Archive for the ‘coat color’ Category

ISTT co-sponsors Course on Managing Mouse Colonies: Genetics, Breeding & Welfare (6-8 June 2012, WTGC, Hinxton, UK)

Thursday, January 12th, 2012
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ISTT co-sponsors Course on Managing Mouse Colonies: Genetics, Breeding & Welfare (6-8 June 2012, WTGC, Hinxton, UK)

ISTT co-sponsors Course on Managing Mouse Colonies: Genetics, Breeding & Welfare (6-8 June 2012, WTGC, Hinxton, UK)

The International Society for Transgenic Technology (ISTT) is most pleased to announce the co-sponsorship of the 2012 Edition of the popular training Course on Managing Mouse Colonies: Genetics, Breeding & Welfare, organized as a collaborative effort by four institutions: MRC Harwell, the Leeds Institute of Molecular Medicine, the RSPCA Transgenic Training Working Group (TTWG) and the Wellcome Trust Sanger Institute. The course will be held at the Wellcome Trust Genome Campus, Hinxton, Cambridge, UK, on June 6-8 June 2012. Registration deadline: 29 February 2012. ISTT members are entitled to a reduced registration fee.

This training Course aims to introduce experienced technicians and scientific staff involved with the management of  genetically-modified mouse colonies to best practice with respect to the 3Rs and animal welfare. The programme covers historical and current best practice in the maintenance of genetically-modified mouse colonies for scientific research and the differing disciplines involved in production, phenotyping and archiving. Topics covered will include: Topics covered will include: nomenclature, basic colony management, maintaining transgenic and gene-targeted lines, breeding for experimental purposes and maintenance of high health status colonies.

Scientific organisers
James Bussell Wellcome Trust Sanger Institute, UK, ISTT member
Neil Dear Leeds Institute of Molecular Medicine, UK
Nikki Osborne RSPCA, UK
Sara Wells Medical Research Council, Harwell, UK

Keynote speakers
Karen Steel Wellcome Trust Sanger Institute, UK
Ian Jackson Medical Research Council Human Genetics Unit, UK

Confirmed tutors
James Bussell Wellcome Trust Sanger Institute, UK, ISTT member
Neil Dear Leeds Institute of Molecular Medicine, UK
Adrian Deeny University College London, UK
Martin Fray Medical Research Council, Harwell, UK, ISTT member
Richard Houghton Wellcome Trust Sanger Institute, UK, ISTT Member
Natasha Karp Wellcome Trust Sanger Institute, UK
Nikki Osborne RSPCA, UK
Sara Wells Medical Research Council, Harwell, UK
Jacqui White Wellcome Trust Sanger Institute, UK
Ben Woodman Leeds Institute of Molecular Medicine, UK


Advanced Protocols for Animal Transgenesis. An ISTT Manual

Friday, September 9th, 2011
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Advanced Protocols for Animal Transgenesis. An ISTT Manual

Advanced Protocols for Animal Transgenesis. An ISTT Manual

The International Society for Transgenic Technologies (ISTT) is most pleased to announce the publication of its first book, entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thom Saunders, and published by Springer (2011), in collaboration with the ISTT. This laboratory manual provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists, most of them members of the ISTT, who are actively using the technology in their laboratories. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement. This book comprises 26 chapters,  109 illlustrations and 669 pages.

Table of contents, chapters of this ISTT Manual:

  • 1 Patent and Licensing Issues in Transgenic Technology. Karen S. Canady
  • 2 Global Resources: Including Gene Trapped ES Cell. Clones – Is Your Gene Already Knocked Out?. Lluis Montoliu
  • 3 Designing Transgenes for Optimal Expression. Eduardo Moltó, Cristina Vicente-García, and Lluis Montoliu
  • 4 Gene Targeting Vector Design for Embryonic Stem Cell Modifications. Thomas L. Saunders
  • 5 Transgenic Production Benchmarks. Thomas J. Fielder and Lluis Montoliu
  • 6 Generation of Transgenic Mice by Pronuclear Microinjection. Katja Becker and Boris Jerchow
  • 7 Generation of Transgenic Rats Using Microinjection of Plasmid DNA or Lentiviral Vectors. Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal, Anne-Laure Iscache, and Ignacio Anegon
  • 8 Generation of Transgenic Animals by Use of YACs. Almudena Fernández, Diego Muñoz, and Lluis Montoliu
  • 9 BAC Transgenes, DNA Purification, and Transgenic Mouse Production. Michael G. Zeidler, Margaret L. Van Keuren, and Thomas L. Saunders
  • 10 Generation of Transgenic Animals with Lentiviral Vectors. Carlos Lois
  • 11 Vertebrate Transgenesis by Transposition. Aron Geurts, Darius Balciunas, and Lajos Mates
  • 12 Rat Spermatogonial Stem Cell-Mediated Gene Transfer. Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson, and F. Kent Hamra
  • 13 Mouse Cloning by Nuclear Transfer. Sayaka Wakayama, Nguyen Van Thuan, and Teruhiko Wakayama
  • 14 Gene Targeting in Embryonic Stem Cells. Elizabeth D. Hughes and Thomas L. Saunders
  • 15 The Importance of Mouse ES Cell Line Selection. Wojtek Auerbach and Anna B. Auerbach
  • 16 Tetraploid Complementation Assay. Marina Gertsenstein
  • 17 Combining ES Cells with Embryos. Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara, and Marina Gertsenstein
  • 18 Derivation of Murine ES Cell Lines. Kristina Nagy and Jennifer Nichols
  • 19 Rat Embryonic Stem Cell Derivation and Propagation. Ping Li, Eric N. Schulze, Chang Tong, and Qi-Long Ying
  • 20 Induced Pluripotency: Generation of iPS Cells from Mouse Embryonic Fibroblasts. Han Li, Katerina Strati, Verónica Dom?ínguez, Javier Martín, María Blasco, Manuel Serrano, and Sagrario Ortega
  • 21 The Preparation and Analysis of DNA for Use in Transgenic Technology. Anna B. Auerbach, Peter J. Romanienko, and Willie H. Mark
  • 22 Colony Management. Karen Brennan
  • 23 Cryopreservation. Belén Pintado and Juan de Dios Hourcade
  • 24 Shipment of Mice and Embryos. Shirley Pease
  • 25 Pathogen-Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy. Jorge M. Sztein, R.J. Kastenmayer, and K.A. Perdue
  • 26 Refinement, Reduction, and Replacement. Jan Parker-Thornburg

ISTT Members are entitled to purchase this ISTT Manual with a 33% discount, through the members-only area of the web site.

Course on “Genetics of Laboratory Rodents”, Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Saturday, June 4th, 2011
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Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

Course on "Genetics of Laboratory Rodents", Institut Pasteur de Montevideo, Uruguay, December 5-14, 2011

The International Society for Transgenic Technologies (ISTT) is pleased to announce the co-sponsorship of the second edition of the International Course on “Genetics of Laboratory Rodents“, to be held at the Institut Pasteur de Montevideo (IPMONT), Uruguay, on 5-14 December 2011. This course is organized by Martina Crispo (IPMONT, ISTT member) and co-organized by Jean Jacques Panthier (Inst. Pasteur Paris). Following a most successful first edition in 2008, the aim of this course is to offer a training opportunity to South American research scientists and veterinarians in charge of laboratory animal facilities, in the most prominent areas of mammalian genetics (mostly mouse). This course offers an opportunity to receive an intensive training and get in touch with scientist of the region working in the same fields of interest.

The invited speakers at this international 2011 course on “Genetics of Laboratory Rodents” include the following experts in the field:

  • Jean Jacques Panthier (Institut Pasteur de Paris, France)
  • Xavier Montagutelli (Institut Pasteur de Paris, France)
  • Jean Louis Guénet (Institut Pasteur de Paris, France)
  • Jean Jaubert (Institut Pasteur de Paris, France) – ISTT  member
  • Michel Cohen Tannoudji (Institut Pasteur de Paris, France)
  • Ignacio Anegón (Institut de Transplantation-Urologie-Néphrologie,France) – ISTT member
  • Marcelo Rubinstein (Facultad de Ciencias Exactas y Naturales, UBA, Argentina)
  • Andreia Salgado (CEMIB, UNICAMP, Brazil)
  • Fernando Benavides (MD Anderson Cancer Center, Texas, USA) – ISTT member
  • Mariela Bollati (Institut Pasteur de Montevideo)
  • Martina Crispo (Institut Pasteur de Montevideo) – ISTT member

Interested participants should sent their applications by email to: curso-genetica-raton@pasteur.edu.uy by September 25, 2011, and include their CV, letter of motivation and letter of support of their advisors, all in PDF format. Two seats are reserved for qualifying ISTT members. There is no registration fee for this course. The 2011 International Course on “Genetics of Laboratory Rodents” is generously sponsored by CABBIO, AMSUD PASTEUR, Institut Pasteur de Montevideo, ISTT and  Ambassade de France en Uruguay.

Classical videos on transgenic and KO mice from CSHL Press available now to ISTT members

Tuesday, April 26th, 2011
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Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989

Transgenic Techniques in Mice: A Video Guide by Roger A. Pedersen, Janet Rossant, © CSHL Press, 1989

The International Society for Transgenic Technologies is most proud to announce the release of the digital versions of the two classical video guides on transgenic and knockout mice, produced by Cold Spring Harbor Laboratory Press (CSHL Press) in 1989 and 1993, that will be now available to ISTT members for learning and/or teaching purposes, from the ISTT members-only restricted area, thanks to a most generous agreement reached with CSHL Press, owner of the copyright for both videos, marketed as analogic PAL/NTSC videos.

These two videos have been, for many years, unique and the most used and helpful video reference for anyone willing to start learning/teaching in the field of genetically modified mice. The first video is “Transgenic Techniques in Mice: A Video Guide“, by Roger A. Pedersen and Janet Rossant, produced by CSHL Press in 1989. The second video is “Targeted Mutagenesis in Mice: A Video Guide“, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, produced by CSHL Press in 1993.

Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993

Targeted Mutagenesis in Mice: A Video Guide, by Roger A. Pedersen, Virginia Papaioannou, Alexandra Joyner and Janet Rossant, © CSHL Press, 1993

The contents of the two videos are:

Transgenic Techniques in Mice: A Video Guide (CSHL Press, 1989)

  • Procedure 1: Dissection of oviducts. Recovery of fertilized eggs. Removal of cumulus cells.
  • Procedure 2: Recovery of 8-cell embryos. Removal of the zona pellucida. Construction of aggregation chimeras.
  • Procedure 3: Dissection of uteri. Recovery of blastocysts.
  • Procedure 4: DNA injection into pronuclei to produce transgenic embryos.
  • Procedure 5: Nuclear transfer.
  • Procedure 6: Blastocyst injection of embryonic stem cells.
  • Procedure 7: Vasectomizing male mice.
  • Procedure 8: Oviduct transfer of manipulated embryos.
  • Procedure 9: Uterine transfer of manipulated embryos.
  • Procedure 10: Recovery of 6-1/2 and 7-1/2 day embryos.
  • Procedure 11: Dissection of midgestation 12-1/2 day embryos and fetal membranes

Targeted Mutagenesis in Mice: A Video Guide (CSHL Press, 1993)

  • Demonstration 1: Starting embryonic stem (ES) cell lines
  • Demonstration 2: Making fibroblast feeder layers
  • Demonstration 3: Growing established ES cell lines
  • Demonstration 4: Targeting ES cell lines
  • Demonstration 5: Making chimeras with ES cells
  • Demonstration 6: Making a mouse

Advanced Protocols for Animal Transgenesis. An ISTT Manual (Due: August 2011)

Saturday, April 9th, 2011
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Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

Advanced Protocols for Animal Transgenesis. An ISTT Manual. Shirley Pease & Thomas L. Saunders (eds.), Springer 2011, 1st edition (Due: August 2011)

The International Society for Transgenic Technologies (ISTT), in collaboration with Springer, is pleased to announce the next publication of the book entitled “Advanced Protocols for Animal Transgenesis. An ISTT Manual“, edited by Shirley Pease and Thomas L. Saunders, whose 1st edition is expected to be published by August 2011.

This laboratory manual, published by Springer in cooperation with the International Society for Transgenic Technology (ISTT), provides almost all current methods that can be applied to the creation and analysis of genetically modified animals. The chapters have been contributed by leading scientists who are actively using the technology in their laboratories, most of them members of the ISTT. Based on their first-hand experience the authors also provide helpful notes and troubleshooting sections.

Topics range from standard techniques, such as pronuclear microinjection of DNA, to more sophisticated and modern methods, such as the derivation and establishment of embryonic stem (ES) cell lines, with defined inhibitors in cell culture medium. In addition, related topics with relevance to the field are addressed, including global web-based resources, legal issues, colony management, shipment of mice and embryos, and the three R’s: refinement, reduction and replacement.

Table of contents:

  • Karen S. Canady: Patent and licensing issues in transgenic technology.
  • Lluís Montoliu: Global Resources: Including Gene Trapped ES Cell Clones: Is Your Gene Already Knocked Out?.
  • Eduardo Moltó, Cristina Vicente-García and Lluís Montoliu: Designing Transgenes for Optimal Expression.
  • Thomas L. Saunders: Gene Targeting Vector Design for Embryonic Stem Cell Modifications.
  • Thomas J. Fielder and Lluis Montoliu: Transgenic Production Benchmarks.
  • Katja Becker and Boris Jerchow: Generation of Transgenic Mice by Pronuclear Microinjection.
  • Séverine Ménoret, Séverine Remy, Laurent Tesson, Claire Usal , Anne-Laure Iscache  and Ignacio Anegon: Generation of Transgenic Rats using Microinjection of Plasmid DNA or Lentiviral vectors.
  • Almudena Fernández, Diego Muñoz and Lluís Montoliu: Generation of Transgenic Animals by Use of YACs.
  • Michael G. Zeidler, Margaret L. Van Keuren and Thomas L. Saunders: BAC Transgenes, DNA Purification, and Transgenic Mouse Production.
  • Carlos Lois: Generation of Transgenic Animals with Lentiviral Vectors.
  • Aron Geurts, Lajos Mates and Darius Balciunas: Vertebrate Transgenesis by Transposition.
  • Karen M. Chapman, Dalia Saidley-Alsaadi, Andrew E. Syvyk, James R. Shirley, Lindsay M. Thompson and F. Kent Hamra: Rat Spermatogonial Stem Cell Mediated Gene Transfer.
  • Sayaka Wakayama, Nguyen Van Thuan and Teruhiko Wakayama: Mouse Cloning by Nuclear Transfer.
  • Elizabeth D. Hughes and Thomas L. Saunders: Gene Targeting in Embryonic Stem Cells.
  • Wojtek Auerbach and Anna B. Auerbach: The Importance of Mouse ES Cell Line Selection.
  • Marina Gertsenstein: Tetraploid Complementation Assay.
  • Elizabeth Williams, Wojtek Auerbach, Thomas M. DeChiara and Marina Gertsenstein: Combining ES cells with Embryos.
  • Kristina Nagy and Jennifer Nichols: Derivation of Murine ES Cell Lines.
  • Ping Li, Eric N Schulze, Chang Tong and Qi-Long Ying: Rat Embryonic Stem Cell Derivation and Propagation.
  • Han Li, Katerina Strati, Verónica Domínguez, Javier Martín, María Blasco, Manuel Serrano and Sagrario Ortega: Induced pluripotency: generation of iPS cells from mouse embryonic fibroblasts.
  • Anna B. Auerbach, Peter J. Romanienko and Willie H. Mark: The Preparation and Analysis of DNA for Use in Transgenic Technology.
  • Karen Brennan: Colony Management.
  • Belen Pintado and Juan Hourcade: Cryopreservation.
  • Shirley Pease: Shipment of Mice and Embryos.
  • Jorge M. Sztein, R.J. Kastenmayer and K.A. Perdue: Pathogen Free Mouse Rederivation by IVF, Natural Mating and Hysterectomy.
  • Jan Parker-Thornburg: Refinement, Reduction and Replacement

ISTT Members are entitled to a 33% discount on the book price.

ISTT and Transgenic Research jointly promote the use of standard nomenclature

Tuesday, December 7th, 2010
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Examples of use of standard nomenclature [see Montoliu & Whitelaw (2010) for a full descrption]

Examples of use of standard nomenclature: see Montoliu & Whitelaw (2010) for a full descrption

The International Society for Transgenic Technologies (ISTT) and the scientific journal, Transgenic Research, to which the ISTT is associated, have decided to promote the use of standard nomenclature for naming mouse strains, genes, mutations and alleles. In this regard, and according to ISTT aims and activities, ISTT will disseminate in its web site, blog, newsletter and Transgenic Technology (TT) meetings, information and recommendations regarding how to use standard nomenclature. Likewise, the correct use of rules and guidelines for standard nomenclature for naming mouse strains, genes, mutations and alleles will be enforced in subsequent articles submitted to Transgenic Research for publication, as it is already done in several other journals.

Joint Position Article (ISTT and Transgenic Research):

Montoliu Lluis & Whitelaw C. Bruce A. (2010) Using standard nomenclature to adequately name transgenes, knockout gene alleles and any mutation associated to a genetically modified mouse strain. Transgenic Research (ePub, 16 July 2010) [this article has been made freely available by Springer]

B6N ES cells can be aggregated with albino outbred ICR morulas for the efficient production of chimeras

Friday, July 16th, 2010
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ES cells set for aggregation with a mouse morula

ES cells set for aggregation with a mouse morula

Marina Gertsenstein (member of ISTT) and her colleagues, from the Samuel Lunenfeld Research Institute, Mount Sinai Hospital and the Toronto Centre for Phenogenomics, in Toronto (ON, Canada), have recently published a scientific article in PLoS ONE describing the efficient generation of mouse chimeras with C57BL/6N ES cells by aggregation with standard albino outbred ICR morulas. As detailed in this work, the use of chemically-defined ES cell culture medium (2i) appears to be crucial for the success of the experiment. This is an interesting development that can help the biomedical research community for the easy and rapid generation of C57BL/6N -derived chimeras at a reduced cost. It also complements the toolbox by which chimeras can be obtained with the popular C57BL/6N ES cells, used in the large-scale international consortiums (i.e. IKMC) aiming to generate systematic knock-outs of all genes in the mouse genome.

White creamy chimeras at the ISTT collection of mouse pictures

Saturday, June 26th, 2010
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Mouse chimera generated by injecting E14 ES cells into albino blastocysts (picture by Sergey Kupriyanov)

Mouse chimera generated by injecting E14 ES cells into albino blastocysts (picture by Sergey Kupriyanov)

New images have been added to the ISTT collection of mouse pictures. Our colleague, Sergey Kupriyanov (The Scripps Research Institute, Mouse Genetics Core, La Jolla, CA, USA), has contributed with new pictures of beautiful “white creamy” mouse chimeras, obtained by injecting E14 ES cells into an albino blastocyst. Up to 264 pictures and 60 videos are now available from the ISTT web site, members-only section, illustrating different aspects of the generation and analysis of transgenic animals. This great multimedia resource is most appreciated for teaching and learning purposes.

The colors of mice: new book published on genetics of pigmentation in mice

Thursday, June 17th, 2010
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The colors of mice

The colors of mice

A new book on genetics of mouse pigmentation has been just published, entitled  ”The colors of mice: a model genetic network” by M. Lynn Lamoreux, Véronique Delmas, Lionel Larue and Dorothy C. Bennett (Wiley, 2010). According to publisher’s page, this new book “… showcases a blend of new technologies and new insights in the field of pigmentary genetics of mice, with comparative information on other animals…”

Genetic polymorphisms among C57BL/6 mouse inbred strains

Monday, May 31st, 2010
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Genetic polymorphisms among C57BL/6 mouse inbred strains

Genetic polymorphisms among C57BL/6 mouse inbred strains

A manuscript entitled “Genetic polymorphisms among C57BL/6 mouse inbred strains” has been just published by Esther Zurita, Mónica Chagoyen, Marta Cantero, Rosario Alonso, Anna González-Neira, Alejandro López-Jiménez, José Antonio López-Moreno, Carlisle P. Landel, Javier Benítez, Florencio Pazos and Lluís Montoliu in Transgenic Research. The Illumina® Mouse Medium Density Linkage Mapping panel, with 1,449 single nucleotide polymorphisms (SNPs), was used to genotype individuals from ten C57BL/6-related strains: C57BL/6JArc, C57BL/6J from The Jackson Lab, C57BL/6J from Crl, C57BL6/JRccHsd, C57BL/6JOlaHsd, C57BL/6JBomTac, B6(Cg)-Tyr (<c-2j>)/J, C57BL/6NCrl, C57BL/6NHsd and C57BL/6NTac. Twelve SNPs were found informative to discriminate between the “/N” and “/J”  C57BL/6 mouse substrains considered. These results will be instrumental for the correct genetic monitoring and appropriate mouse colony handling of different transgenic and knockout mice produced in distinct C57BL/6 inbred substrains. The SNP raw data are also available from the Mouse Phenome Database at Jackson Laboratory.


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