Newly elected 2016 ISTT Board of Directors members

September 1st, 2015
LinkedIn

ISTT BOD electees

   Branko Zevnik      Lynn Doglio      Peter Hohenstein

The official results of the recent election for the ISTT Board of Directors are in, and we congratulate Branko Zevnik, Lynn Doglio and Peter Hohenstein on their election to the ISTT Board. Branko Zevnik (Cologne, Germany) is the head of the in vivo Research Facility at the University of CologneLynn Doglio (Chicago, USA) is the Director of the Transgenic and Targeted Mutagenesis Laboratory at Northwestern University.  Peter Hohenstein (Edinburgh, United Kingdom) is a Group Leader and Chair of the Small Animal Facility Management Committee at The Roslin Institute.  All three candidates received the support of the majority of voting ISTT members.  While they will start their three-year terms at TT2016 to be held in Prague, Czech Republic, they will be immediately appointed as Board members-elect.  This will allow them to interact with the rest of the ISTT Board members and become familiar with the administration and management of the ISTT. Congratulations to all of them!

In addition, the ISTT would like to express its respect and sincere appreciation for the commitment and participation of all the candidates who were involved in the ISTT election process.  As well, we would like to thank Tom Fielder, Wojtek Auerbach, and Boris Jerchow for their service on the  ISTT Council and Board of Directors.  These three Board members have contributed greatly to the management of the ISTT (and will continue to do so until TT2016).  It is due in large part to the contributions of our members that we have the vibrant Society that we do.

TARC X Meeting Report

August 26th, 2015
LinkedIn

20150809_084915 20150817_103359 20130810_163007Tahoe City, California, USA

August 9 – 13, 2015

“What if . . . we had cows that did not have horns?  We do!  This is a naturally-occurring mutation, and these are called “polled” (or, hornless) cows.  This is a great benefit to the cattle industry, as this reduces the amount of trauma that cows can cause each other.  Unfortunately, there are only a few types of cows that contain the mutation causing the polled phenotype.  Other cows must have their horns removed to safely interact with each other in groups and their handlers.  You can see that this type of “surgery” could also cause animal welfare issues.

But, what if we could transfer the naturally-occurring mutation from one type of cow to another?  This can be accomplished by breeding the mutation into non-polled cattle.  Keeping in mind that the time for gestation in cattle is 9 months, and then the time to sexual maturity could be another one to one and a half years, the time needed to do the number of crosses to generate this mutation in a new strain of cattle could be significant—one breeder’s lifetime.  But (again, another “but”), what if we could introduce this mutation in a single generation by genetic engineering and leave no footprint behind—just this ONE MUTATION.  It is now possible to do this using the CRISPR/Cas9 system; one could introduce the mutation and carefully characterize the animals that result to insure that there are no additional changes in the genome—no footprints.  You could argue that this would be incredibly beneficial for animal welfare issues and for the benefit of those who care for these animals.”

This is the type of discussion that can result, based on the research presented at the  Tenth Transgenic Animal Research Conference (TARC X) [http://www.cevs.ucdavis.edu/confreg/?confid=732] just completed in Lake Tahoe, California, USA.  The discussions and talks centered around transgenic animals other than mice, including cows, sheep, goats and pigs, as well as avians (chickens), rabbits, and even mosquitoes!  An especially valuable addition to the signature 10th Conference was the inclusion of reviews of different aspects of the technology given at the start of each session.

In the first session, Dr. Jim Murray (UC Davis, USA) reviewed how genetically engineered livestock have been developed for agriculture since the first TARC meeting in 1997.  This was closely followed by a talk from Maeve Ballantyne (Roslin Institute, Scotland) about their efforts to engineer resilience to African swine fever into pigs.  This disease is rapidly spreading from Africa throughout Eastern Europe.  Thus, this type of genetic engineering could be critical for maintaining the health of swine herds.  The following talk by Jayne Raper (CUNY, USA), was a natural extension in this session, discussing how genes encoding resistance to trypanosomiasis in non-human primates could be moved into sheep and cattle.  The expectation is that such genes are critical for maintaining the health of these herds throughout Africa.

The second session was devoted to new technologies for genome engineering.  It started with an excellent review from Bruce Whitelaw (Roslin Institute, Scotland).  His review showed how the initial slow progress in generating precisely mutated animals has become much more rapid with the introduction of genome editing.  The promise of this technology was soon demonstrated by Mark Tizard (CSIRO, Australia), who described efforts to edit the genome of poultry, and by Bhanu Teluga (University of Maryland, USA), who described his highly efficient CRISPR/Cas targeted genome editing in pigs.

After an afternoon break for hiking, shopping, boating and general fun in Lake Tahoe, there was a late afternoon poster session with submissions from throughout the world.  After dinner, the evening session began with a talk from Pablo Ross (UC Davis).  Pablo reviewed how pluripotent stem cells have been used to generate targeted livestock, and tantalized the audience with a promise of an upcoming publication describing a new media for growth of pluripotent stem cells from large animals, hopefully capable of generating chimeras and germline transmission.  This was closely followed by talks from Franklin West (Univ. of Georgia, USA) and Jorge Piedrahita (NCSU, USA) about the use of stem cells in both pigs and chickens.

The second full day of the meeting was begun with a review by Chris Rogers (Exemplar Genetics, USA) on how genetically engineered livestock have been developed for biomedical models.  Simon Bawden (SARI, AU) reported how Huntington’s disease has been recreated in sheep.  This was followed by a talk from Lydia Garas (UC Davis, USA) about lysozyme transgenic goats whose milk can be used to prevent and treat intestinal diseases.  After a short break, Mingjun Liu (China) described how the sheep FGF5 and MSTN genes have been altered using CRISPR/Cas9 gene editing.  The final talk of the morning was from Margareth Capurro (Univ. of Sao Paulo, Brazil), where she captivated the audience with her description of the methods used to gain acceptance for release of GE mosquitoes to reduce the incidence of dengue fever in one Brazilian village.  Margareth finished her talk with a most memorable jingle used as a public service announcement!

The Tuesday afternoon session was composed of talks from Eddie Sullivan (SABBiotherapeutics, USA) about the generation of humanized antibodies produced in cows, and from Lissa Herron (Roslin Institute, Scotland) about the isolation of pharmaceutical proteins from avian egg whites.  These talks were then followed by an enthusiastic review from Tim Doran (CSIRO, AU) where he surveyed the advances made in engineering of the avian genome.  A number of conference attendees added to their notoriety by being listed in his “Hall of Fame”!  The final talk on Tuesday, given by Marie-Cecile van de Lavoir (Crystal Biosciences, USA), described the generation of transgenic chickens carrying Cre-recombinase, which can be used to delete selectable markers in vivo.

The final day of the regular conference began with a review by Kevin Wells (Univ. of Missouri, USA) of the regulations governing genetic engineered animals and the food supply.  He emphasized that, in the US, while there are regulations that apply, there have not been laws passed that oversee this area, and he called for the preparation of a “white paper” by the experts in the field to advise the US government.  His talk was followed by a presentation of the “Glo-fish”@ experience with obtaining US approval given by Alan Blake (Yorktown Technologies, USA).  William Muir (Purdue Univ., USA) then presented his statistical model (Hazard Assessment at Critical Control Points, or HAACP) that can assess environmental risk of GE animals based on net fitness of the organism, demonstrating its effectiveness in an experiment on a model organism.  He then showed its application to the Aquabounty@ salmon currently awaiting approval, showing that the fear of an accidental release is irrelevant, as the GE salmon would quickly be eliminated from the population.

The next session had talks from Jun Wu (Salk Institute, USA) on the development of pluripotent stem cells, and their use in the pig to generate humanized organs for transplant; and from Hiro Nakauchi (Stanford Univ., USA) on exploiting an “organ niche” by injecting pluripotent stem cells from one organism (rat) into another, deficient organism (Pdx1-/- mouse) to generate a xenogenic pancreas.  He is now testing this process in pigs as well.

Attendees were then given another welcome afternoon off to play in the surrounding area, where there is ample opportunity for boating, biking and hiking.  This being the final day of the regular conference, everyone truly welcomed this last chance to enjoy the lake and surrounding mountains.

The final session of the meeting began (after another poster session and dinner) with a review given by Heiner Niemann (Hannover, Germany), where he spoke about the use of pigs as xeno-donors for human organs.  He described three major hurdles to this scenario, including immune responses, physiological incompatibilities, and the risk of transmitting zoonotic organisms.  His own work is an attempt to modify the immune response by humanizing several candidate genes.

The last talk of the meeting was from Alison Van Eenennaam (UC-Davis, USA) about how the technology has progressed but the acceptance of transgenic food animals has not over the past twenty years that TARC meetings have been held.  She made an eloquent request that scientists take the time to explain and assure the public that genetic engineering technology can be safe and assist the world with developing a healthy, sustainable food supply.  The scientific portion of the meeting then ended with the presentation of the poster award (sponsored by the Roslin Institute) to Dorothea Aumann (Munich, Germany) for her poster on “Analyzing gamma/delta T-cell function in chicken by reverse genetics”.  The award presentation was followed by a discussion of how to advance the regulatory environment.

An optional Livestock Industry Day was held the following day, 14 August, 2015, where various company representatives could share their work, interact with attending scientists, and have another enjoyable day in Lake Tahoe.  All in all, it was a very informative, interesting, and pleasurable meeting.   Granlibakken Conference Center [http://www.granlibakken.com], The UC Davis Department of Animal Science [http://animalscience.ucdavis.edu], Drs. Jim Murray, Elizabeth Maga, Alison Van Eenennaam and Pablo Ross should be commended for their hard work in producing such a successful gathering.  The next meeting will be held August 13-17, 2017—please plan on attending!

 

 

Respectfully submitted by:
Jan Parker-Thornburg, with editing from Walter Tsark and Jim Murray

Fourth ISTT Young Investigator Award

August 4th, 2015
LinkedIn

ISTTlogo2ISTT-ingenious

The International Society for Transgenic Technologies (ISTT), in collaboration with ingenious targeting laboratory, has established the ISTT YOUNG INVESTIGATOR AWARD that is presented at each Transgenic Technology meeting. The fourth edition of this ISTT Young Investigator Award will be given at the next 13th Transgenic Technology Meeting (TT2016), that will be held in Prague, on 20-23 March 2016.

The ISTT Young Investigator Award recognizes outstanding achievements by a young scientist who will keep the field of transgenic technologies vibrant with new ideas and who has recently received his or her advanced professional degree. The ISTT Young Investigator Award is generously sponsored by ingenious targeting laboratory.

To date, the ISTT Young Investigator Awards has been granted to Dr. Xiao-Yang Zhao (TT2011), Dr. Toru Takeo (TT2013) and Dr. Feng Zhang (TT2014).

The ISTT Young Investigator Award is associated to funds that will serve to defray the costs related to the participation of the ISTT Young Investigator awardee at the corresponding TT Meeting, including: TT meeting registration fee, travel expenses to the TT meeting venue and accommodation during the meeting dates for the awarded candidate, with a maximum being set to 1500 Euros. All ISTT Young Investigator awardees will be given 1 year Ordinary ISTT Membership and a diploma, that will be provided at the ISTT Awards Ceremony, within the TT Meeting Program.

Instructions for participating and additional information are provided at the ISTT Young Investigator Award web page, within the ISTT web site.

ELEGIBILITY criteria for ISTT Young Investigator Awards

Nominees must be active in research, in the field of transgenic technologies, at the time the award is given. Nominees must have received a PhD or MD (or equivalent) within the past 10 years.

NOMINATION criteria for ISTT Young Investigator Awards

Nominations must be made or endorsed by an ISTT member. Nominee does not have to be an ISTT member. Self nomination is not permitted. No person may nominate more than one candidate.

PROCEDURE for participating in the ISTT Young Investigator Awards selection process

Nominations must be made or endorsed by an ISTT member and should include:

  •                the CV of the nominee
  •                a document highlighting the achievements made by the nominee in the field of transgenic technologies
  •                a message or document confirming the nominee’s acceptance to participate in the ISTT Young Investigator Award selection process

All documents should be sent by the ISTT Member nominating the candidate to the ISTT email address: istt@transtechsociety.org

Currently we are accepting nominations for the 4th ISTT Young Investigator Award for the TT2016 meeting in Prague.

DEADLINE for submitting nominations: September 15, 2015.

Decision for the TT2014 ISTT Young Investigator Award will be communicated by October 15, 2015.

On behalf of the ISTT, I would like to thank ingenious targeting laboratory for their support to the ISTT and, in particular, for sponsoring this 4th  ISTT Young Investigator Award.

With my best regards, Benoît Kanzler, ISTT Vice-President

ISTT Registration Awards for TT2016 meeting in Prague

July 27th, 2015
LinkedIn
ISTT Registration Awards for TT2016 meeting in Prague

ISTT Registration Awards for TT2016 meeting in Prague

A minimum of six Registration Awards will be sponsored by the International Society for Transgenic Technologies for ISTT members wishing to attend the 13th Transgenic Technologies (TT2016) meeting in Prague, The Czech Republic, on 20-23rd March, 2016. Selected applicants will be awarded funding to cover registration fees plus attendance at all social events. However, the award does not cover travel expenses, hotel accommodation or attendance at pre-meeting events.

Applicants who are not yet members of the ISTT may join the ISTT and simultaneously submit their Registration Award application. Only those applications from members who have paid their current annual fees will be considered.

Procedure
Applicants must register first at the TT2016 Meeting website and select, as a payment method, “Application for Registration Awards” as a payment method. The ISTT will pay the Registration Fee of all applicants selected for an award. Applicants not selected will be kindly requested to pay the corresponding registration fee.
Applications and additional required documents (see below) should be sent, along with the meeting registration confirmation, to the official ISTT email address, istt@transtechsociety.org, by 30th November, 2015.

Additional Documentation Required
a) Applicant’s CV
b) For ordinary members, a letter from the applicant describing how he/she will benefit from attending the TT meeting
c) For technician/student members, a letter of support from the applicant’s PI or supervisor stating how attendance will benefit the applicant’s career

Selection Process
ISTT Registration awards will be selected by a subset of ISTT Council members, with preference given to:
1) Student/Technician ISTT Members
2) ISTT Members submitting an abstract for presentation as a poster/short-oral communication at the TT meeting
3) Any other ISTT Member

Decision
Awards to the selected applicants will be announced by December 15th, 2015 and awardees will receive a diploma marking the event at the end of the TT2016 Meeting.

Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

July 4th, 2015
LinkedIn
Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

Registration OPEN for the next (13th) Transgenic Technology (TT2016) meeting in Prague, Czech Republic, 20-23 March 2016

Dear colleagues,

we are pleased to announce that registration and abstract submission for the 13th Transgenic Technology Meeting (TT2016) in Prague, Czech Republic from 20th – 23rd March 2016 are now OPEN.

At TT2016, Jennifer Doudna and Emmanuelle Charpentier will be jointly awarded the 11th ISTT Prize. Andras Nagy will give the opening plenary lecture and Denis Duboule the closing one.
Thomas Boehm and Richard Behringer will give our special Orbis pictus lectures.

Further information can be found at the conference website.

We look forward to seeing you here in the beautiful city of Prague.

Radislav Sedlacek
Chair of the TT2016 Organizing Committee

Tenth Transgenic Animal Research Conference, Tahoe City, California (USA), 9 – 13 August, 2015

June 26th, 2015
LinkedIn

TARC_2015blog

Plan to attend the 10th Transgenic Animal Research Conference (TARC X) in August of 2015.  At this international meeting you will learn the latest developments in the field of non-murine transgenic animals. In celebration of the 10th conference in this series the program will contain nine review talks, to be published in a special issue of Transgenic Research. Once again the conference will be held at the beautiful Granlibakken Resort and Conference Center, high in the Sierra Nevada Mountains adjacent to beautiful Lake Tahoe. This meeting is co-sponsored by the ISTT.

Rooms are limited, so plan to register early. The conference web site opened February 1, 2015 for registrations and submission of poster abstracts. The following list of speakers confirms again that this is conference not to be missed. Additionally, in conjunction with Recombinetics, Inc there will be a special one day program on August 13th for the livestock, poultry and aquaculture industries on the application of GE animals. A list of confirmed speakers and topics, as well as additional information, registration and poster submission forms may be found on the conference web site (http://conferences.ucdavis.edu/transgenic).  We invite you to join us for this interesting and important conference and learn more about the genetic future of the livestock industry.

Confirmed Speakers:

Reviews

  • Elizabeth Maga/Jim Murray (UC Davis)  GE livestock for agriculture
  • Chris Rogers (Exemplar Genetics)  GE livestock for biomedical models
  • Heiner Niemann (Hannover) Xenotransplantation
  • Tim Doran (CSIRO, Australia) GE Poultry
  • Pablo Ross (UC Davis)  iPS/Stem cells
  • Jun Wu (Salk Institute) Organ complementation
  • Bruce Whitelaw (Edinburgh) Gene editing/gene targeting
  • Luciana Bertolini (Brazil) Production of pharmaceuticals
  • Kevin Wells  (Missouri) Regulation of transgenic animals

Additional speakers and topics

  • Maeve Ballantyne  (Roslin) African swine fever resistant pigs
  • Simon Bawden  (Australia) Huntington’s disease sheep model
  • Jayne Raper  (New York)  Cattle resistant to trypanosomiasis
  • Lydia Garas (UC Davis)  Effects of lysozyme milk on intestinal health
  • Jorge Piedrahita (NC State)  SCID pigs
  • Margarthe Cupurra (Sao Paulo)  GE mosquitos to control dengue fever
  • Lissa Herron (Roslin)  Pharmaceuticals from eggs
  • Eddie Sullivan (SABBiotherapeutics)  Targeting emerging infectious diseases through animal biotechnology
  • Hiro Nakauchi (Stanford) Interspecies blastocyst complementation

IMM2015-Leiden, June 11-12, 2015-A Short Meeting Report

June 15th, 2015
LinkedIn
IMM2015-Leiden, June 11-12, 2015-A Short Meeting Report

IMM2015-Leiden, June 11-12, 2015-A Short Meeting Report

On June 11-12, the 8th Workshop on Innovative Mouse Models (IMM2015) was held in the Leiden University Medical Center, Leiden, Netherlands. This biannual meeting brings together a diverse group of researchers interested in developing and exploiting mouse models to study fundamental developmental processes and to mimic human disease. Featuring the most recent advances on transgenic animal technology, this meeting encourages in-depth discussions in a very open way, accessible for young and senior scientists. The local organizers proposed a very attractive program composed of 11 keynote lectures, 10 oral selected presentations and a forum discussion on the impact of new transgenic technology advances. About 150 scientists shared data, frustrations and promising future designs of recent transgenic approaches, particularly exploring the future and limits of the extremely powerful CRISPR/Cas9 system for genome editing. Also discussed were improved mouse reproductive technologies (sperm cryopreservation, embryo production…), novel imaging-technologies and a new and very efficient way of delivering native proteins.
Sjef Verbeek, initiator of the IMM worshops, opened the workshop with a warm welcome for all participants and expressed his gratitude to all the sponsors: Innoser, the International Society for Transgenic Technologies (ISTT), Leiden University Medical Center (LUMC) and The Netherlands Cancer Institute (NKI).
While we were proud to notice that many participants were already ISTT members, we believe that our presence there as a sponsor with a booth could convince many more to take THE step forward and join our society, as well as join us at our next TTMeeting in Prague (TT2016, 20-23 March 2016)! Yes, the ISTT booth attracted many scientists interested in our role and activities and has definitely been a central meeting point of IMM2015!
Benoît Kanzler

ISTT Board of Directors: STATEMENT ON GENETIC ENGINEERING OF HUMAN EMBRYOS

June 10th, 2015
LinkedIn

ISTT Board of Directors

STATEMENT ON GENETIC ENGINEERING OF HUMAN EMBRYOS

June 10, 2015

Genetic engineering in animals is a process that has engendered great excitement as well as great anxiety.  The technology is used to study developmental processes (using small animals such as the mouse, zebra fish, fruit fly, worm, etc.), determine gene function, and mimic human and animal disease processes.  Perhaps the greatest promises of this technology are to develop and test drugs and to perform gene therapy, both of which are intended to prevent or cure disease.  

Until recently, a variety of limitations made the technology impractical for all but a few species of animals (primarily mice).  However, with the advent of new gene-editing systems, where components are inexpensive, readily generated in the laboratory, and applicable to virtually any species, it is now feasible to perform genetic engineering in the human embryo.  Changes made in an embryo brought to term would no longer be confined to that individual, but could be passed through the germline to affect future generations.

A recent publication [Liang, P. et al. Protein Cellhttp://dx.doi.org/10.1007/s13238-015-0153-5 (2015)] brought this reality squarely into the public consciousness.  In this study, the CRISPR/Cas9 system was used to edit the genome of human embryos.  To their credit, the authors were careful to use only non-viable embryos.  Furthermore, their detailed examination of the engineered embryos revealed both the intended and unintended modifications that resulted.  This study clearly demonstrates that the CRISPR/Cas9 system is currently too imprecise and inefficient for genetic engineering of human embryos for implantation, gestation and birth.  

Members of the ISTT use CRISPR/Cas9 technology, as well as other gene-editing technologies, routinely.  Many of our members have had integral roles in the development of these technologies and therefore recognize the power of these systems.  It is with that knowledge and foresight that the ISTT Board of Directors issues this statement (while understanding that more nuanced discussions and decisions will be needed as the technology improves):

  • Genetic engineering technology, in its current state, is error-prone and must not be used in human embryos intended for implantation.
  • Studies to test new genetic engineering technology in human embryos should be postponed until proven completely safe and effective in other species.
  • New methods of genetic engineering must be carefully assessed to ensure that risk to the human population is negligible.
  • Uses of genetic engineering in human embryos should be limited to disease mitigation for those diseases where no other option is available; we reject the idea of “designer babies.”
  • We strongly urge worldwide agreement on minimum standards for gene editing experiments in human embryos, and will promote such measures with our members.  Until such standards have been established, we remain opposed to making any genetic alterations in human embryos that could be inherited by future generations.

 

Ultra-superovulation in C57BL/6 mice: 100 oocytes/female

May 30th, 2015
LinkedIn
New achievement in Reproductive Biology by Toru Takeo & Naomi Nakagata (CARD, University of Kumamoto, Japan). Ultra-superovulation of C57BL/6 mice: 100 oocytes/female obtained priming the animals with equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS)

New achievement in Reproductive Biology by Toru Takeo & Naomi Nakagata (CARD, University of Kumamoto, Japan). Ultra-superovulation of C57BL/6 mice: 100 oocytes/female obtained priming the animals with equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS)

Last September, in Buffalo (USA), at the CARD-RPCI Mouse Sperm and Embryo Cryopreservation course organized by Naomi Nakagata, Aimee Stablewski and Jan Parker-Thornburg, Naomi Nakagata himself presented the preliminary results of an amazing achievement in Reproductive Biology they had accomplished at the University of Kumamoto (Japan), namely: the obtention of more than 100 oocytes per C57BL/6 female after devising a new protocol for superovulation, a method they introduced as ULTRA-superovulation. Now, these totally unexpected results see the light in the form of a scientific manuscript, published yesterday in the PLOS ONE journal:

Toru Takeo & Naomi Nakagata (2015) Superovulation Using the Combined Administration of Inhibin Antiserum and Equine Chorionic Gonadotropin Increases the Number of Ovulated Oocytes in C57BL/6 Female Mice. PLOS ONE, Published: May 29, 2015DOI: 10.1371/journal.pone.0128330

In brief, in this publication, Toru Takeo and Naomi Nakagata describe their superovulation results using young (4-weeks old) C57BL/6 female after envisaging a new priming protocol. The combined used of equine chorionic gonadotropin (eCG) and inhibin antiserum (IAS), in a protocol they call IASe treatment,  significantly increased the number of oocytes obtained per C57BL/6 females. On average, more than 100 oocytes/female were obtained, about 3-4 times the number of oocytes regularly obtained by classical superovulation protocols. Thereafter, the authors tested the quality of these oocytes and used them for IVF, obtaining high fertilization rates (~90%), comparable to the high values regularly obtained with the new CARD methods these authors also devised recently, which have boosted the field of cryopreservation of mutant mice. Furthermore, the authors verified that the number of pups obtained after transferring all these embryos, obtained from IASe-derived oocytes and IVF  into recipients, was also 2-3 times higher.

The CARD YouTube channel has also released a movie showing ampullas of oviducts from IASe-treated C57BL/6 females literally full of oocytes.

The IAS reagent used by Takeo & Nakagata is not yet commercially available. In the paper, the authors produced the IAS by themselves and titrated the product until finding the optimal dose required for maximum output. On the contrary, eCG is commercially available and is commonly used in all mouse reproductive biology and transgenic labs to promote follicle growth. Subsequent experiments will be needed to explore the validity of these results in other mouse strains and species. In addition, a commercial reliable and validated source of IAS will greatly facilitate the dissemination of this new ultra-superovulation method among the scientific community. It is also remarkable to note that the application of the IASe treatment will logically reduce the number of superovulated donor females required to obtain oocytes for cryopreservation/IVF purposes, as nicely demonstrated in this first publication, and, likely, for other aims (i.e. microinjection of DNA or RNA/genome editors to produce genetically altered mice).

Congratulations once again to Toru Takeo and Naomi Nakagata for these impressing results and for their new spectacular achievement in mouse reproductive biology!.

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015)

May 22nd, 2015
LinkedIn
Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015), 11-12 June 2015, in Leiden, the Netherlands

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015), 11-12 June 2015, in Leiden, the Netherlands

Meet the ISTT at the 8th Workshop on Innovative Mouse Models (IMM2015) that will be held on 11-12 June 2015, in Leiden, the Netherlands. The popular biannual “Workshop on Innovative Mouse Models” brings together a diverse group of scientists interested in developing and exploiting mouse models to study fundamental developmental processes and to mimic human disease. Keynote speakers from leading laboratories present the latest developments on advanced genome alteration protocols, this year specifically focusing on the use of CRISPR/Cas9-assisted gene modification. Also novel imaging-technologies will be presented. The two-day workshop format combines keynote lectures and presentations of selected abstracts in order to encourage in-depth and unvarnished discussions of novel technologies.
For more information, scientific programme with confirmed speakers and registration, please visit: http://research.nki.nl/immworkshop/
Looking forward to meet many of you at IMM2015 in Leiden!


This blog is protected by Dave\\\'s Spam Karma 2: 11327 Spams eaten and counting...