrez-Adán; safety and handling issues of liquid nitrogen by Jesús Martínez; shipping frozen and refrigerated materials by Toru Takeo, databases in a cryopreservation lab, by Shuuji Tsuchiyama, about EMMA by Lluis Montoliu and CARD by Naomi Nakagata, as examples of mouse embryo and sperm archives, and, also, on the new editing nucleases for genome modification, by Kai Schönig (Mannheim, Germany), a talk sponsored by Sigma.

As many as 24 participants, coming from research institutions or companies located in 16 countries around the world (UK, Spain, Australia, USA, Canada, Czech Republic, Brazil, Finland, France, Denmark, Israel, Netherlands, Portugal, Sweden, Italy and Taiwan) were presented with the latest advances in mouse sperm and embryo cryopreservation and all associated mouse reproductive biology ancillary techniques.

The topics covered by the course included the following major areas: obtaining sperm from mouse cauda epididymis, obtaining unfertilized mouse oocytes, three different types of in vitro fertilization techniques (using fresh, frozen or refrigerated sperm), vitrification of unfertilized oocytes and 2-cell embryos (freezing and thawing), slow-method for freezing and thawing 2-cell embryos, refrigerated sperm and 2-cell embryos, abdominal and scrotal vasectomies, three types of embryo transfer (oviduct, uterus and non-surgical, with NSET tools), freezing and thawing mouse sperm and ICSI, among many additional common methodologies used to handle mouse embryos and gametes adequately.

The course was very intensive, but the kind atmosphere created by participants and instructors was excellent and, hence, all the tight and carefully devised demonstrations and practices, packed within a very busy schedule, could be run smoothly and successfully without problems. The vast experience in running this type of cryopreservation courses and the remarkable professionality of our colleagues from CARD-University of Kumamoto were key for the accomplished success. All methods followed their three-step learning process. At first, the theory and fundaments were briefly provided and summarized. Then, the method was demonstrated by instructors and, finally, the participants executed the procedures by themselves, with the help of instructors.

The participants left this cryopreservation course to return to their countries and institutions with the most satisfactory results obtained and with plenty of new information to digest, process and reproduce. All participants were given the task to spread the word and disseminate the use of these highly efficient and robust cryopreservation techniques that have boosted the field.

This CARDCNB cryopreservation course was sponsored by the International Society for Transgenic Technologies (ISTT) and received the co-sponsorship and support from a number of companies whose contributions need to be greatly acknowledged as well: Leica, Charles River, Sigma, Labotect, Cosmo-Bio, Kyudo, Harlan and Paratechs.

The new BIOTERIOS.COM web page

Tuesday, July 16th, 2013
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The new BIOTERIOS.COM web page

The new BIOTERIOS.COM web page

BIOTERIOS.COM, the reference web portal on animal experimentation and animal welfare in Spanish in Latin America, created and maintained by Juan Manuel Baamonde (Manager of the animal facility at CECs, Valdivia, Chile, and ISTT Member) since 2007, has launched a new web page, a new layout, to show its very interesting and useful contents with a renewed and modern format. Among the new features that have been added, Juan Manuel must be praised for having included a web page translator tool (found at the top-right corner of all pages) which makes now possible to automatically translate the contents of any web page within BIOTERIOS.COM into another language of choice, to be selected among English, German, French or Portughese, hence further expanding the benefits of this wonderful site to all non-Spanish-speaking colleagues that could not read nor benefit from BIOTERIOS.COM before.
The site includes articles, interviews, reports on recent meetings and plenty of information on animal experimentation and animal welfare issues. Really worth visiting and exploring! (and now in English too!).

Numerous ISTT events in June 2013

Thursday, May 30th, 2013
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Numerous ISTT events in June 2013

Numerous ISTT events in June 2013

The International Society for Transgenic Technologies (ISTT) will be participating and/or co-sponsoring numerous events during the month of June 2013. At first, on June 7, 2013, our ISTT colleagues from Nantes (France), Ignacio Anegon and Séverine Ménoret, experts in the generation of transgenic rats, will be holding their 2013 Nantes Transgenic meeting on “Technical advances in the generation of transgenic animals and in their applications“. Next, on June 10-13, 2013, in Barcelona (Spain), the 12th FELASA-SECAL congress will take place, where the ISTT will be participating in two ways. First, the ISTT will co-sponsor the satellite workshop on Mouse Sperm Cryopreservation, to be held within the 2013 FELASA meeting, on 10 June 2013, Barcelona, Spain, and organized by ISTT members Jorge Sztein (NIH, USA) and Jesús Martínez-Palacio (CIEMAT, Madrid, Spain). Second, the ISTT will be participating as exhibitor and will attend the 2013 FELASA meeting. The ISTT will have a booth in Barcelona (#230), manned by the ISTT administrative assistant, Alison Cameron, and where all ISTT members (and non-ISTT members) are welcome to visiting us. Finally, immediately next, our ISTT colleagues from The Netherlands, Marian Van Roon (VU, Amsterdam) and Sjef Verbeek (LUMC, Leiden), have organized their 2013 Workshop on Innovative Mouse Models (IMM2013). This 7th Workshop on Innovative Mouse Models will be held on 13-14 June 2013, at the Leiden University Medical Center, LUMC, Leiden, The Netherlands, and the ISTT will be co-sponsoring also this event. ISTT members will be entitled to reduced registrations at all these events, proudly co-sponsored by the ISTT.

TT2013 meeting report published in Transgenic Research

Tuesday, April 30th, 2013
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TT2013 meeting report published in Transgenic Research

TT2013 meeting report published in Transgenic Research

The TT2013 meeting report, written by Douglas Strathdee (Beatson Institute for Cancer Research, Glasgow, Scotland, UK) and C. Bruce A. Whitelaw (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) has just been published, online, at the Transgenic Research journal web site. This review, entitled ‘TT2013 meeting report: the Transgenic Technology meeting visits Asia for the first time‘ nicely summarizes the talks and activities held during the recent 11th Transgenic Technology meeting, held in Guangzhou (China), on February 25-27, 2013, along with the subsequent hands-on workshop that was organized, on February 28-March 2, 2013. Douglas and Bruce, together with Peter Hohenstein (Division of Developmental Biology, The Roslin Institute and Royal (Dick) School of Veterinary Studies, University of Edinburgh, Roslin, Midlothian, Scotland, UK) are the Organizers of the next 12th Transgenic Technology meeting, TT2014, which will be held in Edinburgh (Scotland, UK) on October 6-8, 2014.


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